Bekir Dizman

Synthesis, characterization, and antibacterial activities of novel methacrylate polymers containing norfloxacin.

A novel methacrylate monomer containing a quinolone moiety was synthesized and homopolymerized in N,N-dimethylformamide (DMF) by using azobisisobutyronitrile (AIBN) as an initiator. The new monomer was copolymerized with poly(ethylene glycol) methyl ether methacrylate (MPEGMA) in DMF using the same initiator. The monomer, homopolymer, and copolymer were characterized by elemental analysis, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), size exclusion chromatography (SEC), FTIR, (13)C NMR, and (1)H NMR. The antibacterial activities of the monomer as well as polymers were investigated against Staphylococcus aureus and Escherichia coli, which are representative of Gram-positive and Gram-negative bacteria, respectively. All compounds showed excellent antibacterial activities against these two types of bacteria. The antibacterial activities were determined using the shaking flask method, where 25 mg/mL concentrations of each compound were tested against 10(5) CFU/mL bacteria solutions. The number of viable bacteria was calculated by using the spread plate method, where 100 microL of the incubated antibacterial agent in bacteria solutions were spread on agar plates and the number of viable bacteria was counted after 24 h of incubation period at 37 degrees C.

Synthesis and antibacterial activities of new quaternary ammonium monomers

New monomers and polymers with amine and quaternary ammonium groups on the side chain were synthesized. The first series of monomers were the derivatives of 3-(acryloyloxy)-2-hydroxypropyl methacrylate (AHM). Hydroxylated secondary and tertiary amine derivatives were prepared by reaction of AHM with various amines. One of the amine monomers was quaternized with bromohexane to give a quaternary ammonium monomer. A second series of amine and quaternary ammonium monomers were based on acrylamide and methacrylamide derivatives. New acrylamide/methacrylamide monomers with tertiary amine groups were synthesized and quaternized with various alkyl halides. All monomers were homo-polymerized and co-polymerized with 2-hydroxyethylmethacrylate (HEMA). The synthesized monomers and polymers were tested for antibacterial activities against Staphylococcus aureus and Escherichia coli bacteria. Amine monomers, their homo-polymers and co-polymers did not show any antibacterial activities. It was also found that the antibacterial activity of the quaternized methacrylamide-3-(aminomethyl) pyridine (METH-PYR) monomers increased as the alkyl chain length on nitrogen increased.