Belsy Acosta Herrera

Molecular and phylogenetic analysis of influenza A H1N1 pandemic viruses in Cuba, May 2009 to August 2010

The influenza A(H1N1)pdm09 virus was detected in Cuba in May 2009. The introduction of a new virus with increased transmissibility into a population makes surveillance of the pandemic strain to the molecular level necessary. The aim of the present study was the molecular and phylogenetic analysis of pandemic influenza A(H1N1)pdm09 strains that circulated in Cuba between May 2009 and August 2010. Seventy clinical samples were included in the study. Nucleotide sequences from the hemagglutinin HA1 region segment were obtained directly from clinical samples. Genetic distances were calculated using MEGA v.5.05. A phylogenetic tree was constructed using MrBayes v.3.1.2 software. Potential N-glycosylation sites were predicted using NetNGlyc server 1.0. The 48 Cuban sequences of influenza A(H1N1)pdm09 obtained were similar to the A/California/07/2009 (H1N1) vaccine strain. Most of the Cuban strains belonged to clade 7. Cuban viruses showed amino acid changes, some of them located at three antigenic sites: Ca, Sa, and Sb. Two dominant mutations were detected: P83S (100%) and S203T (85.7%). Glycosylation site analysis revealed the gain of one site at position 162 in 13 sequences. The findings in this study contribute to our understanding of the progress of the influenza A(H1N1)pdm09 virus, since this virus is at the starting point of its evolution in humans.

Influenza RNA viral detection in cerebrospinal fluid by PCR in pediatric patients: first report from Cuba

The average age of the patients was 5 years, ranging between 5 months and 15 years. Clinical records showed fever (90%), cough (85%), and headache (60%) as the most common symptoms. Other symptoms were vomiting and weakness (45%) and diarrhea (37%). The most important finding was the detection of three CSF samples positive for influenza virus – one for type A and two for type C; only the patient with CSF positive for influenza A was positive for the virus in the nasopharyngeal swab. The isolation of influenza A from the nasopharyngeal swab was successful; however the virus could not be isolated from any of the CSF samples. Virus isolation from positive CSF is rarely reported, 8