Benjamin Dewals

Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice

The respiratory tract is continuously exposed to both innocuous airborne antigens and immunostimulatory molecules of microbial origin, such as LPS. At low concentrations, airborne LPS can induce a lung DC-driven Th2 cell response to harmless inhaled antigens, thereby promoting allergic asthma. However, only a small fraction of people exposed to environmental LPS develop allergic asthma. What prevents most people from mounting a lung DC-driven Th2 response upon exposure to LPS is not understood. Here we have shown that lung interstitial macrophages (IMs), a cell population with no previously described in vivo function, prevent induction of a Th2 response in mice challenged with LPS and an experimental harmless airborne antigen. IMs, but not alveolar macrophages, were found to produce high levels of IL-10 and to inhibit LPS-induced maturation and migration of DCs loaded with the experimental harmless airborne antigen in an IL-10-dependent manner. We further demonstrated that specific in vivo elimination of IMs led to overt asthmatic reactions to innocuous airborne antigens inhaled with low doses of LPS. This study has revealed a crucial role for IMs in maintaining immune homeostasis in the respiratory tract and provides an explanation for the paradox that although airborne LPS has the ability to promote the induction of Th2 responses by lung DCs, it does not provoke airway allergy under normal conditions.

Dam break flow computation based on an efficient flux vector splitting

Dam break flow computation is a task of prime interest in the scope of risk analysis processes related to dams and reservoirs. In this paper, a 2D finite volume multiblock flow solver, able to deal with natural topography variation, is presented in detail. The model is based on an efficient Flux Vector Splitting method developed by the authors. A number of validation examples are comprehensively described.

Depth-integrated flow modelling taking into account bottom curvature

Successfully modelling flows over a spillway and on strongly vertically curved bottoms is a challenge for any depth-integrated model. This type of computation requires the use of axes properly inclined along the mean flow direction in the vertical plane and a modelling of curvature effects. The proposed generalized model performs such computations by means of suitable curvilinear coordinates in the vertical plane, leading to a fully integrated approach. This means that the flows in the upstream reservoir, on the spillway, in the stilling basin and in the downstream river reach are all handled in a single simulation. The velocity profile is generalized in comparison with the uniform one usually assumed in the classical shallow water equations. The pressure distribution is modified as a function of the bottom curvature and is thus not purely hydrostatic. Representative test cases, as well as the application of the extended model to the design of a large hydraulic structure in Belgium, lead to satisfactory validation results

Experimental observation of flow characteristics over a Piano Key Weir

The Piano Key Weir is a type of labyrinth weir using overhangs to reduce the footprint of the foundation. These are directly placed on a dam crest. Together with its high discharge capacity for low heads, this geometry makes these weirs interesting in dam rehabilitation. However, the Piano Key Weir is a new weir type, first designed in 2001 and built from 2006 by Electricité de France. Even though experimental studies confirmed its appealing discharge capacities, the flow upstream, over and downstream of this complex structure is still not well known. This research presents experimental test results performed on a 1:10 scale model. The experiments aim at determining the flow features along the weir depending on the upstream head. The flow conditions are characterized in terms of specific discharge, velocity, pressure, water level and streamlines along the weir.

Detailed inundation modelling using high resolution DEMs

Abstract With the availability of high resolution DEMs, relevant and detailed inundation maps may now be routinely computed provided that suitable flow models are available. The full 2D flow model presented in this paper has been used to compute such maps on 800 km of the main rivers of the Walloon Region in Belgium. The use of grid spacing of 1 m, similar to the DEM resolution, enables the accurate prediction of the pattern of flood depth. This is confirmed by several application examples, which also demonstrate the ability of the model to reproduce depth measurements for a wide range of flood discharges without the need for recalibration of the roughness coefficient. The numerical model has been systematically validated by comparison with observations during recent real flood events. It shows a very good agreement with field data, in particular the free surface elevations and inundation extension.

Malignant Catarrhal Fever Induced by Alcelaphine herpesvirus 1 Is Associated with Proliferation of CD8+ T Cells Supporting a Latent Infection

Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be induced in rabbits. The lesions observed are very similar to those described in natural host species. Here, we used the rabbit model and in vivo 5-Bromo-2′-Deoxyuridine (BrdU) incorporation to study WD-MCF pathogenesis. The results obtained can be summarized as follows. (i) AlHV-1 infection induces CD8+ T cell proliferation detectable as early as 15 days post-inoculation. (ii) While the viral load in peripheral blood mononuclear cells remains below the detection level during most of the incubation period, it increases drastically few days before death. At that time, at least 10% of CD8+ cells carry the viral genome; while CD11b+, IgM+ and CD4+ cells do not. (iii) RT-PCR analyses of mononuclear cells isolated from the spleen and the popliteal lymph node of infected rabbits revealed no expression of ORF25 and ORF9, low or no expression of ORF50, and high or no expression of ORF73. Based on these data, we propose a new model for the pathogenesis of WD-MCF. This model relies on proliferation of infected CD8+ cells supporting a predominantly latent infection.

Classification of flow patterns in rectangular shallow reservoirs

This work focuses on the experimental classification of flow patterns in rectangular shallow reservoirs, including symmetric flows without any reattachment point to asymmetric flows with one reattachment point, two reattachment points, or two reattachment points and one detachment point. The median position and the natural variability of the reattachment lengths of asymmetric flows were measured for 40 geometric and hydraulic conditions. The effects of dimensionless flow depth, Froude number, lateral expansion ratio and dimensionless length on the median reattachment lengths were analysed. A number of regression equations were proposed. For “high” dimensionless flow depths and a Froude number of 0.20, a shape parameter was proposed for predicting the transition between symmetric and asymmetric flows. The results of this study are useful knowledge for improving current methods to predict the trapping efficiency and the preferential regions of deposition in reservoirs.

Numerical Investigation of Flow Patterns in Rectangular Shallow Reservoirs

Abstract: In this study, the capability of a two-dimensional shallow-water numerical model to simulate the symmetric and asymmetric flows that can take place in rectangular shallow reservoirs with different lateral expansion ratios and dimensionless lengths is investigated. Numerically, the main difficulty is to properly reproduce the transition between symmetric and asymmetric flows. For a large lateral expansion ratio, the use of two protocols of simulation highlighted a high sensitivity of the simulated flow pattern to the initial condition. Comparison between simulated results and experimental data showed a good agreement for the critical shape parameter (combination of the lateral expansion ratio and the dimensionless length) between symmetric and asymmetric flows. A good agreement was also found for the value of the shorter reattachment length of asymmetric flows. For small lateral expansion ratios, the agreement was not so good. The model was used for even larger lateral expansion ratios in order to numerically extend the experimental dataset. This predictive work showed that the shape parameter, whose expression was only based on experiments carried out for small lateral expansion ratios, was also relevant for larger values. Moreover, the predicted values of the shorter reattachment length were also consistent with a regression only based on experimental results.

Experimental investigation of flow pattern and sediment deposition in rectangular shallow reservoirs

Abstract This paper reports the experimental investigation of flow pattern, preferential regions of deposition and trap efficiency as a function of the length of rectangular shallow reservoirs. Four flow patterns were identified (from longer to shorter reservoirs): an asymmetric flow with two reattachment points, an asymmetric flow with one reattachment point, an unstable flow, and a symmetric flow without any reattachment point. Using dye visualizations, the median value and the temporal variability of the reattachment lengths were precisely measured for the asymmetric flows. For each stable flow, sediment tests with plastic particles were carried out. The regions of deposition on the bed of the reservoir were clearly a function of the flow pattern. The transition from an asymmetric flow pattern to a symmetric flow pattern was responsible for an abrupt decrease of the trap efficiency; a number of regression laws were discussed to take it into account.

An exact Riemann solver and a Godunov scheme for simulating highly transient mixed flows

The current research aims at deriving a one-dimensional numerical model for describing highly transient mixed flows. In particular, this paper focuses on the development and assessment of a unified numerical scheme adapted to describe free-surface flow, pressurized flow and mixed flow (characterized by the simultaneous occurrence of free-surface and pressurized flows). The methodology includes three steps. First, the authors derived a unified mathematical model based on the Preissmann slot model. Second, a first-order explicit finite volume Godunov-type scheme is used to solve the set of equations. Third, the numerical model is assessed by comparison with analytical, experimental and numerical results. The key results of the paper are the development of an original negative Preissmann slot for simulating sub-atmospheric pressurized flow and the derivation of an exact Riemann solver for the Saint-Venant equations coupled with the Preissmann slot.