Benjamin J. Vakoc
Harvard University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Benjamin J. Vakoc.
Nature Medicine | 2009
Benjamin J. Vakoc; Ryan M. Lanning; James Alex Tyrrell; Timothy P. Padera; Lisa A. Bartlett; Triantafyllos Stylianopoulos; Guillermo J. Tearney; Dai Fukumura; Rakesh K. Jain; Brett E. Bouma
Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, crucial aspects of tissue biology. Using unique OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies.
Nature Medicine | 2006
Seok Hyun Yun; Guillermo J. Tearney; Benjamin J. Vakoc; Milen Shishkov; Wang-Yuhl Oh; Adrien E. Desjardins; Melissa J. Suter; Raymond Chan; John A. Evans; Ik-Kyung Jang; Norman S. Nishioka; Johannes F. de Boer; Brett E. Bouma
Comprehensive volumetric microscopy of epithelial, mucosal and endothelial tissues in living human patients would have a profound impact in medicine by enabling diagnostic imaging at the cellular level over large surface areas. Considering the vast area of these tissues with respect to the desired sampling interval, achieving this goal requires rapid sampling. Although noninvasive diagnostic technologies are preferred, many applications could be served by minimally invasive instruments capable of accessing remote locations within the body. We have developed a fiber-optic imaging technique termed optical frequency-domain imaging (OFDI) that satisfies these requirements by rapidly acquiring high-resolution, cross-sectional images through flexible, narrow-diameter catheters. Using a prototype system, we show comprehensive microscopy of esophageal mucosa and of coronary arteries in vivo. Our pilot study results suggest that this technology may be a useful clinical tool for comprehensive diagnostic imaging for epithelial disease and for evaluating coronary pathology and iatrogenic effects.
Optics Express | 2005
Benjamin J. Vakoc; Seok Hyun Yun; J. F. de Boer; Guillermo J. Tearney; Brett E. Bouma
Phase-resolved Doppler optical coherence tomography has been used to image blood flow dynamics in various tissues using both time-domain and spectral-domain optical coherence tomography techniques. In this manuscript, we present phase-resolved Doppler imaging with a high-speed optical frequency domain imaging system. We demonstrate that by correcting for spurious timing-induced phase errors, excellent flow sensitivity can be achieved, limited only by the imaging signal-to-noise ratio. Conventional and Doppler images showing flow in an Intralipid phantom and in human skin are presented. Additionally, we demonstrate the ability of phase-resolved OFDI to measure high flow rates without the deleterious effects of fringe washout.
Jacc-cardiovascular Imaging | 2008
Guillermo J. Tearney; Sergio Waxman; Milen Shishkov; Benjamin J. Vakoc; Melissa J. Suter; Mark I. Freilich; Adrien E. Desjardins; W. Y. Oh; Lisa A. Bartlett; Mireille Rosenberg; Brett E. Bouma
OBJECTIVES We present the first clinical experience with intracoronary optical frequency domain imaging (OFDI) in human patients. BACKGROUND Intracoronary optical coherence tomography (OCT) is a catheter-based optical imaging modality that is capable of providing microscopic (approximately 7-microm axial resolution, approximately 30-microm transverse resolution), cross-sectional images of the coronary wall. Although the use of OCT has shown substantial promise for imaging coronary microstructure, blood attenuates the OCT signal, necessitating prolonged, proximal occlusion to screen long arterial segments. OFDI is a second-generation form of OCT that is capable of acquiring images at much higher frame rates. The increased speed of OFDI enables rapid, 3-dimensional imaging of long coronary segments after a brief, nonocclusive saline purge. METHODS Volumetric OFDI images were obtained in 3 patients after intracoronary stent deployment. Imaging was performed in the left anterior descending and right coronary arteries with the use of a nonocclusive saline purge rates ranging from 3 to 4 ml/s and for purge durations of 3 to 4 s. After imaging, the OFDI datasets were segmented using previously documented criteria and volume rendered. RESULTS Good visualization of the artery wall was obtained in all cases, with clear viewing lengths ranging from 3.0 to 7.0 cm at pullback rates ranging from 5 to 20 mm/s. A diverse range of microscopic features were identified in 2 and 3 dimensions, including thin-capped fibroatheromas, calcium, macrophages, cholesterol crystals, bare stent struts, and stents with neointimal hyperplasia. There were no complications of the OFDI procedure. CONCLUSIONS Our results demonstrate that OFDI is a viable method for imaging the microstructure of long coronary segments in patients. Given its ability to provide microscopic information in a practical manner, this technology may be useful for studying human coronary pathophysiology in vivo and as a clinical tool for guiding the management of coronary artery disease.
Nature Reviews Cancer | 2012
Benjamin J. Vakoc; Dai Fukumura; Rakesh K. Jain; Brett E. Bouma
The past decade has seen dramatic technological advances in the field of optical coherence tomography (OCT) imaging. These advances have driven commercialization and clinical adoption in ophthalmology, cardiology and gastrointestinal cancer screening. Recently, an array of OCT-based imaging tools that have been developed for preclinical intravital cancer imaging applications has yielded exciting new capabilities to probe and to monitor cancer progression and response in vivo. Here, we review these results, forecast the future of OCT for preclinical cancer imaging and discuss its exciting potential to translate to the clinic as a tool for monitoring cancer therapy.
Optics Express | 2008
Wang-Yuhl Oh; Seok Hyun Yun; Benjamin J. Vakoc; Milen Shishkov; Adrien E. Desjardins; Boris Hyle Park; J. F. de Boer; Guillermo J. Tearney; E. Bouma
Polarization sensitive optical coherence tomography (PS-OCT) provides a cross-sectional image of birefringence in biological samples that is complementary in many applications to the standard reflectance-based image. Recent ex vivo studies have demonstrated that birefringence mapping enables the characterization of collagen and smooth muscle concentration and distribution in vascular tissues. Instruments capable of applying these measurements percutaneously in vivo may provide new insights into coronary atherosclerosis and acute myocardial infarction. We have developed a polarization sensitive optical frequency domain imaging (PS-OFDI) system that enables high-speed intravascular birefringence imaging through a fiber-optic catheter. The novel design of this system utilizes frequency multiplexing to simultaneously measure reflectance of two incident polarization states, overcoming concerns regarding temporal variations of the catheter fiber birefringence and spatial variations in the birefringence of the sample. We demonstrate circular cross-sectional birefringence imaging of a human coronary artery ex vivo through a flexible fiber-optic catheter with an A-line rate of 62 kHz and a ranging depth of 6.2 mm.
Gastrointestinal Endoscopy | 2008
Melissa J. Suter; Benjamin J. Vakoc; Patrick Yachimski; Milen Shishkov; Gregory Y. Lauwers; Mari Mino-Kenudson; Brett E. Bouma; Norman S. Nishioka; Guillermo J. Tearney
BACKGROUND Optical coherence tomography (OCT) is a cross-sectional, high-resolution imaging modality that has been shown to accurately differentiate esophageal specialized intestinal metaplasia (SIM) from gastric cardia at the squamocolumnar junction (SCJ) and diagnose high-grade dysplasia and intramucosal carcinoma in patients with SIM. The clinical utility of OCT has been limited, however, by its inability to acquire images over large areas. OBJECTIVE The aim of this study was to use recently developed high-speed OCT technology, termed optical frequency domain imaging (OFDI), and a new balloon-centering catheter (2.5 cm diameter) to demonstrate the feasibility of large area, comprehensive optical microscopy of the entire distal esophagus (approximately 6.0 cm) in patients. DESIGN A pilot feasibility study. SETTING Massachusetts General Hospital. PATIENTS Twelve patients undergoing routine EGD. RESULTS Comprehensive microscopy of the distal esophagus was successfully performed in 10 patients with the OFDI system and balloon catheter. There were no complications resulting from the imaging procedure. Volumetric data sets were acquired in less than 2 minutes. OFDI images at the SCJ showed a variety of microscopic features that were consistent with histopathologic findings, including squamous mucosa, cardia, SIM with and without dysplasia, and esophageal erosion. LIMITATIONS Inability to obtain direct correlation of OFDI data and histopathologic diagnoses. CONCLUSIONS Comprehensive volumetric microscopy of the human distal esophagus was successfully demonstrated with OFDI and a balloon-centering catheter, providing a wealth of detailed information about the structure of the esophageal wall. This technique will support future studies to compare OFDI image information with histopathologic diagnoses.
Cell | 2013
Matija Snuderl; Ana Batista; Nathaniel D. Kirkpatrick; Carmen Ruiz de Almodovar; Lars Riedemann; Elisa C. Walsh; Rachel Anolik; Yuhui Huang; John D. Martin; Walid S. Kamoun; Ellen Knevels; Thomas Schmidt; Christian T. Farrar; Benjamin J. Vakoc; Nishant Mohan; Euiheon Chung; Sylvie Roberge; Teresa Peterson; Carlos Bais; Boryana Zhelyazkova; Stephen Yip; Martin Hasselblatt; Claudia Rossig; Elisabeth Niemeyer; Napoleone Ferrara; Michael Klagsbrun; Dan G. Duda; Dai Fukumura; Lei Xu; Peter Carmeliet
Medulloblastoma is the most common pediatric malignant brain tumor. Although current therapies improve survival, these regimens are highly toxic and are associated with significant morbidity. Here, we report that placental growth factor (PlGF) is expressed in the majority of medulloblastomas, independent of their subtype. Moreover, high expression of PlGF receptor neuropilin 1 (Nrp1) correlates with poor overall survival in patients. We demonstrate that PlGF and Nrp1 are required for the growth and spread of medulloblastoma: PlGF/Nrp1 blockade results in direct antitumor effects in vivo, resulting in medulloblastoma regression, decreased metastasis, and increased mouse survival. We reveal that PlGF is produced in the cerebellar stroma via tumor-derived Sonic hedgehog (Shh) and show that PlGF acts through Nrp1-and not vascular endothelial growth factor receptor 1-to promote tumor cell survival. This critical tumor-stroma interaction-mediated by Shh, PlGF, and Nrp1 across medulloblastoma subtypes-supports the development of therapies targeting PlGF/Nrp1 pathway.
Applied Physics Letters | 2006
Wang-Yuhl Oh; Seok Hyun Yun; Benjamin J. Vakoc; Guillermo J. Tearney; Brett E. Bouma
We demonstrate a linear laser resonator incorporating a semiconductor optical amplifier and scanning filter for high repetition rate, broad wavelength, unidirectional scanning. The laser operates at up to 115kHz repetition rates and demonstrates a tuning-speed-independent power of >30mW. We apply this laser to enable ultrahigh-speed optical frequency domain imaging of the dynamics of laser ablation of biological tissue. The imaging system acquires single longitudinal scans (A-lines) in 8.7μs and complete cross-sectional images comprising 575A-lines at a rate of 200 frames per second.
Optics Letters | 2010
Wang-Yuhl Oh; Benjamin J. Vakoc; Milen Shishkov; Guillermo J. Tearney; Brett E. Bouma
We demonstrate a high-speed wavelength-swept laser with a tuning range of 104 nm (1228-1332 nm) and a repetition rate of 403 kHz. The design of the laser utilizes a high-finesse polygon-based wavelength-scanning filter and a short-length unidirectional ring resonator. Optical frequency domain imaging of the human skin in vivo is presented using this laser, and the system shows sensitivity of higher than 98 dB with single-side ranging depth of 1.7 mm over 4 dB sensitivity roll-off.