Benny Mote

Genetic and genomic basis of antibody response to porcine reproductive and respiratory syndrome (PRRS) in gilts and sows

BackgroundOur recent research showed that antibody response to porcine reproductive and respiratory syndrome (PRRS), measured as sample-to-positive (S/P) ratio, is highly heritable and has a high genetic correlation with reproductive performance during a PRRS outbreak. Two major quantitative trait loci (QTL) on Sus scrofa chromosome 7 (SSC7; QTLMHC and QTL130) accounted for ~40xa0% of the genetic variance for S/P. Objectives of this study were to estimate genetic parameters for PRRS S/P in gilts during acclimation, identify regions associated with S/P, and evaluate the accuracy of genomic prediction of S/P across populations with different prevalences of PRRS and using different single nucleotide polymorphism (SNP) sets.MethodsPhenotypes and high-density SNP genotypes of female pigs from two datasets were used. The outbreak dataset included 607 animals from one multiplier herd, whereas the gilt acclimation (GA) dataset included data on 2364 replacement gilts from seven breeding companies placed on health-challenged farms. Genomic prediction was evaluated using GA for training and validation, and using GA for training and outbreak for validation. Predictions were based on SNPs across the genome (SNPAll), SNPs in one (SNPMHC and SNP130) or both (SNPSSC7) QTL, or SNPs outside the QTL (SNPRest).ResultsHeritability of S/P in the GA dataset increased with the proportion of PRRS-positive animals in the herd (from 0.28 to 0.47). Genomic prediction accuracies ranged from low to moderate. Average accuracies were highest when using only the 269 SNPs in both QTL regions (SNPSSC7, with accuracies of 0.39 and 0.31 for outbreak and GA validation datasets, respectively. Average accuracies for SNPALL, SNPMHC, SNP130, and SNPRest were, respectively, 0.26, 0.39, 0.21, and 0.05 for the outbreak, and 0.28, 0.25, 0.22, and 0.12, for the GA validation datasets.ConclusionsModerate genomic prediction accuracies can be obtained for PRRS antibody response using SNPs located within two major QTL on SSC7, while the rest of the genome showed limited predictive ability. Results were obtained using data from multiple genetic sources and farms, which further strengthens these findings. Further research is needed to validate the use of S/P ratio as an indicator trait for reproductive performance during PRRS outbreaks.

Automated collection of heat stress data in livestock: new technologies and opportunities

© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Transl. Anim. Sci. 2018.2:319–323 doi: 10.1093/tas/txy061

The heritability of pampiniform plexus vessel size and varicocoele in boars

Ultrasonography was used to capture a coronal-sagittal image of the veins of the pampiniform plexus (PP) and the testicular artery of 327 maternal-line boars at approximately 6xa0months of age at the University of Nebraska-Lincoln. Varicocoele was diagnosed by two methods. Method 1 diagnosed varicocoele when the average vessel area on one side of the scrotum was 1.5 times larger than the average vessel area on the other side of the scrotum. Method 2 diagnosed varicocoele when the average vessel area on one side of the scrotum of a boar was 1.5 times larger than the average vessel on the same side of the scrotum of the boars cohorts (same population and year). Varicocoele was diagnosed in 23.17% and 15.1% of boars measured using method 1 and method 2, respectively. Ultrasonography showed to be an effective means to measure PP vessel size in boars and may even allow for earlier detection of varicocoele than by using palpation. Animal models were employed to estimate the heritability for: average area of right PP vessels (0.52), average area of the left PP vessels (0.46), varicocoele presence using method 1 (0.26) and varicocoele presence using method 2 (0.25). These heritability estimates suggest that vessel size and varicocoele could be selected against in breeding programmes to potentially improve boar semen quality.

Objective evaluation of female feet and leg joint conformation at time of selection and post first parity in swine1

Feet and legs of replacement females were objectively evaluated at selection, i.e. approximately 150 days of age (n=319) and post first parity, i.e. any time after weaning of first litter and before 2nd parturition (n=277) to 1) compare feet and leg joint angle ranges between selection and post first parity; 2) identify feet and leg joint angle differences between selection and first three weeks of second gestation; 3) identify feet and leg join angle differences between farms and gestation days during second gestation; and 4) obtain genetic variance components for conformation angles for the two time points measured. Angles for carpal joint (knee), metacarpophalangeal joint (front pastern), metatarsophalangeal joint (rear pastern), tarsal joint (hock), and rear stance were measured using image analysis software. Between selection and post first parity significant differences were observed for all joints measured (P < 0.05). Knee, front and rear pastern angles were less (more flexion), and hock angles were greater (less flexion) as age progressed (P < 0.05), while the rear stance pattern was less (feet further under center) at selection than post first parity (only including measures during first three weeks of second gestation). Only using post first parity leg conformation information, farm was a significant source of variation for front and rear pasterns and rear stance angle measurements (P < 0.05). Knee angle was less (more flexion) (P < 0.05) as gestation age progressed. Heritability estimates were low to moderate (0.04 - 0.35) for all traits measured across time points. Genetic correlations between the same joints at different time points were high (> 0.8) between the front leg joints and low (<0.2) between the rear leg joints. High genetic correlations between time points indicate that the trait can be considered the same at either time point, and low genetic correlations indicate that the trait at different time points should be considered as two separate traits. Minimal change in the front leg suggests conformation traits that remain between selection and post first parity, while larger changes in rear leg indicate that rear leg conformation traits should be evaluated at multiple time periods.

Phenotypic and Genetic Associations of Objectively Evaluated Replacement Female Feet and Leg Joint Conformation at Selection and Post First Parity

Recommended Citation Stock, Joseph D.; Calderón Díaz, Julia A.; Rothschild, Max F.; Mote, Benny E.; and Stalder, Kenneth J. (2018) Phenotypic and Genetic Associations of Objectively Evaluated Replacement Female Feet and Leg Joint Conformation at Selection and Post First Parity, Animal Industry Report: AS 664, ASL R3277. DOI: https://doi.org/10.31274/ans_air-180814-396 Available at: https://lib.dr.iastate.edu/ans_air/vol664/iss1/75

Development of an Objective Feet and Leg Conformation Evaluation Method Using Digital Imagery in Swine

Background: The objectives of this study were to create an objective measurement method of joint angles for knee, hock, front and rear pasterns and a rear stance position in swine using digital imaging technology and to assess the repeatability of the objective measurement process. nMethods and Findings: Forty-five multiparous sows (average parity 6.7 ± 2.5; parity range 5 to 14) from two commercial farms (n=21 farm 1 and n=24 farm 2) were used. Sows were moved to a pen where digital images of the profile and rear stance were captured. On average, 5.2 (± 2.6) profile and 2.6 (± 1.0) rear stance high quality images were used per sow. A joint angle measuring system was devised to collect angle measurements on the four feet and leg joints previously mentioned and the rear stance. Joint measurements were analyzed using repeated measure mixed model methods, including farm and parity (as 5, 6, and 7+) as fixed effects. Intraclass correlation coefficients were calculated to evaluate process repeatability. Joint angle measurement repeatability ranged from 0.63 to 0.82. Lowest and highest repeatabilities were observed for the front pastern and hock angle measurements, respectively. No significant farm or parity differences were observed for joint angles measured except for the knee angle between farms (P<0.05) and the hock angle between sows’ parities 5 and 6 and parity 7+ (P<0.05). nConclusions: Feet and leg conformation evaluation using digital images could be successfully used as an objective tool to aide in selection of replacement gilts. This could have a beneficial impact on sow longevity and farm productivity and profitability.

Tracking of group-housed pigs using multi-ellipsoid expectation maximisation

Maintaining the health and well-being of animals is critical to the efficiency and profitability of livestock operations. However, it can be difficult to monitor the health of animals in large group-housed settings without the assistance of technology. This study presents a system that uses depth images to continuously track individual pigs in a group-housed environment. It is an alternative to traditional manual observation used by both researchers and producers for the analysis of animal activities and behaviours. The tracking method used by the system exploits the consistent shape and fixed number of the targets in the environment by applying expectation maximisation as a policy for fitting an ellipsoid to each target. Results demonstrate that the system can maintain the correct positions and orientations of 15 group-housed pigs for an average of 19.7u2005min between failure events.