Bernardo Pace

Effect of cooking methods on antioxidant activity and nitrate content of selected wild Mediterranean plants

Abstract Wild edible plants (WEP), traditionally consumed in the Mediterranean diet, are considered a rich source of natural antioxidants but can also accumulate significant amount of nitrates. Most WEP are cooked before consumption, therefore, a study was conducted to evaluate the effects of boiling, steaming and microwave cooking processes on the total antioxidant activity (TAA) and nitrate content of eight common WEP. Boiling caused the highest losses of TAA, resulting in a reduction of the TAA on dry weight (DW) basis ranging from 5.5% in Beta vulgaris up to 100% in Urtica dioica. Steaming and microwaving produced the highest increase of TAA on DW basis in Helminthotheca echioides (249.7%) and Taraxacum officinale (60.7%). Boiling caused the highest reduction of nitrate content in all species excluding Asparagus acutifolius that maintained almost unvaried its already low nitrate content. These results suggest that cooking has not always negative effect on product quality, since in certain cases, it may even enhance the nutritional value of WEP by increasing their TAA and reducing the nitrate content.

Postharvest evaluation of soilless‐grown table grape during storage in modified atmosphere

BACKGROUND Soilless growth systems, developed mainly for vegetables and ornamental crops, have also been used recently as an alternative to soil culture for table grape in order to achieve optimal production performance. In this study, sensory, physical and chemical parameters were analysed in table grapes obtained from soil and soilless growth systems at harvest and during storage in air or modified atmosphere. RESULTS At harvest, soilless-grown berries were 30% firmer than those grown in soil. Moreover, they showed 60% higher antioxidant activity and total phenol content than soil-grown fruits. Modified atmosphere storage resulted in a better quality of table grapes compared with those stored in air. Furthermore, soilless growth was more suitable than soil growth for preserving visual quality and controlling rachis browning and weight loss. CONCLUSION Since the soilless system produces berries that are cleaner and of higher quality than those grown in soil, the implementation of soilless growth for the production of health-promoting and convenience fruits is suggested.

Non-destructive evaluation of quality and ammonia content in whole and fresh-cut lettuce by computer vision system

The paper describes the developed hardware and software components of a computer vision system that extracts colour parameters from calibrated colour images and identifies non-destructively the different quality levels exhibited by lettuce (either whole or fresh-cut) during storage. Several colour parameters extracted by computer vision system have been evaluated to characterize the product quality levels. Among these, brown on total and brown on white proved to achieve a good identification of the different quality levels on whole and fresh-cut lettuce (P-value<0.0001). In particular, these two parameters were able to discriminate three levels: very good or good products (quality levels from 5 to 4), samples at the limit of marketability (quality level of 3) and waste items (quality levels from 2 to 1). Quality levels were also chemically and physically characterized. Among the parameters analysed, ammonia content proved to discriminate the marketable samples from the waste in both products typologies (either fresh-cut or whole); even the two classes of waste were well discriminated by ammonia content (P-value<0.0001). A function that infers quality levels from the extracted colour parameters has been identified using a multi-regression model (R2=0.77). Multi-regression also identified a function that predicts the level of ammonia (an indicator of senescence) in the iceberg lettuce from a colour parameter provided by the computer vision system (R2=0.73), allowing a non-destructive evaluation of a chemical parameter that is particularly useful for the objective assessment of lettuce quality. The developed computer vision system offers flexible and simple non-destructive tool that can be employed in the food processing industry to monitor the quality and shelf life of whole and fresh-cut lettuce in a reliable, objective and quantitative way.

Relationship between Quality Parameters and the Overall Appearance in Lettuce during Storage

Fresh-cut and whole iceberg lettuce were stored in refrigerated condition and the main sensory (overall appearance: OA, browning index: BI), physical (colour variation: E*), chemical (respiration activity: RA, ammonia content: A) and biochemical (total chlorophyll: TC, antioxidant activity: AA, total phenols: TP, o-quinones: o-q, polyphenol oxidase: PPO, phenylalanine ammonia-lyase: PAL and peroxidase: POD) traits were followed during the trial. Significant relationships among these parameters were assessed by principal component analysis (PCA) in order to find the main traits related to OA, in fresh-cut lettuce. Results from PCA showed that OA was inversely correlated with BI (r=-1.00), E* (r= -0.98), A (r= -0.94), AA (r= -0.86), TP (r= -0.82), o-q (r= -0.89), and PAL (r= -0.80). Moreover, OA was positively related with RA (r= 1.00) and PPO activity (r= 0.96). Ammonia resulted significantly correlated with the main sensory, physical, chemical and biochemical parameters. Thus, this parameter, with E* could be used as indicator of the product quality and acceptability in control quality procedures. In conclusion, rating scale used to estimate OA, resulted a valid method to evaluate the iceberg lettuce quality, since it showed significant relationships with the main analytical parameters.

Changes in visual quality, physiological and biochemical parameters assessed during the postharvest storage at chilling or non-chilling temperatures of three sweet basil (Ocimum basilicum L.) cultivars

Leaves of three different sweet basil (Ocimum basilicum L.) cultivars (Italico a foglia larga, Cammeo, and Italiano classico) packed in macro-perforated polyethylene bags were stored at chilling (4°C) or non-chilling temperature (12°C) for 9days. During storage, visual quality, physiological (respiration rate, ethylene production, ammonium content) and chemical (antioxidant activity, total polyphenols and polyphenol profile) parameters were measured. Detached leaves stored at chilling temperature showed visual symptoms related to chilling injury, while ethylene production and ammonium content resulted associated to cultivar sensibility to damage at low temperature. Storage at 4°C caused a depletion in polyphenols content and antioxidant capability, which was preserved at 12°C. Regarding the polyphenols profile, stressful storage conditions did not enhance the phenolic metabolism. However, leaves stored at 12°C did not loss a significant amount of metabolites respect to fresh leaves, suggesting the possibility to extend the storability after the expiration date, for a possible recovery of bioactive compounds.