Bernd Vogt

Fluorescence Polarisation Immunoassay

Since the introduction of radio immunoassay techniques [1], many different assay principles based on antibodies as specifiers have become routine tools in clinical diagnostics. Heterogeneous immunoassays, e.g. enzyme linked immunosorbant assays (ELISA) [2], require a series of time consuming incubation and wash steps, but they are characterized by a high sensitivity with a detection limit of 10−13 moll−1 and even lower.