Bernhard Schnetger

Microbial hitchhikers on intercontinental dust: catching a lift in Chad.

Ancient mariners knew that dust whipped up from deserts by strong winds travelled long distances, including over oceans. Satellite remote sensing revealed major dust sources across the Sahara. Indeed, the Bodélé Depression in the Republic of Chad has been called the dustiest place on earth. We analysed desert sand from various locations in Chad and dust that had blown to the Cape Verde Islands. High throughput sequencing techniques combined with classical microbiological methods showed that the samples contained a large variety of microbes well adapted to the harsh desert conditions. The most abundant bacterial groupings in four different phyla included: (a) Firmicutes—Bacillaceae, (b) Actinobacteria—Geodermatophilaceae, Nocardiodaceae and Solirubrobacteraceae, (c) Proteobacteria—Oxalobacteraceae, Rhizobiales and Sphingomonadaceae, and (d) Bacteroidetes—Cytophagaceae. Ascomycota was the overwhelmingly dominant fungal group followed by Basidiomycota and traces of Chytridiomycota, Microsporidia and Glomeromycota. Two freshwater algae (Trebouxiophyceae) were isolated. Most predominant taxa are widely distributed land inhabitants that are common in soil and on the surfaces of plants. Examples include Bradyrhizobium spp. that nodulate and fix nitrogen in Acacia species, the predominant trees of the Sahara as well as Herbaspirillum (Oxalobacteraceae), a group of chemoorganotrophic free-living soil inhabitants that fix nitrogen in association with Gramineae roots. Few pathogenic strains were found, suggesting that African dust is not a large threat to public health.

Consistent assessment of trace metal contamination in surface sediments and suspended particulate matter: A case study from the Jade Bay in NW Germany

Recently, within the framework of European directives, the importance of marine monitoring programs has increased. In this study, a dense sampling grid was applied for a detailed assessment of the metal contents of surface sediments and suspended particulate matter from the Jade Bay, one of the tidal basins in the southern North Sea. The local lithogenic background was defined and compared with average shale, a common reference material. Based on the calculated non-lithogenic fraction and a cluster analysis, the metals are distributed in two groups: (i) elements of mainly natural origin (Co, Cr, and a major portion of Cd) and (ii) elements associated with anthropogenic activity (As, Cd, Cu, Ni, Pb, Sn, and Zn). However, even the metals of the second group are enriched by at most a factor of two relative to the local background, suggesting minimal anthropogenic impact. Spatial distribution maps show that the harbor area of Wilhelmshaven may be a particularly important source of metal.

A geochemical record of late Holocene palaeoenvironmental changes at King George Island (maritime Antarctica)

Abstract During RV Polarstern cruise ANT-XXIII/4 in 2006, a gravity core (PS 69/335-2) and a giant box core (PS 69/335-1) were retrieved from Maxwell Bay off King George Island (KGI). Comprehensive geochemical (bulk parameters, quantitative XRF, Inductively Coupled Plasma Mass Spectrometry) and radiometric dating analyses (14C, 210Pb) were performed on both cores. A comparison with geochemical data from local bedrock demonstrates a mostly detrital origin for the sediments, but also points to an overprint from changing bioproductivity in the overlying water column in addition to early diagenetic processes. Furthermore, ten tephra layers that were most probably derived from volcanic activity on Deception Island were identified. Variations in the vertical distribution of selected elements in Maxwell Bay sediments further indicate a shift in source rock provenance as a result of changing glacier extents during the past c. 1750 years that may be linked to the Little Ice Age and the Medieval Warm Period. Whereas no evidence for a significant increase in chemical weathering rates was found, 210Pb data revealed that mass accumulation rates in Maxwell Bay have almost tripled since the 1940s (0.66 g cm-2 yr-1 in ad 2006), which is probably linked to rapid glacier retreat in this region due to recent warming.

Geochemistry of sediments from the connection between the western and the eastern Mediterranean Sea (Strait of Sicily, ODP Site 963)

Abstract A geochemical and stable isotope geochemical study was carried out on sediments and pore waters from the Strait of Sicily at the connection between the western and the eastern Mediterranean Sea (ODP Site 963). Organic-matter-rich layers (ORLs), which are proposed to be time-equivalent to eastern Mediterranean sapropels, only occur in sediments older than 550 kyr, most likely because of a strong dilution of late Pleistocene and Holocene sediments by terrigenous material. The ORLs have total organic carbon (TOC) contents of up to 1.6% and are enriched in pyrite and trace metals. TOC/pyrite ratios indicate the at least temporary presence of an anoxic water column during sediment formation, which is in agreement with the observed sulfur isotope discrimination. These conditions induced the enrichment in sulfide-forming and redox-sensitive trace elements. Trace metal enrichment is lower than at other sites in the Mediterranean Sea, which may be attributed to a less reducing or a less stable anoxic environment that was less favorable for the accumulation of redox-sensitive trace elements and organic matter. However, during sapropel events sedimentary conditions in the Strait of Sicily, based on manganese and barium geochemistry, must have been largely similar to those at other sites in the Mediterranean Sea. According to elemental, Rock-Eval, and stable carbon isotope analyses the organic matter in the ORLs is mainly of marine origin, which appears to have been overprinted significantly during diagenesis. A significant part of the organic matter in sediments from ODP Site 963 may also originate from uplifted land sections eroded during weathering and may primarily also be of marine origin. The results of pore water analyses illustrate the presence of an active deep biosphere in the sediments of Site 963, reflected for example by sulfate, methane and iodine concentrations. A brine, probably originating from underlying Messinian evaporites, was detected, but is only of minor influence.

Anaerobic sulfur oxidation in the absence of nitrate dominates microbial chemoautotrophy beneath the pelagic chemocline of the eastern Gotland Basin, Baltic Sea

Oxic-anoxic interfaces harbor significant numbers and activity of chemolithoautotrophic microorganisms, known to oxidize reduced sulfur or nitrogen species. However, measurements of in situ distribution of bulk carbon dioxide (CO(2)) assimilation rates and active autotrophic microorganisms have challenged the common concept that aerobic and denitrifying sulfur oxidizers are the predominant autotrophs in pelagic oxic-anoxic interfaces. Here, we provide a comparative investigation of nutrient, sulfur, and manganese chemistry, microbial biomass distribution, as well as CO(2) fixation at the pelagic redoxcline of the eastern Gotland Basin, Baltic Sea. Opposing gradients of oxygen, nitrate, and sulfide approached the detection limits at the chemocline at 204 m water depth. No overlap of oxygen or nitrate with sulfide was observed, whereas particulate manganese was detected down to 220 m. More than 70% of the bulk dark CO(2) assimilation, totaling 9.3 mmol C m(-2) day(-1), was found in the absence of oxygen, nitrite, and nitrate and could not be stimulated by their addition. Maximum fixation rates of up to 1.1 mumol C L(-1) day(-1) were surprisingly susceptible to altered redox potential or sulfide concentration. These results suggest that novel redox-sensitive pathways of microbial sulfide oxidation could account for a significant fraction of chemolithoautotrophic growth beneath pelagic chemoclines. A mechanism of coupled activity of sulfur-oxidizing and sulfur-reducing microorganisms is proposed.

Establishment of euxinic conditions in the Holocene Black Sea

The paleoenvironmental evolution of the Black Sea is closely linked to the ingression of Mediterranean seawater over the Bosporus sill after the Last Glacial Maximum. We have reconstructed the temporal and spatial development of the Black Sea suboxic chemocline, which divides oxic surface water from anoxic, sulfi dic (euxinic) deep water. By combining high-resolution geochemical records of bulk parameters (carbonate, total organic carbon, sulfur), trace metals (Cu, Mo, V), and an isotopic proxy (δ 56 Fe) from seven sediment cores in the Black Sea, we generated a single composite geochemical core log that serves as a reference archive for the entire basin. Our proxy records refl ect the changing depositional and redox conditions of the Black Sea and permit us to estimate the infl ow budget of Mediterranean seawater throughout the Holocene. Our data indicate a gradual rise of the chemocline until ca. 5.3 ka, when suboxic waters fl ooded the shelf for the fi rst time. Trace metal and isotopic inventories document one major descent of the chemocline since the onset of brackish/ marine conditions before the present stable situation was established.

Stable sulfur isotopes indicate net sulfate reduction in near-surface sediments of the deep Arabian Sea

Depth profiles of the stable sulfur isotopic composition of dissolved sulfate in near-surface sediments were measured at five stations of the deep Arabian Sea between 1918 and 4426 m water depth (WAST, WAST-Top, CAST, SAST, NAST), sampled in April 1997. The results clearly indicate that net microbial sulfate reduction took place in the sediments at stations WAST and NAST below about 12 cm depth. Sulfate reduction at WAST was more pronounced compared to station NAST, most likely due to higher organic carbon content in turbiditic sediments. No net sulfate reduction took place within the upper 10 cm of the surface sediments at all stations, and no significant isotopic indication for sulfate reduction was found down to 30 cm bsf at station SAST. Results are in accordance with accumulation of reduced isotopically light sulfur species below about 6 cm bsf at station WAST. It is concluded that the sulfur isotopic composition of remaining sulfate is more sensitive to net sulfate reduction than the [SO4]/[Cl] ratio. The sulfur isotopic composition of a vertical profile for dissolved sulfate through the water column at station WAST was essentially constant (250–4047 m: Full-size image (<1 K)‰ vs. V-CDT n=8). A similar constancy (20–4565 m water depth Full-size image (<1 K)‰ vs. V-CDT n=15) was found for the station BIOTRANS in the northeastern Atlantic (47°11′ 19°33W), indicating that the oxygen minimum zone in the Arabian Sea has no influence on the sulfur isotopic composition of dissolved sulfate.

Are Iron-Phosphate Minerals a Sink for Phosphorus in Anoxic Black Sea Sediments?

Phosphorus (P) is a key nutrient for marine organisms. The only long-term removal pathway for P in the marine realm is burial in sediments. Iron (Fe) bound P accounts for a significant proportion of this burial at the global scale. In sediments underlying anoxic bottom waters, burial of Fe-bound P is generally assumed to be negligible because of reductive dissolution of Fe(III) (oxyhydr)oxides and release of the associated P. However, recent work suggests that Fe-bound P is an important burial phase in euxinic (i.e. anoxic and sulfidic) basin sediments in the Baltic Sea. In this study, we investigate the role of Fe-bound P as a potential sink for P in Black Sea sediments overlain by oxic and euxinic bottom waters. Sequential P extractions performed on sediments from six multicores along two shelf-to-basin transects provide evidence for the burial of Fe-bound P at all sites, including those in the euxinic deep basin. In the latter sediments, Fe-bound P accounts for more than 20% of the total sedimentary P pool. We suggest that this P is present in the form of reduced Fe-P minerals. We hypothesize that these minerals may be formed as inclusions in sulfur-disproportionating Deltaproteobacteria. Further research is required to elucidate the exact mineral form and formation mechanism of this P burial phase, as well as its role as a sink for P in sulfide-rich marine sediments.

A fast method for the simultaneous determination of 230Th, 234U and 235U with isotope dilution sector field ICP-MS

A double focussing sector field ICP-MS with its variable resolution capability was used for the simultaneous measurement of Th and U isotopes to calculate excess 230Th and total U content in marine sediments. The necessary separation and concentration of Th and U from major and minor elements in the sediment samples was achieved by acid digestion followed by anion exchange. ICP-MS measurements were performed using a resolution of R = 4430 resulting in an abundance sensitivity of 230/232 = 5 × 10–7 which is small enough for the quantification of sediments with 230Th/232Th ratios as low as 10–6. Using a microconcentric nebulizer (MCN-100), the lowest sample volume required for a single measurement was less than 200 µl. Analysis time was 80 s per sample. 229Th and 236U were used for quantification and as yield tracers. The overall uncertainty introduced by the entire separation procedure and the ICP-MS analyses was about 5% (95% confidence interval). The precision of each individual measurement was within 3% (2s).

Quantifying K, U, and Th contents of marine sediments using shipboard natural gamma radiation spectra measured on DV JOIDES Resolution

During International Ocean Discovery Program (IODP) expeditions, shipboard-generated data provide the first insights into the cored sequences. The natural gamma radiation (NGR) of the recovered material, for example, is routinely measured on the ocean drilling research vessel DV JOIDES Resolution. At present, only total NGR counts are readily available as shipboard data, although full NGR spectra (counts as a function of gamma-ray energy level) are produced and archived. These spectra contain unexploited information, as one can estimate the sedimentary contents of potassium (K), thorium (Th), and uranium (U) from the characteristic gamma-ray energies of isotopes in the ^(40)K, ^(232)Th, and ^(238)U radioactive decay series. Dunlea et al. [2013] quantified K, Th and U contents in sediment from the South Pacific Gyre by integrating counts over specific energy levels of the NGR spectrum. However, the algorithm used in their study is unavailable to the wider scientific community due to commercial proprietary reasons. Here, we present a new MATLAB algorithm for the quantification of NGR spectra that is transparent and accessible to future NGR users. We demonstrate the algorithms performance by comparing its results to shore-based inductively coupled plasma-mass spectrometry (ICP-MS), inductively coupled plasma-emission spectrometry (ICP-ES), and quantitative wavelength-dispersive X-ray fluorescence (XRF) analyses. Samples for these comparisons come from eleven sites (U1341, U1343, U1366-U1369, U1414, U1428-U1430, U1463) cored in two oceans during five expeditions. In short, our algorithm rapidly produces detailed high-quality information on sediment properties during IODP expeditions at no extra cost.