Bert L. Bishop

Oral toxicity of essential oils and organic acids fed to honey bees (Apis mellifera)

Summary Natural plant products have been studied for potential use as in-hive fumigants for suppression of parasitic mites and other pests. A more direct application through direct feeding of bees would avoid problems with fumigant volatility in cold climates and provide a more systemic route of exposure for the target pest. However, there must be a balance between toxicity to hive pests and toxicity (safety) to the bees. We focused on adult bee toxicity when testing ten products: cineole, clove oil, formic acid, marjoram oil, menthol, oregano oil, oxalic acid, sage oil, thymol, and wintergreen. Each product was tested at several concentrations in a sugar syrup fed to bees over several days, and dead bees were counted daily. Oxalic acid was the most toxic of the products tested. Menthol and cineole had mortality levels no different from controls fed plain syrup after 8 days of treatment. At 14 days of treatment, wintergreen was the least toxic, but neither menthol nor cineole were a part of the testing that went to 14 days. Our results indicate that the tested products could all be used safely for treating bees orally if dose is carefully managed in the hive.

Nitrogen and Carbon accretion on Ohio coal minesoils: Influence of soil-forming factors☆

Abstract Plantations of white pine (Pinus strobus L.), tuliptree (Liriodendron tulipifera L.), white ash (Fraxinus americana L.) and black locust (Robinia pseudoacacia L.), established in 1946 on ungraded calcareous, neutral or acid coal spoils, were sampled in 1976 for weight, carbon and nitrogen content of the forest floor, and for total N concentration in 4 depth increments of the underlying mineral soil. Minesoil characteristics were related to the following soil-forming factors: parent material (overburden), organisms (tree species) and relief (aspect and slope position). Forest floor weight was high under white pine on all parent materials and under black locust on acid parent material. Nitrogen content of the forest floor was highest on acid minesoil, as was total weight of N in the forest floor under all species except tuliptree. Although concentration of N in the forest floor was highest under black locust, total weight of forest floor N under pine exceeded that under locust on calcareous and neutral minesoils because of the much greater weight of forest floor under pine. C/N ration of forest floor was lowest under locust and on the acid minesoil. Total N concentration was highest at all depths in soils that formed under black locust: rate of decrease in total N concentration between the 0–5 and 5–15 cm depth increments was also greatest under black locust. Soils formed on calcareous parent material had higher total N concentrations at all depths than those formed on neutral or acid parent materials. We estimate that concentration of total N in the 0–5 cm horizon of calcareous minesoils has increased from 0.04 to 0.23% in the 30 years since tree establishment. The corresponding increase in the acid minesoils has been from 0.03 to 0.18%. Our results show that accretion of carbon and nitrogen on minesoils will be influenced both by overburden strata placed at the surface as parent material of the new soil and by tree species chosen for planting.

Assessment of single-nozzle patternation and extrapolation to moving booms

The extrapolation from measurements of static deposition patterns produced by single nozzles to multi-nozzle booms was investigated for a wide range of agricultural nozzles, using a non-intrusive patternator. The spray deposit pattern was determined for seven nozzle types for single-nozzle pattern or on a three-nozzle boom at 0.198 km h−1 and for one nozzle (an XR8003VS) at a typical field speed (6.4 km h−1) in a track-room. A method for statistically separating coefficients of variation (CV) for the patterns was derived. For low throughput nozzles, there were statistically significant differences between computer simulations derived from single-nozzle patterns and the patterns produced by multiple-nozzle booms. For the XR8003VS, there were no significant differences between the computer simulation, the statistically derived pattern, or the pattern produced at 6.4 km h−1. The spray deposit patterns produced by the various nozzle combinations were examined with respect to biological efficacy using theoretical dose-response curves covering a wide range of dose responses. A CV of < 10% for a swath pattern can be biologically efficient, but 4.5% of the spray is wasted in overdosing. To compensate for uneven application, increasing dose was shown to be an inefficient means of achieving the biological result desired.

Effect of Insecticides on Tiphia vernalis (Hymenoptera: Tiphiidae) Oviposition and Survival of Progeny to Cocoon Stage When Parasitizing Popillia japonica (Coleoptera: Scarabaeidae) Larvae

Abstract The effect of insecticides on oviposition of Tiphia vernalis Rohwer and subsequent survival of parasitoid progeny to the cocoon stage was determined in the laboratory by using larval Japanese beetle, Popillia japonica Newman, as the host. Insecticides tested were imidacloprid, thiamethoxam, halofenozide, chlorpyrifos, and carbaryl at labeled rates. Female T. vernalis were allowed 2 d to parasitize P. japonica larvae after the parasitoids had received a 4-d exposure to insecticide-treated soil. Another group of female T. vernalis were allowed 2 d to parasitize P. japonica larvae that had been exposed to insecticide-treated soil for 3–4 d. Percentage of parasitism of P. japonica larvae in these trials after exposure of adult parasitoids to carbaryl, chlorpyrifos, halofenozide, or imidacloprid-treated soil (23.3–50.0%) or adult parasitoids to chlorpyrifos, halofenozide, or imidacloprid-treated grubs (33.0–56.7%) was not negatively affected relative to the control treatment (21.7–54.2%). A third group of adult T. vernalis and P. japonica larvae were simultaneously exposed to chlorpyrifos or carbaryl treatments. Percentage parasitism in these trials was lower for T. vernalis adults exposed to the chlorpyrifos and carbaryl (15.0–25.0%) relative to the control (57.5–62.5%) with the exception of one trial with carbaryl (40.0%). However, exposure of the parasitoid and P. japonica to chlorpyrifos 0.5×, carbaryl 0.5×, imidacloprid, halofenozide, or thiamethoxam in several trials resulted in parasitism that was equivalent or greater than (45.0–80.0%) the untreated control (57.5–62.5%). Japanese beetle larval mortality in these trials was greater in the insecticide and parasitoid combination (97.5–100.0%) than with insecticides alone (45.0–100.0%). Percentage of survival of T. vernalis progeny to the cocoon stage was not negatively affected by a 4-d adult parasitoid exposure to carbaryl and chlorpyrifos treated soil (11.7–16.7% versus 18.3% control) or a 2-d exposure to P. japonica-treated larvae (16.7–18.3% versus 28.3% control). However, simultaneous exposure of T. vernalis progeny and P. japonica larvae to chlorpyrifos- and carbaryl-treated soil resulted in no parasitoids surviving to the cocoon stage. Between neonicotinoids, thiamethoxam had more adverse impact on percentage parasitism (52.5%) and survival to the cocoon stage (10.0%) than imidacloprid (80.0 and 32.5%, respectively). Results of this study indicate soil incorporation of imidacloprid and halofenozide had minimal effect on the number of P. japonica larvae parasitized by T. vernalis or survival of T. vernalis progeny to the cocoon stage; therefore, they are more suitable for use with T. vernalis. In contrast, chlorpyrifos, carbaryl, and thiamethoxam lowered the number of T. vernalis progeny surviving to the cocoon stage, and carbaryl and chlorpyrifos reduced the number of P. japonica larvae parasitized. The soil incorporation of insecticides is discussed as one explanation for the minimal effects of some insecticides on T. vernalis.

Chlorpyrifos Immersion to Eliminate Third Instars of Japanese Beetle (Coleoptera: Scarabaeidae) in Balled and Burlapped Trees and Subsequent Treatment Effects on Red Maple

Abstract This study examined chlorpyrifos immersion of balled and burlapped (B&B) nursery trees for elimination of third instars of Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), and for phytotoxicity on red maple, Acer rubrum L. Trees were harvested as 45- and 60-cm-diameter B&B and immersed in chlorpyrifos at U.S. Domestic Japanese Beetle Harmonization Plan rate (0.24 kg active ingredient [AI]/100 liters) or lower rates of 0.015, 0.03, 0.06, and 0.12 kg (AI)/100 liters. The 0.03, 0.06, and 0.24 kg (AI) rates provided 100% control of Japanese beetle grubs in both 45- and 60-cm B&B. The 0.015 and 0.12 kg (AI) chlorpyrifos rates were 100% effective in three tests. However, in another test, 0.015 and 0.12 kg (AI) chlorpyrifos treatments had four (93% control) and one (98% control) grubs recovered, respectively. Root ball soils consisted of loam, silt loam, or clay loam texture classifications. Trunk diameter and internode growth of red maple harvested as 45-cm B&B decreased linearly with increasing chlorpyrifos dip rate during the first year, but effects were unapparent in the second year. Chlorpyrifos rates had no measurable impact on growth of red maples harvested as 60-cm B&B. No visual phytotoxicity symptoms were detected for chlorpyrifos rate or root ball size treatments. In conclusion, results support lowering the U.S. Domestic Japanese Beetle Harmonization Plan chlorpyrifos dip rate for category 2 states to at least 0.03 kg (AI) for B&B diameters ≤60 cm. Chlorpyrifos rates <0.24 kg (AI) will lower cost, reduce worker exposure, and lessen potential environmental contamination.

Surface-applied insecticide treatments for quarantine control of Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), larvae in field-grown nursery plants.

BACKGROUND Japanese beetles, Popillia japonica Newman, are a quarantine challenge for nursery shipments from infested to non-infested states. Marathon (imidacloprid) and Discus (imidacloprid + cyfluthrin) are approved preharvest nursery treatments (US Domestic Japanese Beetle Harmonization Plan; DJHP). This study evaluated approved and non-approved (acephate, carbaryl, clothianidin, dinotefuran, halofenozide, thiamethoxam, trichlorfon) preharvest treatments, optimal rates (labeled 1x, 0.3-0.75 x or 2-3 x) and optimal timings (June, July, August and September) to control early-instar (grubs) P. japonica in field nurseries. RESULTS Most insecticides effectively reduced grub densities, except for acephate, carbaryl and trichlorfon. Clothianidin, thiamethoxam and halofenozide provided grub control equivalent to DJHP standards during most years. Across all test years and timings, percentage grub reductions were: Marathon (1x: 59.2-100; 3 x: 78.9-100), Discus (1x: 60.7-100), clothianidin (1x: 96.1-100; 3 x: 97.4-100), thiamethoxam (1x: 75.0-100; 3 x: 80.0-100), halofenozide (1x: 70.0-100; 3 x: 90.0-100) and dinotefuran (1x: 13.2-88.2; 3 x: 71.1-93.4). CONCLUSIONS July application was more consistent and effective than other timings, and higher rates (2x and 3 x) did not generally improve grub control. Overall, clothianidin, thiamethoxam and halofenozide (and dinotefuran applied in August) were equivalent to current DJHP standards. These insecticides may be suitable for DJHP Category 2 states, potentially lowering grower costs.

Drench Treatments for Management of Larval Japanese Beetle (Coleoptera: Scarabaeidae) in Field-Grown Balled and Burlapped Nursery Plants

Abstract Insecticide drenches were applied to postharvest field-grown nursery plants harvested as 60-cm-diameter balled and burlapped (B&B) root balls for controlling third instars of Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae). Bifenthrin, chlorpyrifos, lambda-cyhalothrin, and thiamethoxam were drench-applied in fall and spring tests at volumes of runoff (1×; ≈2.57 liters per drench per root ball) or twice runoff (2×). Tests also examined consecutive drenches (two, four, or six) and B&B rotation between drenches. Fall-applied drenches did not meet the Domestic Japanese Beetle Harmonization Plan (DJHP) standards of ≤1 grub and ranged from 0 to 90% control. However, most fall-applied drenches significantly reduced grub numbers relative to the untreated root balls. Spring-applied drenches were more effective than fall drenches: chlorpyrifos treatments gave 94–100% control, whereas other spring-applied treatments were less consistent, including thiamethoxam (83–100% control) and bifenthrin (61–100% control). Lambda-cyhalothrin was not effective. A higher drench volume (2×) did not significantly improve treatment efficacy; however, grub numbers decreased as the number of drenches increased for fall-applied chlorpyrifos and thiamethoxam and spring-applied bifenthrin. Rotation of root balls significantly reduced grub numbers compared with nonrotated treatments for fall-applied chlorpyrifos (six drenches) and bifenthrin (two or six drenches), but these treatments did not meet DJHP standards. The study indicates chlorpyrifos, bifenthrin, and thiamethoxam drenches can control Japanese beetle in the spring and may provide a new postharvest option to certify B&B plants for Japanese beetle.