Berthold Szperalski

Industrial production of monoclonal antibodies and therapeutic proteins by dialysis fermentation

A novel and powerful fermentation method is reported for the large-scale growth of mammalian cells and their secreted products. The system described illustrates many of the advantages of conventional batch fermentation processes but in addition has been shown to yield cell densities in excess of 1×107 cells/ml with concomitant increase in product concentration.

Simulation of Cell Cycle Dependent Growth and Metabolism in Animal Cell Fermentation

Abstract Growth and metabolism of mouse-mouse hybridoma cell lines were simulated with the help of a structured model based on the segregated fractions of cell cycle. This model uses constant metabolic rates for the different phases of cell cycle. The change of specific rates from the whole cell population during cultivation is modelled by the shift between cycling and resting phases, which is dependent from the culture conditions. Simulation of the cell cycle dependent growth was performed and compared for batch, dialysis, continuous and split batch fermentation processes.