Betty J. Hargis

Anaphylactic shock in the pertussis-vaccinated mouse☆

Abstract 1.1. It has been confirmed that H. pertussis vaccination of white mice produces a fiftyfold increase in histamine sensitivity. 2.2. Brucella abortus vaccine enhanced histamine sensitivity to a much less but significant degree. 3.3. Mice immunized by an intraperitoneal injection of a mixture of H. pertussis vaccine and horse serum become markedly anaphylactically sensitive to the serum. Brucella abortus vaccine has a somewhat similar effect on anaphylaxis. 4.4. The results of further experimentation seem to substantiate the impression that histamine plays a minor role in anaphylaxis in the mouse even when the mouse has been made markedly histamine sensitive. 5.5. The possible relationship between the enhancement of experimental anaphylaxis by H. pertussis vaccine and the questionable observation of the production of the clinical syndrome of paralysis following vaccination is discussed.

Anaphylactic shock in the pertussis vaccinated mouse.

Summary 1. It has been confirmed that H. pertussis vaccination of white mice produces a 50-fold increase in histamine sensitivity. 2. Mice immunized by an intraperitoneal injection of a mixture of H. pertussis vaccine and horse serum become markedly anaphylac-togenic to a challenging dose of the serum. Brucella abortus vaccine has a somewhat similar effect on anaphylaxis.

Histamine Sensitivity and Anaphylaxis in the Pertussis-Vaccinated Rat.

Summary Inoculation of the white rat with Hemophilus pertussis increases its sensitivity to histamine. Pertussis mixed with horse serum markedly enhances the ability of the antigen to produce anaphylactic sensitivity.

Anaphylactic Shock in the Pertussis Vaccinated Mouse. II. Diphtheria and Tetanus Toxoids as Antigens.

Summary 1. Mice immunized by an intraperitoneal injection of a mixture of H. pertussis and diphtheria toxoid become markedly anaphylactogenic to a challenging dose of toxoid. 2. Under the conditions of the experiments described pertussis vaccine has no enhancing effect on the anaphylactogenicity of tetanus toxoids.

The effect of nitrogen mustard on anaphylaxis in the pertussis-vaccinated mouse

Abstract The addition of 60 or 70 γ of nitrogen mustard (HN2) to a mixture of antigen and as little as 6 × 10 4 pertussis organisms enhanced the anaphylactic sensitization of mice. The optimum dose of vaccine without HN2 is 6 × 10 9 cells. When 50γ of HN2 was injected on the day before the antigen-pertussis mixture, there was no alteration of anaphylactic death rates. However, if given after the immunizing mixture, HN2 coused a decrease in the death rate of about 50 per cent. The administration of HN2 either before or after antigen alone did not produce a sensitization. A dose of 20 to 50γ of HN2 in combination with antigen and 6 × 10 8 or 10 9 Bordetella pertussis cells caused the elaboration of antibody-containing ascitic fluid; given one day prior to the pertussis, HN2 produced an increased accumulation. The inoculation of 6 × 10 9 pertussis organisms had no effect on toxicity when given before or after the HN2. A decrease in toxicity was noted when 50 to 70γ of HN2 was given together with 6 × 10 4 to 10 8 B. pertussis organisms.