Beverley Orchard

Response of subterranean clover, balansa clover and gland clover to lime when grown in mixtures on an acid soil

This study compared the relative tolerances of subterranean clover (Trifolium subterraneum L.), balansa clover (T. michelianum Savi.), and gland clover (T. glanduliferum Boiss.) to acid soil conditions. Seed yield, seedling density, herbage production, N2 fixation, and herbage mineral composition of the 3 legumes were assessed when grown on an acid soil (pHCa of 4.3 and 15% exchangeable Al [0–0.10 m]) with and without the addition of lime (CaCO3). Annual legume species were sown in a mixed sward together with burr medic (Medicago polymorpha L.), and in mixtures with either lucerne (Medicago sativa L.), chicory (Cichorium intybus L.), or phalaris (Phalaris aquatica L.). Due to drier than average seasonal conditions, none of the perennial species persisted beyond the first summer. Lime increased the herbage production of annual legumes by 18–22% and total pasture production by 14% in both 2002 and 2003. Subterranean clover was the most tolerant of the annual legumes to acid soil conditions, showing no visible toxicity symptoms and no response to lime in terms of seed yield. In contrast, both balansa and gland clovers exhibited visual symptoms of manganese toxicity in the absence of lime, with Mn concentrations in the shoots of 817 mg/kg and 626 mg/kg, respectively. Both species responded positively to lime with seed yields increasing by 45% and 124%, respectively. Lime increased the proportion of herbage N derived from N2 fixation by subterranean clover from 29 to 40% and by gland clover from 30 to 43%. Lime had no effect on the proportion of N2 fixed by balansa clover (29–31%), suggesting a suboptimal symbiosis of rhizobia with that species. Adding chicory or phalaris to the pasture mix increased sward herbage production in the establishment year by 39% and 21%, respectively. Based on leaf symptoms and herbage yield responses to lime, Mn toxicity was present in lucerne with tissue levels of up to 916 mg/kg, but no symptoms were observed in chicory (1129 mg/kg) or phalaris (403 mg/kg). Chicory and phalaris were more tolerant of acidity and high levels of Mn than lucerne, gland clover, and balansa clover. The study highlighted the value of the small-seeded annual legumes, balansa clover and gland clover, to the production of mixed pasture swards even in drier than average seasonal conditions. Although more sensitive to acid soils than subterranean clover, they set a greater number of seeds and, in the case of balansa clover, a greater weight of seed under moisture stress in the establishment year than the larger seeded subterranean clover.

Effect of companion perennial grasses and lucerne on seed yield and regeneration of subterranean clover in two wheatbelt environments

Seed production of subterranean clover (Trifolium subterraneum L.) in mixtures with lovegrass (Eragrostis curvula (Schrader) Nees cv. Consol), cocksfoot (Dactylis glomerata L. cv. Currie), phalaris (Phalaris aquatica L. cv. Sirolan), danthonia (Austrodanthonia richardsonii (Cashm.) H.P. Linder, cv. Taranna), and lucerne (Medicago sativa L. cv. Aquarius) was compared with pure and degraded (invaded by annual volunteers) annual subterranean clover pasture at 2 sites (Junee and Kamarah) in the southern wheatbelt of New South Wales. Seed yields, clover seedlings in winter, and the change in the proportion of 3 subterranean clover cultivars (Dalkeith, Seaton Park, Goulburn) when grown with and without perennials were assessed. The effect of thinning the perennials to 10 plants/m2 on clover seed set was examined at the drier site. Seed production of subterranean clover in the mixtures was depressed by up to 50% compared with the pure and degraded annual swards. Initial clover seed poduction in the mixtures was at least 60 kg/ha even in the drought year at the wetter site (Junee), and >85 kg/ha at Kamarah, the drier site (seedling establishment at Kamarah failed in the drought year). Clover seed reserves in the following 2 years progressively increased to >300 kg/ha in the perennial swards at Junee but were 650 kg/ha at Junee and >350 kg/ha at Kamarah. Reducing perennial density to 10 plants/m2 at the drier site increased clover seed yield about 3-fold in the first year compared with unthinned perennial swards. The increased seed yield was due to increased numbers of burrs set and increased seeds per burr and, in all perennial pasture treatments except lucerne, increased seed size. Clover seedling regeneration in 3rd and 4th year after sowing was substantially lower in the perennial-based mixtures than annual plots, with a significant (P < 0.05) positive correlation at both sites between clover seedling regeneration and seed bank size (1996, r2 = 0.46–0.64; 1997, r2 = 0.64–0.85). Following false breaks in early autumn, clover seedling populations were substantially higher in the pure and degraded clover treatments than in most perennial treatments. The proportion of the 3 cultivars present in the seed bank at the end of the pasture phase differed between sites but the sward type only influenced the proportion at the drier site. At the medium rainfall site, the later maturing cultivar Goulburn constituted 27–54% of the seed bank and the early flowering Dalkeith 25–46%, with unsown cultivars being insignificant ( <1%). At the low rainfall site, Dalkeith was the major component (33–52%) of the seed bank but the background population of unsown cultivars constituted 11–48%, the lowest proportion being in swards without a perennial component. The proportion of Goulburn was highest (23%) in the pure sward and lowest (10%) in lucerne and phalaris. It was concluded that subterranean clover could form relatively stable mixtures with perennials in medium rainfall environments, with clover populations increasing with time. In lower rainfall environments, clover seedling populations in perennial swards may be low due to reduced seed set and decreased seedling survival following early autumn rains. In these environments earlier maturing, hard-seeded cultivars are more likely to persist in mixtures and there is more potential for unsown cultivars to constitute a greater proportion of the sward. Decreasing perennial density offers scope for improving clover seed set and survival in these environments.

Lucerne, phalaris, and wallaby grass in short-term pasture phases in two eastern Australian wheatbelt environments. 1. Importance of initial perennial density on their persistence and recruitment, and on the presence of weeds

The influence of initial plant density on the changes in the populations of 3 perennial pasture species, lucerne (Medicago sativa L.), wallaby grass (Austrodanthonia richardsonii (Cashm.) H.P. Linder), and phalaris (Phalaris aquatica L.), over a 3-year pasture phase was examined in the wheatbelt of southern New South Wales. The perennials were sown at 5 rates in combination with subterranean clover (Trifolium subterraneum L.) at 2 locations, Kamarah [430 mm average annual rainfall (a.a.r.)] and Junee (550 mm a.a.r). The range in initial plant populations for lucerne, phalaris, and wallaby grass was 4–74, 8–94, and 2–20 plants/m2, respectively, at Kamarah and 11–120, 9–149, and 6–48 plants/m2 at Junee. When sown at higher densities, the density of lucerne and phalaris declined curvilinearly over the 3 years at both sites. At the 3 lower densities, phalaris populations remained constant at both sites. Lucerne, in contrast, declined over all densities at both sites except at the lowest density at the wetter site (Junee). The rate of decline in lucerne was negatively related (R2 = 0.75) to initial density at Junee, but not at Kamarah. The density of the native grass, wallaby grass, increased with time at both sites through seedling recruitment. The invasion of experimental plots by the summer weed Eragrostis cilianensis (All.) Vign. ex Janchen (stinkgrass) was restricted by lucerne and phalaris, with a negative curvilinear relationship between perennial density and E. cilianensis seedlings in both environments (R2 = 0.65–0.70). In contrast, wallaby grass was ineffective at suppressing E. cilianensis. By the third year, phalaris had significantly higher herbage yields in spring than lucerne and wallaby grass at both sites and phalaris yield was independent of density. Lucerne yields at this time increased with density only at the wetter site (R2 = 0.64), but wallaby grass yields responded to increasing density at both sites (R2 = 0.27–0.59). The experiment demonstrated that establishing higher initial perennial populations of lucerne and phalaris, which did not recruit during the experiment, will result in the maintenance of higher populations over the life of a 3–4 year pasture phase despite proportionally higher rates of plant loss. The size of the initial population was less critical for wallaby grass, which was able to increase in density through recruitment. High initial populations are likely to be an advantage for suppressing weeds in swards of perennial species with limited ability to increase their basal area, such as lucerne and wallaby grass, but will be less beneficial for species such as phalaris, which can compensate by greatly increasing its basal area at lower densities.

Impact of legume 'break' crops on the residual amount and distribution of soil mineral nitrogen

Important factors in the successful uptake of grain legumes by cereal growers have been their capacity to increase soil N and control cereal disease, as these have underpinned high yields in following wheat crops. However, alternative 1-year legume crops are required to introduce additional biodiversity and management flexibility for cereal growers. The effects on soil mineral N and potential contribution to soil total N of other legume enterprises were studied. These included vetch (Vicia bengalhensis) or clovers (mix of Trifolium alexandrinum, T. versiculosum, T. resupinatum) managed for green manure; pea (Pisum sativum), vetch, or clovers managed for silage; and clovers managed for hay. These were compared with pea and lupin (Lupinus angustifolius) managed for grain production. Wheat was also included as a control. The legumes were grown in acidic Red Kandasol soil at Wagga Wagga in southern New South Wales, in 1996, 1997, and 1998. Mineral N was measured in the autumn or winter of seasons 1997 and 1998 respectively. Amounts of stubble residue N were measured in all seasons. The green manure crops, particularly vetch, produced more mineral N than both grain legumes. The forage conservation crops (silage or hay) produced similar amounts of mineral N to grain pea and more than grain lupin. For the grain and green manure legume crops, variation in amounts of mineral N was explained by the total N content of legume stubble residue, but for the forage conservation crops, more mineral N was measured than was predictable from stubble N. The amounts of mineral N at different soil depths differed between legume treatments and experiments (sites and years). Based only on above-ground plant N, the green manure crops contributed more to increasing total soil N than grain legumes; in turn, the grain legumes contributed more than the forage conservation crops. It was concluded that alternative annual legume enterprises to grain legumes may provide at least similar enrichment of soil mineral N early in the following season, and that all annual legume enterprises may accumulate nitrate deep in the soil profile in some seasons. AR Leg ro l mi Jv et al

Lucerne, phalaris, and wallaby grass in short-term pasture phases in two eastern Australian wheatbelt environments. 2. Effect of perennial density and species on subterranean clover populations and the relative success of 3 clover cultivars of different maturity

The effect of the density of 3 perennial species, phalaris (Phalaris aquatica L.), wallaby grass (Austrodanthonia richardsonii Kunth), and lucerne (Medicago sativa L.), on seed set, regeneration, and the relative competitiveness of 3 cultivars of subterranean clover (Trifolium subterraneum L.) was examined in 2 environments in the south-eastern Australian wheatbelt. Seed yields of subterranean clover were inversely related to perennial density at both sites over the first 2 years, the relationship varying with perennial species. Phalaris depressed the seed yield of clover more than lucerne and wallaby grass in the second and third year at equivalent densities. Clover seed yield was positively related to clover herbage yield in late spring at both sites, and inversely related to perennial herbage yield. Clover seed yield displayed an increasing linear relationship with the proportion of light reaching the clover understorey in spring, which in turn was inversely related to perennial density and perennial herbage yield. Clover seedling regeneration in mixed swards in autumn was positively related to the size of the summer seed bank, but negatively related to perennial density. Clover seedling survival following a premature germination at Kamarah was inversely correlated to the density of phalaris and lucerne in the sward. The relative competitiveness of the 3 subterranean clover cultivars varied between sites, with climatic conditions (rainfall and growing-season length) having a greater effect on the relative cultivar performance than companion perennial species or density. The later maturing subterranean clover cv. Goulburn became the dominant cultivar at the wetter site, constituting 72% of the seed bank, but declined to only 3-8% of the seed bank at the drier site. The proportion of the early flowering cultivar Dalkeith in the seed bank increased over time at the drier site and was highest (53%) in plots with the highest perennial density. We concluded that although perennial pasture species will depress clover seed yield and subsequent regeneration, these effects could be minimised by reducing perennial densities and exploiting variations in competitiveness between perennial species as identified in this study. Sowing earlier maturing subterranean clover cultivars would only be an advantage in increasing clover content in low-rainfall environments. The findings suggest that clover seed reserves and regeneration could also be increased by using grazing management to reduce the level of shading of clover by perennials, a factor associated with reduced clover seed yield.

Oil concentration and composition of olives during fruit maturation in south-western New South Wales

This paper reports on an observational study of the oil and moisture content, and fatty acid profile, of olives (Olea europaea) for 15 tree groupings with groups consisting of trees with similar DNA patterns. Observations were taken at 2 sites (Wagga Wagga and Yanco) in south-western New South Wales, over 2 seasons. These sites represent the largest gene pool of developed olive trees in Australia. Differences in oil and moisture content were identified at both the Wagga Wagga and Yanco sites. At the former site, group 12 (˜cv. Tarascoa) had the highest oil content at full colour development in both 1998 and 1999 (50.1 and 45.5p dry weight, respectively) while at Yanco, group 15 (˜cv. Verdale) had the highest oil content in both years with 51.6 and 45.3%, respectively. The fatty acid profile was shown to be highly dependent upon tree group (i.e. cultivar), and with further study may be used as a method for discriminating among cultivars. The pattern of development of oil, moisture and fatty acids (palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acids) is also detailed for 4 trees in the Wagga Wagga grove over the ripening season in 1998 and 1999. For these trees, oil content (dry weight basis) increased rapidly in the first 15 weeks (January–April) while moisture declined except for a small increase at around 10 weeks (mid-March). Fatty acid development showed similar trends between the trees over the length of the study.

Influence of forage legume species, seeding rate and seed size on competitiveness with annual ryegrass (Lolium rigidum) seedlings

Annual legumes sown as short-term forage crops are an important non-chemical option for the control of herbicide-resistant annual ryegrass (Lolium rigidum L.). The relative ability of 5 annual forage legume species (Trifolium subterraneum L., T. michelianum Savi., T. alexandrinum L., Medicago murex Wild and Vicia benghalensis L.) to suppress annual ryegrass seedlings was examined in a glasshouse study. The experiment investigated the importance of legume species, seed size and seeding rate in influencing the relative competitiveness of the legumes. Balansa clover (T. michelianum) and berseem clover (T. alexandrinum), the smallest seeded of the legume species, were the most effective of the 5 legume species, when compared at equivalent seeding rates, at reducing ryegrass biomass. Legume leaf area and biomass were the 2 factors measured that were most closely associated with depressing ryegrass biomass and were themselves most influenced by legume species and seeding rate. Balansa clover and vetch (V. benghalensis) were the most effective at restricting the amount of light penetrating to 50% of the height of the canopy over a range of seeding rates. The study showed that the competitiveness of forage break crops can be optimised by maximising legume biomass through selecting small-seeded legume species with high relative growth rates and dense canopies and by increasing the seeding rate where seed costs are low.

Effect of gypsum on establishment, persistence and productivity of lucerne and annual pasture legumes on two grey Vertosols in southern New South Wales

Changes in pasture yield and botanical composition due to gypsum application were examined on Vertosols at two locations of differing soil sodicity, Grogan and Morangarell, in southern New South Wales. Two pasture treatments were examined. One was an annual pasture comprised of 3 annual legumes (2 subterranean clover Trifolium subterraneum L. cultivars, Clare and Riverina, and balansa clover T. michelianum Savi cv. Paradana), while the second treatment consisted of lucerne (Medicago sativa L.) cv. Aquarius sown in a mixture with the same annual legumes. Gypsum had no effect on the establishment or persistence of lucerne at either site. Gypsum increased the number of subterranean clover seedlings present in autumn in annual swards at the more sodic Grogan site in each of the 4 years, but provided no difference when the clover was in a mixture with lucerne. Annual legume seed yields in annual-only swards increased with gypsum by up to 58% at Grogan and 38% at Morangarell. Seed yields of both cultivars of subterranean clover declined as a proportion of the total annual legume seed bank when lucerne was included in the mixture, in contrast to balansa clover (at Grogan) and the naturalised annual legumes, burr medic (M. polymorpha L.) and woolly clover (T. tomentosum L.), which all increased in relative seed yield in the presence of lucerne. Total pasture production at the Grogan site increased with gypsum by up to 15% per annum in annual swards and 36% in lucerne swards depending on the season. Yield responses to gypsum by the lucerne component were observed in 10 of the 13 seasonal yield measurements taken at Grogan. However, total pasture yield and seasonal yields were unaffected by both gypsum and pasture type at the less sodic Morangarell site. It was concluded that sowing a diverse mixture of annual legumes or polycultures was conducive to maintaining productive pastures on these spatially variable soils. Lucerne dried the soil profile (0.15–1.15 m) more than annual pastures at both sites. The combination of gypsum and lucerne enhanced water extraction at depth (0.6–1.15 m) at the Grogan site increasing the size of the dry soil buffer whereas gypsum increased soil water at depth (>0.6 m) under annual swards.

Soil potassium relationships, uptake efficiency and availability for six distinctive soils in central and southern New South Wales, Australia

Most soils in eastern Australian contain abundant soil potassium (K) reserves, and it is often assumed that there are no problems with soil K status. However, soil K deficiency has been reported in selected locations, and for viticulture, there are potential problems with high soil K concentrations due to the application of winery wastewater. This study investigated different soil K variables and plant variables for six soils with distinctive properties from across central and southern New South Wales to determine the presence of soil K deficiency and to understand the effect of adding K on the dynamics of soil K availability. A glasshouse experiment compared the selected soils under three fertiliser K rates with forage kale as the test species. Highly significant differences (P < 0.001) were found for soil and fertiliser K rate effects for three measures of soil K (solution K, soln K; exchangeable K, exch. K; tetraphenyl borate K; TBK). Significant soil and fertiliser rate effects were detected (P < 0.001 and P = 0.04 respectively) for the plant shoot (stem and leaf) biomass and nutrient uptake efficiency (UPE) index, but no plant K deficiency was detected; in fact, luxury K consumption was likely. Quantification of K efficiency indices (UPE and utilisation efficiency, UTE) demonstrated significant differences between the soils in the ease with which K was removed. This was illustrated by the negative correlation between both UPE and UTE with final exch. K. From soil properties potentially related to soil K variables, a significant linear regression relationship (P = 0.05) was found for TBK with illite and clay content. By contrast, a linear regression relationship between exch. K and illite content only was weaker (P = 0.09). These relationships show how soil properties (especially mineralogy) can predict soil K variables. A significant positive log–log relationship was found between exch. K or TBK for 37 Queensland soils and the same soil K measures from this study, consistent with this relationship. This relationship indicates that TBK can be effectively predicted from measuring exch. K for a wide range of soils across eastern Australia, but more research is required to understand the value of TBK to predict soil K availability.

Impact of annual legume 'break' crops on the yield and quality of canola in comparison with the impact on yield of wheat

This study investigated the yield, oil and protein content of canola in response to alternative single-season legume crops, and compared the yield response with that of wheat. Two field trials were conducted in consecutive years at Wagga Wagga on the South West Slopes of New South Wales. The soil type was Red Kandasol. The legume treatments were field pea and vetch managed for silage production, vetch managed for green manure, a mixture of aerial seeding clovers (Berseem, Arrowleaf, Persian: 6 : 3 : 3) managed for silage, hay or green manure, and field pea and narrow-leaf lupin managed for grain. There was one wheat treatment managed for grain. In terms of growing season rainfall, the rainfall-use efficiency of canola was low and similar to that achieved on farms (5.1 kg/ha.mm rainfall). Yields of canola were less than potential water-limited yields and the canola equivalent wheat yields were less than comparative wheat yields. The constraint(s) on canola yield meant that nitrogen-limited yield potential was not reached, and in contrast to wheat in adjacent plots, there was no relationship between canola seed yield and soil mineral nitrogen available during seedling establishment of canola. However, relative to other legume treatments, antecedent crops of the clover mix depressed yield and total oil content, particularly in one year. It was concluded that under field conditions that constrained canola productivity to that similar to on-farm productivity, wheat may make more efficient use of legume nitrogen than canola. Were canola to be used as the response crop, antecedent legumes of vetch or pea, rather than aerial seeding clover, would give higher potential yield and total oil content of the canola.