Bhabatosh Banik
Indian Institute of Science
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Featured researches published by Bhabatosh Banik.
Inorganic Chemistry | 2016
Aditya Garai; Ila Pant; Samya Banerjee; Bhabatosh Banik; Paturu Kondaiah; Akhil R. Chakravarty
Ternary cobalt(III) complexes of curcumin (Hcur) and mitocurcumin [Hmitocur, a dicationic bis(triphenylphosphonium) derivative of curcumin] having a tetradentate phenolate-based ligand (H2L), namely, [Co(cur)(L)] (1) and [Co(mitocur)(L)]Cl2 (2), were prepared and structurally characterized, and their photoinduced cytotoxicity was studied. The diamagnetic cobalt(III) complexes show an irreversible Co(III)-Co(II) redox response and a quasireversible curcuminoid-based reduction near -1.45 and -1.74 V SCE, respectively, in DMF/0.1 M [(n)Bu4N](ClO4). The complexes exhibit a curcumin/mitocurcumin-based absorption band near 420 nm. Complex 1 was structurally characterized by X-ray crystallography. The structure contains the metal in a CoN2O4 distorted octahedral coordination arrangement with curcumin binding to the metal in its enolic form. Binding to cobalt(III) increases the hydrolytic stability of curcumin. Complex 2, having a dicationic curcuminoid, shows significant cellular uptake and photoinduced cytotoxicity compared to its curcumin analogue 1. The dicationic cobalt(III) complex 2 has significantly better cellular uptake and bioactivity than the neutral species 1. Complex 2 with mitochondrial localization releases the mitocurcumin dye upon exposure to visible light (400-700 nm) in human breast cancer MCF-7 cells through photoreduction of cobalt(III) to cobalt(II). Complex 2 displays a remarkable photodynamic therapy (PDT) effect, giving an IC50 value of ∼3.9 μM in visible light (400-700 nm) in MCF-7 cells while being much less toxic in the dark (>50 μM). The released mitocurcumin acts as a phototoxin, generating intracellular reactive oxygen species (ROSs). The overall process leads to light-controlled delivery of a curcuminoid (mitocur) into the tumor cells while the dye alone suffers from hydrolytic instability and poor bioavailability.
Carcinogenesis | 2016
Kumar Somyajit; Bhabatosh Banik; Sneha Saxena; Sharath Babu; Manoor Prakash Hande; Akhil R. Chakravarty; Ganesh Nagaraju
Although DNA interstrand crosslinking (ICL) agents such as mitomycin C, cisplatin and psoralen serve as potent anticancer drugs, these agents are known to have dose-limiting toxic effects on normal cells. Moreover, tumor resistance to these agents has been reported. Here, we show that trans-dichlorooxovanadium (IV) complex of pyrenyl terpyridine (VDC) is a novel photoinducible DNA crosslinking agent. By a combination of in vitro and ex vivo experiments including plasmid-based assays, we find that VDC forms monoadducts on the DNA and can be activated by UV-A and visible light to generate DNA interstrand crosslinks. VDC efficiently activates Fanconi anemia (FA) pathway of DNA interstrand crosslink repair. Strikingly, photoinduction of VDC induces prolonged activation of cell cycle checkpoint and a high degree of cell death in homologous recombination (HR)/ICL repair defective cells. Moreover, VDC specifically targets cells that express pathological RAD51C mutants. These data imply that VDC can be potentially used for cancer therapy and suggest that tumors arising in patients with gene mutations in FA and HR repair pathway can be specifically targeted by a photoactivatable VDC.
Journal of Inorganic Biochemistry | 2017
Arun Kumar; Ila Pant; Akanksha Dixit; Samya Banerjee; Bhabatosh Banik; Rupak Saha; Paturu Kondaiah; Akhil R. Chakravarty
Oxovanadium(IV) complexes [VO(L1/L2)Cl2]n+ (1,2) of (anthracenyl)terpyridine (An-tpy as L1 in 1, n=0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP+ as L2 in 2, n=1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. [VO(An-tpy-TPP)Br2]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP+ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed ~10 fold increase in cytotoxicity on exposure to visible light (400-700nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.
Organometallics | 2010
Basudev Maity; Mithun Roy; Bhabatosh Banik; Ritankar Majumdar; Rajan R. Dighe; Akhil R. Chakravarty
Dalton Transactions | 2013
Akhtar Hussain; Kumar Somyajit; Bhabatosh Banik; Samya Banerjee; Ganesh Nagaraju; Akhil R. Chakravarty
Dalton Transactions | 2014
Bhabatosh Banik; Kumar Somyajit; Ganesh Nagaraju; Akhil R. Chakravarty
European Journal of Inorganic Chemistry | 2012
Samya Banerjee; Akhtar Hussain; Puja Prasad; Imran Khan; Bhabatosh Banik; Paturu Kondaiah; Akhil R. Chakravarty
European Journal of Inorganic Chemistry | 2011
Bhabatosh Banik; Pijus K. Sasmal; Sovan Roy; Ritankar Majumdar; Rajan R. Dighe; Akhil R. Chakravarty
European Journal of Inorganic Chemistry | 2012
Babu Balaji; Bhabatosh Banik; Pijus K. Sasmal; Basudev Maity; Ritankar Majumdar; Rajan R. Dighe; Akhil R. Chakravarty
Inorganica Chimica Acta | 2013
Babu Balaji; Kumar Somyajit; Bhabatosh Banik; Ganesh Nagaraju; Akhil R. Chakravarty