Bingli Lei

Occurrence, sources and health risk assessment of polycyclic aromatic hydrocarbons in urban (Pudong) and suburban soils from Shanghai in China

A comprehensive investigation was conducted to the urban (Pudong) and suburban soils in Shanghai. A total of 154 soil samples were analyzed for 26 PAHs including highly carcinogenic dibenzopyrenes (DBPs). The total concentrations ranged from 25.8 to 7380 μg kg(-1) for Σ26PAHs and 18.8 to 6320 μg kg(-1) for 16 USEPA priority PAHs (Σ16PAHs), respectively. The BaP toxic equivalent (BaPeq) concentrations were between 6.41 and 2880 μg kg(-1) for Σ24PAHs, 1.11 and 620 μg kg(-1) for Σ16PAHs and 2.72 and 2250 μg kg(-1) for Σ4DBPs. The high PAH contamination in green land soils might originate mainly from local road traffic and industrial activities, and sewage sludge application or waste water irrigation for soil. Seven sources of soil PAHs in Shanghai were identified by positive matrix factorization (PMF) model. The mean risk quotient (m-RQ) values indicated that there were medium to high ecological risks in 9.10% of soil samples, pyrene (Pyr), benzo[b]fluoranthene (BbF) and benz[a]anthracene (BaA) were the major ecological risk drivers under agricultural use. The cancer risk (CR) values were within the acceptable range at 35.7%, 35.1% and 31.2% of sampling sites for children, youths and adults, respectively. The total lifetime carcinogenic risk (TLCR) values at 57.8% of sampling sites were within the acceptable range. Overall, cancer risks of soil PAHs in all sampling sites in the studied area were below the highest acceptable risk, suggesting that soil PAHs are unlikely to pose a significant cancer risk for population based on ingestion, dermal contact and inhalation exposure pathways.

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

The growth, development, and ERα and Vtg-I gene expressions of Japanese ricefish (Oryzias latipes; medaka) exposed to different concentrations of estriol (E3), including one environmentally relevant concentration, during embryo-adult life stages were evaluated. At the early life stage, fertilized eggs were exposed to 5, 50, 500, 5000ng/L E3 for 15days, and the hatched fry were exposed continuously to the same concentrations for an additional 15days. Exposure to 500 and 5000ng/L E3 resulted in adverse effects on hatchability and time to hatching. At 5000ng/L, the gross abnormality rate was increased and the number of females that hatched was twice that of males. When the fish were exposed to 5-5000ng/L E3 for further 60days, the male hepatosomatic index (HSI) was increased at 5000ng/L. The female gonadosomatic index (GSI) was decreased at 500 and 5000ng/L E3, while the male GSI at 5000ng/L E3 was increased and sex reversal was also found at this concentration. Quantitative RT-PCR showed that the hepatic vitellogenin-I (Vtg-I) genes were up-regulated in females at 500 and 5000ng/L E3 and in males at all E3 concentrations, whereas E3 did not affect estrogen receptor α (ERα) mRNA transcription. These results showed that E3 at environmental concentration of 5ng/L has no adverse effects on growth and development of the Japanese medaka. However, in this study, if we only focused on Vtg gene change in males, E3 had strong estrogenic effects on male medaka under the conditions of these experiments.

Effects of estrone on the early life stages and expression of vitellogenin and estrogen receptor genes of Japanese medaka (Oryzias latipes).

The fertilized eggs of Japanese medaka (Oryzias latipes) were exposed to estrone (E1) at 5-5000 ng L(-1) for 15 d, and the hatched fry were exposed continuously to the same concentrations for the additional 15 d. Adverse effects on hatchability, time to hatching, and gross abnormalities occurred at 50 ng L(-1) or above. Then the fry were divided into a continual exposure group, and a water recovery group. When the fry were exposed to E1 for another 60 d, there was a decrease in the hepatosomatic index (HSI) of males and the influence disappeared in the water recovery group. The gonadosonatic index (GSI) of females at 500 ng L(-1) decreased significantly in another 60 d exposure. While the fry were maintained in dechlorinated tap water for 60 d, a significant decrease in female GSI was observed at 50 ng L(-1) or above. An increased GSI was found in males in both continual exposure and water recovery groups at all E1 treatments. Quantitative RT-PCR showed that vitellogenin-I (Vtg-I) gene expressions in the female liver were significantly down-regulated at 50 ng L(-1) in the continual exposure group, and at 500 ng L(-1) in the water recovery group, while male Vtg-I genes were significantly up-regulated for all E1 treatments. In addition, all E1 treatments caused sex reversal of males. These results suggest that E1 at 5 ng L(-1) or above have unrecoverable impacts on the gonadal growth and development of medaka, even if only early life stages were exposed to E1.

In vitro profiling of toxicity and endocrine disrupting effects of bisphenol analogues by employing MCF-7 cells and two-hybrid yeast bioassay

The potentially adverse health implications of bisphenol A (BPA) have led to increasing use of alternative bisphenols (BPs). However, little is known about the toxicity of alternative BPs. In this study, the cytotoxicity, genotoxicity, intracellular ROS formation, and Ca2+ fluctuation effects of BPs on MCF‐7 cells were evaluated. At the same time, the estrogenic and thyroidal hormone effect potentials of six BPs were also evaluated using two‐hybrid yeast bioassay. The results showed that most BPs at 0.01–1 μM significantly increased cell viability in MCF‐7 cells and at higher exposure concentrations of 25–100 μM, they caused a significant decrease of cell viability. At the same time, these BPs also at 25–100 μM significantly increased LDH release of MCF‐7 cells. In addition, several BPs at 10–50 μM resulted in a significantly concentration‐depended increase in DNA‐damaging effect on MCF‐7 cells and elevated ROS production. Most BPs at 0.0001–10 μM significantly increased intracellular Ca2+ level. These results showed that bisphenol AF (BPAF) and thiodiphenol (TDP) exerted cell biological effect, estrogenic, and thyroidal effect potentials greater than those of BPA. The cytotoxicity and endocrine disrupting effects of other BPs are similar to or slightly lower than those of BPA. Therefore, as potential alternatives to BPA, endocrine disrupting effects and potential health harm of alternative BPs to human can also not be ignored.

Water quality criteria for 4-nonylphenol in protection of aquatic life

Here reproduction and death toxicity data were selected for 4-nonylphenol based on endocrine disrupting properties of the reproductive system. Assessment factor (AF) and species sensitivity distribution (SSD) curve were employed to derive criteria maximum concentration (CMC) and criteria continuous concentration (CCC) of three different 4-nonylphenols for protection of aquatic life. The results showed that the CMC and CCC based on SSD method and death toxicity data for three different 4-nonylphenols (CAS No: 104405, 25154523, 84852163) were 26.7, 13.6, 3.84 μg L−1 and 8.86, 2.21, 0.97 μg L−1, respectively. Based on SSD and reproductive toxicity data, the CCC values of different 4-nonylphenols (CAS No: 104405, 25154523) were 1.59 and 1.34 μg L−1, respectively. The CCC values obtained by the AF for three different 4-nonylphenol (CAS No: 104405, 25154523, 84852163) were 0.165, 1.03, 0.74 μg L−1 and 0.5, 0.5, 0.1 μg L−1, respectively, based on death toxicity data and reproductive toxicity data. The CCC values obtained by AF were all lower than the corresponding criteria values obtained by SSD, and the CCC values based on reproductive toxicity data were less than those based on the death toxicity data. This study provides a useful method for deriving water quality criteria for endocrine disruptors.

Atmospheric occurrence, homologue patterns and source apportionment of short- and medium-chain chlorinated paraffins in Shanghai, China: Biomonitoring with Masson pine (Pinus massoniana L.) needles.

A comprehensive survey was conducted to Masson pine (Pinus massoniana L.) needles widely distributed in Shanghai in order to investigate the levels and homologue group patterns of short- and medium-chain chlorinated paraffins (SCCPs and MCCPs), to identify and quantitatively assess source contributions to the total CPs in pine needle samples. The concentration ranged from not detected (ND) to 13,600ngg(-1) with a geometric mean (GM) value of 63.7ngg(-1) for ΣSCCPs, from 12.4 to 33,500ngg(-1) with a GM value of 677ngg(-1) for ΣMCCPs, and from 14.0 to 45,700ngg(-1) with a GM value of 768ngg(-1) for total CPs. For different sampling units, the pollution levels both for SCCPs and MCCPs in pine needles were in the same orders: Pudong>suburbs>Puxi>Chongming. These significant differences in SCCPs and MCCPs among four sampling units could be associated with difference in industrial activities and to some extent also in population density. All pine needle samples (n=131) were divided into 2 groups by hierarchical cluster analysis (HCA) for SCCPs and MCCPs, the most abundant homologue groups in the bulk of pine needle samples were C11Cl5-7 and C13Cl5-7 for SCCPs, and C14Cl7-8 and C15Cl7-8 for MCCPs. Correlation analysis suggested that SCCPs and MCCPs in pine needles in the studied area may be derived from different sources. Four sources for pine needles were identified by the FA-MLR model; their relative contributions to the total CP burden in pine needles were 18.0% for F1 (attributed to commercial SCCP mixture), 42.2% for F2 (attributed to commercial MCCP mixture), 29.3% for F3 (attributed to LRAT), and 10.5% for F4 (unknown source). CP contamination of atmospheric air by point sources and long-range atmospheric transport in Shanghai should receive more attention by local government.

The transepithelial transport mechanism of polybrominated diphenyl ethers in human intestine determined using a Caco-2 cell monolayer

&NA; Oral ingestion plays an important role in human exposure to polybrominated diphenyl ethers (PBDEs). The uptake of PBDEs primarily occurs in the small intestine. The aim of the present study is to investigate the transepithelial transport characteristics and mechanisms of PBDEs in the small intestine using a Caco‐2 cell monolayer model. The apparent permeability coefficients of PBDEs indicated that tri‐ to hepta‐BDEs were poorly absorbed compounds. A linear increase in transepithelial transport was observed with various concentrations of PBDEs, which suggested that passive diffusion dominated their transport at the concentration range tested. In addition, the pseudo‐first‐order kinetics equation can be applied to the transepithelial transport of PBDEs. The rate‐determining step in transepithelial transport of PBDEs was trans‐cell transport including the trans‐pore process. The significantly lower transepithelial transport rates at low temperature for bidirectional transepithelial transport suggested that an energy‐dependent transport mechanism was involved. The efflux transporters (P‐glycoprotein, multidrug resistance‐associated protein, and breast cancer resistance protein) and influx transporters (organic cation transporters) participated in the transepithelial transport of PBDEs. In addition, the transepithelial transport of PBDEs was pH sensitive; however, more information is required to understand the influence of pH. Graphical abstract Figure. No caption available. HighlightsTransepithelial transport mechanism of PBDEs in intestine tested using Caco‐2 cells.Passive diffusion dominated the transepithelial transport PBDEs in Caco‐2 cell.Transepithelial transport of PBDEs followed the pseudo‐first‐order kinetics process.Trans‐cell and trans‐pore process is the rate‐determining step in the PBDE transport.Transporters of P‐gp, MRP, BCRP, and OCTs participated in transepithelial transport.

Benzophenone-UV filters in personal care products and urine of schoolchildren from Shenzhen, China: Exposure assessment and possible source

The use of benzophenone (BP)-type UV filters in personal care products (PCPs) has rapidly increased in China over the past decade, leading to growing concerns on the potential adverse effects associated with the usage. Urine analysis is an ideal non-invasive approach for human biomonitoring of xenobiotics that are excreted mainly through urinary system. To investigate human exposure of PCPs to children from South China, we determined BP-type UV filters in a total of 156 commercial PCP goods covering 11 categories, as well as 280 urine samples collected from elementary school students in Shenzhen, China. Five BP analogues (i.e., BP1, BP2, BP3, BP8, and 4HB) were frequently detected in both PCPs and urine, among which BP3 was the dominant analogue, accounting for 96.3% of the total BPs in PCPs and 53.2% in urine, respectively. Sunscreens contained the highest BP concentrations (mean: 2.15 × 104 ng g-1) among all PCP goods. Girls exhibited higher urinary BP concentrations than boys, and body mass index positively influenced BP concentrations. However, no regional difference in urinary BP concentration was observed. The estimated dermal uptake of BPs from PCPs after considering the percutaneous absorption rates was much lower than the estimated dermal intake. The total daily excretion doses estimated from urinary BPs were 74.4 and 47.4 ng·kg-1bw day-1 for girls and boys, respectively. The higher usage of body lotions, hand lotions, and sunscreens by girls than boys (1.49 vs. 1.03 times week-1) might play an important role.

Low-concentration BPF induced cell biological responses by the ERα and GPER1-mediated signaling pathways in MCF-7 breast cancer cells

Bisphenol F (BPF), one of the alternatives to bisphenol A (BPA), can induce proliferation through the nuclear estrogen receptor ERα (estrogen receptor alpha) pathway in human breast cancer MCF-7 cells. However, the roles of membrane estrogen receptor GPER1 (G-protein-coupled receptor 1)-mediated signaling pathways in MCF-7 cell proliferation caused by BPF are unclear. The influence of BPF on MCF-7 cells was evaluated in terms of cell proliferation, intracellular calcium (Ca2+) fluctuations, and reactive oxygen species (ROS) generation. The molecular mechanisms of the cellular responses to low doses of BPF were studied through detecting the activations of ERα and GPER1-regulated PI3K/PKB or AKT (phosphatidylinotidol 3-kinase/protein kinase B) and ERK1/2 (extracellular-signa1-regulated kinase 1/2) signals. At 0.01-1 μM, BPF significantly promoted cell proliferation and elevated the levels of intracellular ROS and Ca2+. At these concentrations, BPF also significantly upregulated protein expressions of ERα, GPER1, c-myc, and cyclin D and phosphorylations of PKB and ERK1/2. Specific signal inhibitors decreased PKB and ERK1/2 phosphorylations and attenuated the effects of BPF. Silencing of GPER1 also significantly decreased BPF-induced cell proliferation. These results indicate that activating the GPER1-PI3K/PKB and ERK1/2 signals by low doses of BPF can regulate the response of MCF-7 cells and that ERα also influences the effects of exposure to BPF on the cells. The present study suggests a new mechanism by which BPF exerts relevant estrogenic action in cancer cells and also highlights the potential risks in using BPF as an alternative to BPA.