Björn Holmquist

Pitfalls in the AMS radiocarbon-dating of terrestrial macrofossils

The AMS 14 C technique has the advantage that small samples of Late Quaternary age can be dated with high accuracy, and that errors due to reservoir effects can be avoided if specifically determined terrestrial micro- and macrofossils are measured. However, to obtain such high-accuracy measurements, it is important how small samples are handled prior to treatment in the radiocarbon laboratories. Here we present a set of 51 AMS 14 C measurements, of which 31 dates gave expected ages and 20 dates resulted in anomalously young ages, despite the fact that all samples consisted of clearly identified Late Weichselian terrestrial plant macrofossils. To evaluate possible sources of error, we compared these samples in respect to preparation methods, sample storage and sample weight. Our results show that the long-term storage of wet macrofossil samples appears to have a significant effect on the radiocarbon age obtained, even when the samples are kept cool. Fungi or micro-organisms may easily be incorporated into a sample during preparation and identification, and can easily contribute to the contamination of a sample, if stored cool and wet for several months.

Pre-degenerated nerve grafts enhance regeneration by shortening the initial delay period.

In the present study we tested how nerve grafts with different pre-degeneration periods (1-28 days) influenced the early regenerative response in the rat sciatic nerve. The sciatic nerve on the right side was crushed and after 1-28 days of pre-degeneration, a 10 mm segment was used as an autologous nerve graft and transposed to a freshly made 10 mm long nerve defect on the left side. The regeneration distance was measured by the sensory pinch test 2-10 days after nerve repair. A newly developed mathematical model was used to calculate regeneration rates and initial delay periods from the measured regeneration distances. Pre-degenerated nerve grafts improved nerve regeneration by decreasing the initial delay period as compared to fresh nerve grafts without affecting the regeneration rate. Only one day of pre-degeneration was sufficient to reduce the initial delay period from 3.6 days to 1.7 days. The maximal effect on the initial delay period was achieved after 3 days of pre-degeneration. The initial delay period at later pre-degeneration intervals (7-14 days) was about 1 day. The effect persisted for at least 28 days of pre-degeneration. The regeneration rate was 1.5 mm/day for fresh nerve grafts and between 1.8-2.1 mm/day for pre-degenerated grafts. The results suggest that the effects of pre-degeneration are not only due to the increased cell proliferation in the graft, but that also trophic and/or inflammatory mechanisms may be of importance. Grafts pre-degenerated by crush may have clinical implications since they are easy to perform if an elective nerve grafting procedure is planned.

Predegeneration enhances regeneration into acellular nerve grafts

In the present study, we determined the regeneration rate and the initial delay in rat sciatic nerve grafts first made hypercellular by predegeneration then acellular by freeze-thawing. 7-day predegenerated nerve pieces from the distal nerve stump on the right side were made acellular by repeated freeze-thawing and inserted as grafts into a 10-mm long freshly created defect on the left contralateral side. Freshly made (no predegeneration period) acellular nerve grafts were used as controls. Both types of grafts supported outgrowth of regenerating axons as demonstrated by the sensory pinch test. However, the predegenerated acellular nerve grafts had a significantly shorter initial delay period (2.7 days) as compared with freshly made acellular nerve grafts (9.5 days). The initial delay period for predegenerated acellular nerve grafts was similar to that for fresh cellular nerve grafts but significantly longer than that for predegenerated cellular nerve grafts [24]. The rate of regeneration appeared independent of the type of grafts used. We suggest that modifications of the basal lamina and/or factors produced during the predegeneration period by non-neuronal cells survive the freeze-thawing cycle and account for the decrease in the initial delay period.

Analysis of film coating thickness and surface area of pharmaceutical pellets using fluorescence microscopy and image analysis

A method is presented which enables geometrical characterisation of pharmaceutical pellets and their film coating. It provides a high level of details on the single pellet level. Image analysis was used to determine the coating thickness (h) applied on the pellets and the surface area (A) of the pellet cores. Different definitions of A and h are evaluated. Hierarchical analysis of variance was used to resolve different sources contributing to the total variance. The variance within pellets and the variance between pellets were found as significant sources of variation. Special emphasis was put on evaluation of A/h due to its influence on the release rate of an active drug substance from the pellet core. The pellet images were thus used to predict variations in the release rate using a mathematical model as a link between the image data and the release rate. General aspects of image analysis are discussed. The method would be useful in calibration of near infrared spectra to h in process analytical chemistry.

Effects of organic solvents on motor activity in mice

Groups of male mice were exposed via inhalation to methylene chloride, perchloroethylene, toluene, trichloroethylene or 1,1,1-trichloroethane. The exposures were started at 2300 h. Generation of vapor was stopped after 1 h. Motor activity of the animals during the exposures was measured with a Doppler radar. Several concentrations of each solvent were tested. Concentrations could be found for all solvents at which they initially increased the motor activity. When the generation of vapor was terminated and the concentration started to decline, a new phase of changes in motor activity was induced. At this phase, motor activity was in most cases influence in the opposite direction to that at the beginning of the exposure. Trichloroethylene concentrations could be found which gave no increase in activity at the start of exposure but a prominent decrease at termination. The lowest concentration at which effects could be seen was different for the different solvents. Perchloroethylene was more and 1,1,1-trichloroethane less potent than the other solvents in inducing motor activity. The time pattern of the motor activity alterations was specific for each solvent. Both the concentration and the rate of the concentration increase were responsible for the effects on motor activity. The differences between the solvents probably reflect differences in their site of action, their distribution and their biotransformation.

Trichloroethylene: Effects on body and organ weights in mice, rats and gerbils

The influence of continuous inhalation of 150 ppm trichloroethylene (TCE) on body, liver, spleen, and kidney weights in rats, mice, and mongolian gerbils was tested. An age dependent decrease in body weight gain was observed in female rats exposed to TCE. All 3 species showed liver enlargement caused by the exposure. The effect was much more pronounced in mice, in which the increase was 60--80%, than in rats and gerbils where it was only 20--30%. After the end of the TCE-exposure the liver weights of the mice decreased rapidly. After 5 days of rehabilitation the weight was only 10--20% higher than that of the controls. This difference persisted for at least 25 days. The spleen weight appeared unaffected or somewhat smaller in TCE-exposed animals of all species. An increased kidney weight (15%) was observe din TCE-exposed gerbils. This effect was less pronounced in mice and rats. Effects on the liver have earlier been seen only after exposure to concentrations much higher than that used in the present study. This difference in results is proposed to be due to the different schedules used for the exposure.

Moments and cumulants of the multivariate normal distribution.

Expressions in vector notation are given for the central moments, the non–central moments and the cumulants of arbitrary order of the multivariate normal distribution

The relationship between annual varve thickness and maximum annual discharge (1909-1971)

Annually laminated (varved) sediments from the River Angermanalven, mid-central Sweden, have been used to construct an annual 2000-year long record of varve thickness. Maximum daily annual discharge and mean varve thickness for the years 1909-1971 are significantly correlated (r = 0.87). A relationship between maximum daily annual discharge for the observed period (1909-1971) and varve thickness was determined. The return time of two exceptionally thick varves in the 2000-year long record at the years 658 and 492 AD were estimated and their likelihood estimated based on a Gumbel frequency analysis

The d-variate vector Hermite polynomial of order k

Abstract The multivariate Hermite polynomials occur in Edgeworth and saddle-point approximations to the distributions of random vector variables. Explicit expressions in vector notation for the multivariate Hermite polynomials of arbitrary order are given and shown to be a special case of a general addition property of Hermite polynomials. General properties of these polynomials are given in vector notation, including an integral representation, the orthogonality property, differential properties, and recurrence relations as well as inversion formulas. Various expansions of a multivariate normal density are given in terms of a multivariate normal density with different mean, different covariance, or both. In special cases these results give Mehlers formula.

The climatic significance of clastic varves in the Ångermanälven Estuary, northern Sweden, AD 1860 to 1950

Multiple regression analyses were applied to decipher the correlation between monthly discharge, precipitation and temperature records and annual varve-thickness data from river Ångermanälven north-central Sweden between 1860 and 1950. Over the whole 90-year period we found generally a strong correlation between spring/summer precipitation and annual varve thickness. However, there are clear indications that precipitation during the months of January and March–May was more important for the formation of varves in the early part of the twentieth century, while January, March, April and June seem to have been more significant during the later part of the nineteenth century. Monthly temperatures and annual varve thickness, on the other hand, did not show any significant correlations over the whole 90-year period, but, when split into 30-year periods, a dependence of varve thickness on October (1862–90) and March (1919–50) temperatures could be observed. Our results show that it is important to test each specific varve record against instrumental data sets before any conclusions can be drawn in terms of precipitation and/or temperature dependence. They also emphasize the necessity to correlate varve-thickness records against long instrumental series in order to detect any changing forcing mechanisms.