Bjørn T. Barlaup

Potential disease interaction reinforced: double-virus-infected escaped farmed Atlantic salmon, Salmo salar L., recaptured in a nearby river

The role of escaped farmed salmon in spreading infectious agents from aquaculture to wild salmonid populations is largely unknown. This is a case study of potential disease interaction between escaped farmed and wild fish populations. In summer 2012, significant numbers of farmed Atlantic salmon were captured in the Hardangerfjord and in a local river. Genetic analyses of 59 of the escaped salmon and samples collected from six local salmon farms pointed out the most likely source farm, but two other farms had an overlapping genetic profile. The escapees were also analysed for three viruses that are prevalent in fish farming in Norway. Almost all the escaped salmon were infected with salmon alphavirus (SAV) and piscine reovirus (PRV). To use the infection profile to assist genetic methods in identifying the likely farm of origin, samples from the farms were also tested for these viruses. However, in the current case, all the three farms had an infection profile that was similar to that of the escapees. We have shown that double-virus-infected escaped salmon ascend a river close to the likely source farms, reinforcing the potential for spread of viruses to wild salmonids.

Salmon lice increase the age of returning Atlantic salmon

The global increase in the production of domestic farmed fish in open net pens has created concerns about the resilience of wild populations owing to shifts in host–parasite systems in coastal ecosystems. However, little is known about the effects of increased parasite abundance on life-history traits in wild fish populations. Here, we report the results of two separate studies in which 379 779 hatchery-reared Atlantic salmon smolts were treated (or not) against salmon lice, marked and released. Adults were later recaptured, and we specifically tested whether the age distribution of the returning spawners was affected by the treatment. The estimates of parasite-induced mortality were 31.9% and 0.6% in the River Vosso and River Dale stock experiments, respectively. Age of returning salmon was on average higher in treated versus untreated fish. The percentages of fish returning after one winter at sea were 37.5% and 29.9% for the treated and untreated groups, respectively. We conclude that salmon lice increase the age of returning salmon, either by affecting their age at maturity or by disproportionately increasing mortality in fish that mature early.

Gene flow from domesticated escapes alters the life history of wild Atlantic salmon

Interbreeding between domesticated and wild animals occurs in several species. This gene flow has long been anticipated to induce genetic changes in life-history traits of wild populations, thereby influencing population dynamics and viability. Here, we show that individuals with high levels of introgression (domesticated ancestry) have altered age and size at maturation in 62 wild Atlantic salmon Salmo salar populations, including seven ancestral populations to breeding lines of the domesticated salmon. This study documents widespread changes to life-history traits in wild animal populations following gene flow from selectively bred, domesticated conspecifics. The continued high abundance of escaped, domesticated Atlantic salmon thus threatens wild Atlantic salmon populations by inducing genetic changes in fitness-related traits. Our results represent key evidence and a timely warning concerning the potential ecological impacts of the globally increasing use of domesticated animals.

Reference point based management of Norwegian Atlantic salmon populations.

SUMMARY While management according to biological reference points is well established for many commercial marine fisheries, similar systems for more leisure based fisheries for freshwater fishes are less common. This paper describes the scientific foundation for management according to conservation limits and management targets for Norwegian populations of Atlantic salmon, a highly valued and heavily exploited anadromous fish species. Based on stock recruitment relationships during the freshwater phase, the biomass of females necessary to attain the carrying capacity (yielding average maximum recruitment) has been established as conservation limits for each of the 439 Norwegian populations. Using a simulation model based on reported catch and estimates of exploitation rates, the probability and percentage attainment of the conservation limits have been assessed annually since 2008, and exploitation advice provided for 176 of the largest populations. The number of populations that attained their conservation limits increased substantially after the new management scheme was introduced, despite that the number of returning salmon remained at historical low levels. Overall the populations evaluated in 2011 were at 95% of their conservation limits compared to 91% in 2008 and 85% in 2005. The improvement could largely be attributed to reduced exploitation rates, due to new restrictions in both the marine and river fisheries. The new management scheme also improved the catch statistics and stimulated data acquisition for management. Implementation of management according to conservation limits has been a success in terms of attaining the main management goal of protectingtheAtlanticsalmonpopulationsbyensuring that an increasing number of the populations likely are at their maximum reproductive capacity. Long-term increasesinfisheriesyield,thesecondarymanagement ∗ Correspondence: Dr Torbjorn Forseth e-mail: torbjorn.forseth@ nina.no goal, are likely to be attained, but remain to be documented. Reference point based management of Atlantic salmon exemplifies management within the intersectionoffisheriesmanagementandconservation biology, borrowing principles from both sides.

Can the river location within a fjord explain the density of Atlantic salmon and sea trout

Abstract The rivers that drain into the Hardangerfjord were historically known to have numerous populations of both sea trout (Salmo trutta) and Atlantic salmon (Salmo salar). After a decline in catches during the last decades many of the rivers have been closed for fishing. In this study we use snorkelling observations from rivers and catch statistics from 2004 to 2011 to describe the current situation and analyse the patterns of density of wild salmon, sea trout and escaped farmed salmon in the Hardangerfjord rivers. We hypothesize that some of the variance in density of salmon and sea trout can be explained by the location of the river in the fjord, with fish from rivers with a longer fjord exposure having a lower density. A median number of 3.5 salmon×ha−1 and 14.9 sea trout×ha−1 were observed in the rivers. Farmed salmon were observed in all rivers and constituted on average 23.3% of the total number of observed salmon. For salmon, there was an inverse log-linear relationship between density in the river and migration distance to coast, with a higher density in rivers with a shorter migration distance. For sea trout there was no evident relationship with location within the fjord. We suggest that the spatial patterns observed for salmon and sea trout can be related to the species-specific differences in habitat use within the fjord system.

A conservation plan for Atlantic salmon (Salmo salar) and anadromous brown trout (Salmo trutta) in a region with intensive industrial use of aquatic habitats, the Hardangerfjord, western Norway

Abstract Extensive use of aquatic habitats, mainly for hydropower and aquaculture, has a negative impact on anadromous salmonid populations of the Hardangerfjord region, western Norway. High infection levels of salmon lice, and high proportions of escaped farmed salmon in spawning rivers, appear to violate the goals in the ‘Strategy for an Environmentally Sustainable Aquaculture Industry’ set by the Norwegian government. An overview of the anadromous populations in the fjord, their status and the major threats are presented. A conservation plan with mitigation efforts consisting of seven steps is presented: (1) genetic assessment of Atlantic salmon and anadromous brown trout populations, (2) reducing gene flow from escapees, (3) reducing infection pressure from salmon lice, (4) conduct an assessment of the freshwater habitats for anadromous salmonids and then implement it in order to restore smolt production, (5) efforts to reduce risk of river pollution from agriculture and industry and minimize impacts from hydropower production, (6) when and where necessary and practical, plant out eyed eggs from the Norwegian Genebank to increase parr and smolt production, and finally, (7) monitor spawning populations and parr densities to evaluate potential effects of the mitigation efforts. Experience and knowledge gained through the plan will be useful for other regions with similar challenges. We call for an initiative to establish a national fund under democratic and public control, where funding can be obtained for projects which focus on mitigation efforts and conservation of salmonid populations.

Improved growth in stunted brown trout (Salmo trutta L.) after reliming of Lake Hovvatn, Southern Norway

The chronically acidic Lake Store Hovvatn and the adjoining pond Pollen in southernmost Norway were limed in March 1981. The two locations were stocked with brown trout (Salmo trutta L.) at low and high densities in Hovvatn and Pollen, respectively. After 6 yr of reacidification, the locations were relimed in July 1987. Growth depression during the reacidification process in spite of low fish densities and superabundance of food was observed in Lake Store Hovvatn. Three months after reliming, a substantial growth response was found in trout from Lake Store Hovvatn; Mean annual length increment was 68% higher than that of the preceding year. In Pollen, reliming had no apparent effect on growth. In both populations reliming caused increased swimming activity measured as an increase in CPUE-values. These results show that the growth response to liming depends on population density and food availability. Moreover, the results indicate that the food conversion rate of the trout is negatively affected in acid waters.

Effects of hydropeaking on the spawning behaviour of Atlantic salmon Salmo salar and brown trout Salmo trutta

An in situ camera set-up was used to study the spawning activity of Atlantic salmon Salmo salar and brown trout Salmo trutta throughout two consecutive seasons in a spawning area affected by hydropower-related pulse flows due to hydropeaking. The purpose was to test whether the flow variation discouraged spawning in shallow areas or motivated spawning into areas with elevated risk of incubation mortality. There were more S. salar observed on the spawning ground during days with high discharge. The presence of S. salar in the spawning grounds was not affected by the hydropeaking cycles of the preceding night. Female S. salar were observed preparing nests within the first hour after water discharge had increased to levels suitable for spawning. In contrast, the number of S. trutta was not correlated with flow and nest preparation was also observed at a discharge corresponding to the lowest discharge levels during a hydropeaking cycle. Survival was generally high in nests excavated the following winter, with only 5·4% suffering mortality due to dewatering. The results suggest that S. salar may respond rapidly to variable-flow conditions and utilize short windows with suitable flows for spawning. Smaller S. trutta may utilize low-flow conditions to spawn in areas that are not habitable by larger S. salar during low flow.

Microsatellite DNA used for parentage identification of partly digested Atlantic salmon (Salmo salar) juveniles through non-destructive diet sampling in salmonids

Abstract Predation during early life history is an important component of fitness in salmonids. Farmed Atlantic salmon display lower survival in the wild in comparison to wild salmon; however, the underlying mechanisms remain unknown. Salmon eggs from 69 families of farmed, hybrid and wild parentage were planted into a river. Following swim-up, 760 brown trout predators were non-lethally sampled. Of the trout, 4.2% had ingested salmon fry (0–15 fry/trout). From a total of 48 salmon fry recovered from trout stomachs, 46 were successfully identified to family using microsatellites. Of the 69 planted families, 29 were represented among the predated salmon fry; however, there were no significant differences in susceptibility to predation between the three groups (farm, wild and crosses), but the power of resolution was low due to small sample sizes. Nevertheless, we have successfully demonstrated that microsatellites can be used to address natural selection via diet analysis of predators in a natural system.

Incomplete mixing of limed water and acidic runoff restricts recruitment of lake spawning brown trout in Hovvatn, southern Norway

Repeated liming of Hovvatn during the 1981–1995 period assured successful reintroduction of lake spawning brown trout, Salmo trutta. Poor natural recruitment to the population was associated with low survival during early life stages (before hatching) as shown by the 0.5, 3.5, 0.9 and 1.0% of live embryos found in natural redds during the 1992–1995 period, respectively. The low survival was most likely caused by the combination of shallow spawning areas (<2.0 m) and acidic runoff (pH 4.0–4.8) which overlayed the limed part of the water body during the ice covered period. It is therefore concluded that this type of episodic acidification poses a major threat to lake spawning salmonids, and that it can retard or inhibit biotic recovery towards preacidified conditions expected as a result of liming. Addition of limestone gravel (8–32 mm) onto spawning grounds was an efficient alternative liming strategy as 33–36% live embryos were found in this substrate. Conversely, the trout actively avoided additions of shellsand, a behaviour most likely caused by the small particle size of shellsand (3–7 mm) relative to natural spawning gravel.