Bogomir Prokic

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.

Renal vasography in mink (Mustela vison)

Abstract This paper presents the results of the research on renal blood vessels of the mink. The main goal of this research is to provide a unique insight in renal vessel constellation and thereby contributes to the amount and quality of the available data on the cardiovascular system of the mink, since the data found in the available literature regarding this specific field is rather poorly documented. Blood vessels of the mink kidney were studied by dissection and corrosion injection technique. The renal artery and its branches, and the lobar, interlobar and interlobular arteries are terminal arteries and they do not anastomose between each other. The arterial system is characterized by two independent vascular areas, supplied by the dorsal and ventral lobar artery. Lobar arteries divide the kidney horizontally into a dorsal and ventral half, while lobar veins divide it vertically into the cranial and caudal half. The renal vein is formed from the cranial and caudal lobar vein. The obtained results are compared with relevant literature findings on renal blood supply of a dog and other sympatric carnivores.

Histomorphometric evaluation of bone regeneration using autogenous bone and beta-tricalcium phosphate in diabetic rabbits

Background/Aim The mechanism of impaired bone healing in diabetes mellitus includes different tissue and cellular level activities due to micro- and macrovascular changes. As a chronic metabolic disease with vascular complications, diabetes affects a process of bone regeneration as well. The therapeutic approach in bone regeneration is based on the use of osteoinductive autogenous grafts as well as osteoconductive synthetic material, like a β-tricalcium phosphate. The aim of the study was to determine the quality and quantity of new bone formation after the use of autogenous bone and β-tricalcium phosphate in the model of calvarial critical-sized defect in rabbits with induced diabetes mellitus type I. Methods The study included eight 4-month-old Chincilla rabbits with alloxan-induced diabetes mellitus type I. In all animals, there were surgically created two calvarial bilateral defects (diameter 12 mm), which were grafted with autogenous bone and β-tricalcium phosphate (n = 4) or served as unfilled controls (n = 4). After 4 weeks of healing, animals were sacrificed and calvarial bone blocks were taken for histologic and histomorphometric analysis. Beside descriptive histologic evaluation, the percentage of new bone formation, connective tissue and residual graft were calculated. All parameters were statistically evaluated by Friedman Test and post hock Wilcoxon Singed Ranks Test with a significance of p < 0.05. Results Histology revealed active new bone formation peripherally with centrally located connective tissue, newly formed woven bone and well incorporated residual grafts in all treated defects. Control samples showed no bone bridging of defects. There was a significantly more new bone in autogeonous graft (53%) compared with β-tricalcium phosphate (30%), (p < 0.030) and control (7%), (p < 0.000) groups. A significant difference was also recorded between β-tricalcium phosphate and control groups (p < 0.008). Conclusion In the present study on the rabbit grafting model with induced diabetes mellitus type I, the effective bone regeneration of critical bone defects was obtained using autogenous bone graft. [Projekat Ministarstva nauke Republike Srbije, br. 175021].

A. Hepatica in European ground squirrel (Citellus Citellus) compared to other experimental animals

Also, at the section Literature following reference should be stated in full: Blagojević Miloš, Vitorović Duško, Adamović Ivana, Nešić Ivana, Brkić Zlata, Zdravković Marija, Đorđević Milena, Jović Slavoljub, Zorić Zoran, Ćupić Dejana, Vascularization of the kidney of the ground squirrel (Citellus citellus) in comparison with other experimental animals, Veterinarski glasnik, 2015 69(1-2):31-40 https://doi.org/10.2298/ VETGL1502031B

Corrective surgery of external sexual organs in female pseudo hermaphrodite dog

Hermaphroditism is a congenital anomaly characterized by the presentation of sexual characteristics of both sexes in one individual. Hermaphrodites can occur in every mammal species. Real and pseudo hermaphrodites can be distinguished. Both ovaries and testes (ovotestes) are presented in real hermaphrodites, while the conformation of the individual does not correspond to any sex in particular. Pseudo hermaphrodites are more frequent. Male pseudo hermaphrodites have testes and female external genital characteristics, while female pseudo hermaphrodites have ovaries and rudimentary external genitals reassembling to masculine ones. Corrective surgical removal of a rudimental penis in a female pseudo hermaphrodite dog is presented in the paper.