Boris Paskhover

Quorum Sensing: Fact, Fiction, and Everything in Between

Publisher Summary Quorum sensing was commonly referred to as autoinduction. Autoinduction was originally described for the Gram-negative marine organism Vibrio fischeri in early 1970s. The series of experiments conducted by Kempner and Hanson (1968) revealed induction of bioluminescence in freshly inoculated V. fischeri. There is no doubt that the practical applications of quorum-sensing research carry enormous potential; however, it is crucial to realize that the direction of scientific exploration has always been influenced by the opinions of the scientific community. There are numerous examples in the history of science of appealing yet inaccurate functional theories that directed research onto a path of ambiguity. The attempt to utilize the relatively simple AI-2 detection assay initiated numerous questions, which led to uncover conflicting theories in every major aspect of quorum-sensing research. The very function of quorum sensing is not completely clear. The evolution of this process in bacteria could possibly have been driven by the need to sense the flow dynamics of their immediate environment, as opposed to the need for a concerted response. Since multiple factors could influence the direction of development in evolution, both theories could be correct and therefore have a right for coexistence. Conversely, the theory of AI-2-mediated interspecies communication seems likely to remain weak or even fall apart from insufficient supportive results.

Patterns of Change in Facial Skeletal Aging

Importance Research in facial aging has focused on soft-tissue changes rather than bony changes despite evidence of the importance of underlying bony structural changes. Research has also been limited by comparing different patients in separate age groups rather than the same patients over time. Objective To longitudinally document patterns of change in the facial skeleton and determine a consistent methodology for measuring these changes. Design, Setting, and Participants Case series study of university hospital system records using facial computed tomographic (CT) images timed at least 8 years apart in adults initially aged 40 to 55 years with no history of facial surgery who required repeated facial imaging that included the entire midface and cranium. Main Outcomes and Measures Face CTs were analyzed for 3-dimensional constructions and 2-dimensional measurements to document changes in glabellar, piriform, and maxillary angles and piriform height and width. Results Fourteen patients (5 men, 9 women; mean [SD] age, 51.1 [5.8] years) with mean (SD) follow-up of 9.7 (1.4) years were eligible for 2-dimensional analysis, which revealed statistically significant decreases in mean (SD) glabellar angles (from 68.8° [7.6°] to 66.5° [8.6°]) and maxillary angles on both the right (from 82.5° [6.3°] to 81.0° [7.1°]) and left (from 83.0° [5.8°] to 81.0° [7.0°]), as well as increases in mean (SD) piriform width (from 24.5 [1.6] mm to 25.5 [1.3] mm). Nine patients (3 men, 6 women; mean [SD] age, 51.4 [6.3] years) with mean (SD) follow-up imaging at 9.6 (1.5) years were eligible for 3-dimensional analysis, which revealed statistically significant decreases in mean (SD) maxillary angles (from 56.5° [6.6°] to 51.6° [7.6°]) and piriform angles (from 50.8° [3.4°] to 49.1° [3.4°]). Statistically significant differences between the sexes were also noted: Initial mean (SD) glabellar angle for men was 61.7° (5.7°) vs 72.7° (5.4°) for women, with final values of 57.9° (4.9°) vs 71.2° (6.0°). Mean (SD) maxillary angle initial values were 87.8° (6.1°) (right) and 87.1° (4.9°) (left) for men, with 79.6° (4.3°) and 80.6° (5.0°) for women, respectively. Final values were 87.0° (4.4°) and 86.9° (4.1°) for men and 77.7° (6.1°) and 77.7° (6.2°) for women, respectively. Mean (SD) piriform height for men was 35.0 (2.0) mm initially and 35.5 (2.1) mm finally, vs 31.3 (2.8) and 31.6 (3.0) mm for women, respectively. Conclusions and Relevance Our pilot study of repeated CT images of patients over several years supports previous studies of bony facial aging and further characterizes these changes. This study is the first, to our knowledge, to document bony changes of the face in the same group of patients at different time points to better characterize facial aging. We also detail an improved methodology to study bony aging to contribute to additional research in the field. Level of Evidence NA.

Investigating Complications Associated With Occipital Nerve Stimulation: A MAUDE Study: COMPLICATIONS IN ONS

The objectives of this study are to utilize the MAUDE data base to enhance our understanding of the complication profile for Occipital Nerve Stimulation, a therapy for which the current level of evidence is limited. Additionally, it is our objective to describe a systematic approach to processing the MAUDE data, which addresses its flaws and enhances its utility.

Nasal Distortion in Short-Distance Photographs: The Selfie Effect

Nasal Distortion in Short-Distance Photographs: The Selfie Effect The selfie, or self-photograph, has rapidly become one of the major photographic modalities of our time; in 2014 alone, there were over 93 billion selfies taken on Android phones per day.1,2 Despite the ease with which selfies are taken, the short distance from the camera causes a distortion of the face owing to projection, most notably an increase in nasal dimensions. According to a poll by the American Academy of Facial Plastic and Reconstructive Surgeons, 42% of surgeons reported patients seeking cosmetic procedures for improved selfies and pictures on social media platforms.3 We present a mathematical model to describe the distortive effects, prove the increased perceived nasal size in selfies, and calculate the magnitude of this effect from different camera distances. No patient intervention or contact was made during this study and was therefore exempt from institutional review board review.

Endoscopic Pericranial Flap Repair of Occipital-Frontal Electrode Erosion

INTRODUCTION Occipital-frontal nerve stimulation is an off-label therapy for treating chronic refractory migraine and orofacial pain. Though effective, patients experience a high rate of complications including lead migration and erosion through the overlying skin. CASE DESCRIPTION We present a case of frontal electrode erosion that was revised via pericranial flap repair. The patient presented with multiple lead migrations, necessitating multiple revision surgeries with eventual frontal wound dehiscence. The choice was made to wrap the electrode in a pericranial flap to prevent recurrent lead migration. Two weeks postoperatively, the wound was well healed and the patient reported that the midline electrode was functioning properly. DISCUSSION Pericranial flap revision confers little additional risk when compared with simple wound closure, and the surgeon can proceed without total electrode removal, additional incisions, or lead tunneling. The flap provides a highly vascular additional layer of stability to the electrode, reducing the likelihood of further lead exposure without compromising the efficacy of the device. These results suggest that endoscopic pericranial flap revision is a viable technique for the repair of occipital nerve stimulation lead erosions.

Minimally Invasive Trigeminal Ablation: Transoral Approach for Targeting V2

BACKGROUND Trigeminal neuralgia (TN) is a chronic orofacial pain syndrome, which manifests as severe pain in the distribution of any trigeminal nerve branch. Though traditionally responsive to anticonvulsant therapy, TN can become refractory to medications and require surgical intervention. CASE DESCRIPTION We present a case of V2 TN that was treated with minimally invasive trigeminal ablation. The patient presented with a 6-year history of type 1 TN and had failed the maximum tolerated doses of carbamazepine and gabapentin. There was no evidence of vascular compression on neuroimaging. After the patient refused stereotactic radiosurgery, she was offered minimally invasive trigeminal ablation. At 5 months postoperatively the patient reported complete alleviation of pain with tolerable sensorineural numbness. CONCLUSIONS The endoscopic approach allows for precise targeting of V2, which is ideal in patients undergoing targeted neuroablation for pain. This is the first documented case of a transoral endoscopic approach toward ablative V2 TN management.

Minimally Invasive Trigeminal Ablation: Novel Transoral Technique for Targeted Treatment of V3

BACKGROUND Trigeminal neuralgia (TN) is a chronic orofacial pain syndrome that presents with debilitating shooting pains in the V3 nerve distribution. The condition is traditionally responsive to anticonvulsant therapy; however, cases that become refractory to medications often require surgical intervention. CASE DESCRIPTION We present a case of TN that was treated with minimally invasive trigeminal ablation. The patient presented with a 6-year history of TN that had been resistant to medical management. The patient opted for minimally invasive management, refused stereotactic radiosurgery, and was treated using the minimally invasive trigeminal ablation approach. At 16 weeks postoperatively, the patient reported complete alleviation of pain with minimal sensorineural numbness. CONCLUSIONS The endoscopic approach allows for precise targeting through visual guidance, which is ideal in patients undergoing neuroablation for pain within specific nerve distributions. This is the first documented case of an endoscopic minimally invasive transoral approach toward ablative TN management.

Adverse Events Associated With Aesthetic Dermal Fillers: A 10-Year Retrospective Study of FDA Data:

This study investigates the reported adverse events arising with various aesthetic dermal fillers by type and brand name. It reveals rare but dangerous complications associated with a widely used elective procedure. The authors queried and compiled the MAUDE database for injury cases reported with aesthetic dermal fillers between January 2007 and July 2017. A total of 5024 individual cases were then queried for adverse events using specific keywords and grouped into categories for analysis. Categories and keywords were determined after querying a large portion of the samples for common adverse events. The most common complications associated with aesthetic dermal filler use were nodule formation (2952 cases), infection (2575 cases), inflammation (711 cases), allergic complications (594 cases), and vascular complications (590 cases). The most common fillers associated with an adverse event were Juvederm Voluma XC (1050 cases), Sculptra (879 cases), and Radiesse (620 cases). These fillers represent 3 filler types: hyaluronic acid fillers, poly-l-lactic acid fillers, and calcium hydroxylapatite fillers, respectively. Injectable dermal fillers are frequently used cosmetic products around the world. To safely and effectively use these products, health care providers and patients must be fully aware of the proper techniques and risks associated with each product. Many of the complications reported were known to be possible, but others were previously only reported as individual case studies. The rates of occurrence for both well-known and lesser known complications may be higher than originally thought.

Pediatric facial injuries: Hitting close to home

PURPOSE The aims of this study were as follows: To determine the national incidence of facial fractures among infants, toddlers, and children; to evaluate the types of facial fractures; and to analyze common products/activities associated with the fractures. MATERIALS AND METHODS A retrospective review was conducted to explore patterns of facial fractures among infants (<1 year), toddlers (1-3 years), and children (3-5 years), using the National Electronic Injury Surveillance System (NEISS) from the Consumer Product Safety Commission (CPSC). The database was searched for emergency department (ED) visits involving facial fractures sustained by children 1 month through 5 years of age from 2004 through 2016 and analyzed for patient demographics, primary diagnosis and associated products/activities. Subset analyses were performed between age groups to determine the relationship between causes of injury and age using extrapolated national incidences. RESULTS A total of 1507 ED visits for facial fractures among infants, toddlers, and children were obtained and extrapolated to an estimated total of 39,388 ED visits, averaging 3030 annually. The majority of these ED visits were for facial fractures that occurred at home. Most facial fractures sustained at the age period of 1 month-5 years old were nasal fractures. Facial fractures in infants were caused most commonly by floors or flooring materials, likely due to falls, while household items/furniture-related injuries were the most common cause of facial fractures in both toddlers and children. Facial fractures from playground equipment and riding toys were more common in children than in the other age groups. CONCLUSION The reported trends in the incidence by age group and types of fracture can assist physicians by serving as supplement for clinical history and examination when encountering this challenging patient population. Our findings highlight preventative opportunities, particularly in baby care items and beds for reducing facial fractures in infants. Similarly, preventative opportunities are highlighted in household items and furniture for lowering the incidence of facial fractures in toddlers and children. Our study also suggests ensuring safe handling of playground equipment and riding toys for the prevention of facial fractures in children.

Assessment of YouTube as an Informative Resource on Facial Plastic Surgery Procedures

Assessment of YouTube as an Informative Resource on Facial Plastic Surgery Procedures YouTube is a major modality for patient education. During the last decade, videos documenting facial plastic surgery procedures, patient experiences, and medical commentary have garnered hundreds of millions of views. The growing prevalence of YouTube as a primary source of medical information has prompted investigators to evaluate video quality and creator qualification.1,2 To date, the quality of facial plastic surgery content on YouTube has not been evaluated.