Boris Polak

Purification and characterization of porphyromonas gingivalis outer membrane antigens

Porphyromonas gingivalis is strongly associated with periodontal disease. Significant titres of specific IgG antibodies to P. gingivalis can be found in healthy individuals and those with periodontitis. In this study, 22 outer membrane antigens ranging from 15.5 to 107.6 kDa were recognized by sera from persons with periodontitis and controls. Serum from individuals with periodontitis showed a significantly higher IgG response to a 31.4-kDa antigen (p < 0.05); serum from those with gingivitis demonstrated a significantly higher response to a 15.5-kDa antigen (p < 0.05). The response to the 15.5-kDa antigen might represent a protective immune response while that to the 31.4-kDa could serve as a marker for disease susceptibility. These two antigens were purified to homogeneity and their N-terminal amino acid sequences determined. The sequences did not correspond to any previously described P. gingivalis antigens. The role of these two antigens in the pathogenesis of periodontal disease remains to be determined.

Effect of CD4/CD8 depletion on IFNg levels in P. gingivalis-infected mice

Corticosteroids are widely used today in the medical treatment of many chronic illnesss. It has hem well docunnented that corticosteroids affect hone metabolism and this rnaie concemas as so the effect of coeticosteroid treatent on orthodontic tooth movemiernt. The aimn of this study was to determine Ithe effect of an elevated physiologic level of corticosteroid on bone rmnodeling daring orthodontic movement. Twelve 9-weak old adult male Wisuar rate were divided into two groups- a corticosteroid treated group (n=6) and a control group (n=6). The corticosteroid treate group was admiinistered ltngik of oral prednisolone daily for a 12-day induction period, while the control group received the equivalent volumes of saline for the tame liTme period. Following thin induction period, an orthodontiC appiance was ligated between the maxillary first left molar and two nmaxillary central incisors such thata menia force of 30g wasW T R W generated. The molar on the right skide was used an the son-appliance control. All sanimals were sacrificedIT WDR after 12 days of appliance wear. The magnitude of tooth mnovement was recoorded. Maxillse were fixed, demineralised and processed to paraffin. Sagittal sections of the first molar were staned with harmatoxylin and cosin and for turtrate-resistant acid phosphatase (TRAP) activity. For thin dose of prednisolone, there are no significant differences (p<0.05) in the magnitude of tooth imovement between the treated and control groups. Steroid treaed rats displayed lens root resorption along the length of the root on the coespressive side as well as fewer TRAP positive cells widthin the PDL space of the comspressive side, conmpaed to the non-sterid control (p.Z0.05). There was more TRAP activity recorded along the tension-side alveolar bone surface (cervcal third) in the steroid treated rats comspared to the son steroid controls (pr0O05). The rate of orthodontic movements was unaffected by lessgIsa of orednisolone. The easth of root renemtion alon th menial comprenson side and the TRAP activity at the comoression side PDL were both reduced. suagenlise that a susorenion of clastic activity had taken place. This study was supported by an ADRF grat.

16S rRNA gene based PCR detection of Bacteroides forsythus in pooled subgingival plaque

Corticosteroids are widely used today in the medical treatment of many chronic illnesss. It has hem well docunnented that corticosteroids affect hone metabolism and this rnaie concemas as so the effect of coeticosteroid treatent on orthodontic tooth movemiernt. The aimn of this study was to determine Ithe effect of an elevated physiologic level of corticosteroid on bone rmnodeling daring orthodontic movement. Twelve 9-weak old adult male Wisuar rate were divided into two groups- a corticosteroid treated group (n=6) and a control group (n=6). The corticosteroid treate group was admiinistered ltngik of oral prednisolone daily for a 12-day induction period, while the control group received the equivalent volumes of saline for the tame liTme period. Following thin induction period, an orthodontiC appiance was ligated between the maxillary first left molar and two nmaxillary central incisors such thata menia force of 30g wasW T R W generated. The molar on the right skide was used an the son-appliance control. All sanimals were sacrificedIT WDR after 12 days of appliance wear. The magnitude of tooth mnovement was recoorded. Maxillse were fixed, demineralised and processed to paraffin. Sagittal sections of the first molar were staned with harmatoxylin and cosin and for turtrate-resistant acid phosphatase (TRAP) activity. For thin dose of prednisolone, there are no significant differences (p<0.05) in the magnitude of tooth imovement between the treated and control groups. Steroid treaed rats displayed lens root resorption along the length of the root on the coespressive side as well as fewer TRAP positive cells widthin the PDL space of the comspressive side, conmpaed to the non-sterid control (p.Z0.05). There was more TRAP activity recorded along the tension-side alveolar bone surface (cervcal third) in the steroid treated rats comspared to the son steroid controls (pr0O05). The rate of orthodontic movements was unaffected by lessgIsa of orednisolone. The easth of root renemtion alon th menial comprenson side and the TRAP activity at the comoression side PDL were both reduced. suagenlise that a susorenion of clastic activity had taken place. This study was supported by an ADRF grat.