Bozena Witek

Effect of ethanol administration on activities of some lysosomal hydrolases in the mouse

1. The studies were carried on 98 random 6-week-old male mice. The mice were divided into control and experimental groups. The experimental animals were given 10% and 20% ethanol solution daily for 7, 14 and 28 days. 2. In the lysosomal fractions of the liver, kidneys, muscle and brain, the activities of beta-galactosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, alanyl-aminopeptidase, leucyl-aminopeptidase, lysosomal esterase and lysosomal lipase were affected. 3. The changes in enzyme activities in the investigated tissue were related to the time and concentration of the administered ethanol.

The effect of small doses of mercury on the level of selected lysosomal enzymes in the plasma and lymphocytes of sheep

1. The 12-month-old ewes, Slovak Merinos breed, were given 40 mg of mercury daily for 28 days in the form of HgCl2. 2. Administration of mercury had a significant influence on the activity of the investigated lysosomal enzymes in the plasma and lymphocytes of sheep.

The influence of glucagon on the concentration of triacylglycerols and cholesterol in the liver and kidney of mice

Abstract. The experiment was performed on sexually mature male Swiss mice to determine the effect of pharmacological doses of glucagon administered over a period of 4 or 8 days on the concentration of triacylglycerols and cholesterol in liver and kidneys of mice selected for fast rate of body weight gain and unselected ones, kept on a high-protein and low-protein diet. On the basis of the results obtained it was concluded that the selection and the diet, as well as glucagon injections, were lowering the content of triacylglycerols and cholesterol in the examined organs of mice, in many cases confirmed in a statistically significant way.