Brian L. Clevinger

Monoclonal antibody with high affinity for 1,25-dihydroxycholecalciferol.

We have developed a monoclonal antibody capable of detecting 1 pg/ml of 1,25-dihydroxycholecalciferol. At a dilution of 1:80,000 of ascitic fluid this antibody has an apparent KD of 3.3 X 10(-11) ML-1. The immunogen used was a vitamin D analogue, calcitroic acid [1 alpha, 3 beta-dihydroxy-9, 10 seco-24-nor 5, 7, 10 (19) cholatriene-23-oic acid], conjugated to bovine serum albumin. Although this antibody is extremely sensitive, it also recognizes other important vitamin D3 metabolites.

Antibody Diversity Patterns and Structure of Idiotypic Determinants on Murine Anti- α(1 → 3) Dextran Antibodies

Idiotypic (variable-region) antigenic determinants have been extremely important to the study of immunoglobulins. These antigenic determinants have been widely used to examine variable-region expression in immunoglobulin populations because they offer a simple, sensitive, serological approach to variable-region structure. There are many different categories of idiotypic determinants: ligand-modifiable (binding site determinants), ligand-nonmodifiable (framework determinants), VL-specific, VH-specific, and those requiring both VL and VH. In addition, determinants shared by variable regions known to be structurally different are called IdX, cross-reactive, or public idiotypes, while those apparently restricted to identical variable regions are IdI, individual, or private idiotypes.

JH1 peptide induces antibodies to a common immunoglobulin determinant as detected by cell-binding analysis

In order to more accurately determine the distribution of antigenic determinants detected by antisera to hypervariable-region and JH1 peptides, we measured the frequency of lymphocytes stained with these sera by flow cytometry. None of the sera specific for HV1, HV2 or HV3 peptides stained significant numbers of lymphocytes, but those specific for JH1 reacted with nearly all B-cells.