Bruce Y. Yang

Quantitative assessment of graded burn wounds in a porcine model using spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI).

Accurate and timely assessment of burn wound severity is a critical component of wound management and has implications related to course of treatment. While most superficial burns and full thickness burns are easily diagnosed through visual inspection, burns that fall between these extremes are challenging to classify based on clinical appearance. Because of this, appropriate burn management may be delayed, increasing the risk of scarring and infection. Here we present an investigation that employs spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) as non-invasive technologies to characterize in-vivo burn severity. We used SFDI and LSI to investigate controlled burn wounds of graded severity in a Yorkshire pig model. Burn wounds were imaged starting at one hour after the initial injury and daily at approximately 24, 48 and 72 hours post burn. Biopsies were taken on each day in order to correlate the imaging data to the extent of burn damage as indicated via histological analysis. Changes in reduced scattering coefficient and blood flow could be used to categorize burn severity as soon as one hour after the burn injury. The results of this study suggest that SFDI and LSI information have the potential to provide useful metrics for quantifying the extent and severity of burn injuries.

An Overview of Three Promising Mechanical, Optical, and Biochemical Engineering Approaches to Improve Selective Photothermolysis of Refractory Port Wine Stains

During the last three decades, several laser systems, ancillary technologies, and treatment modalities have been developed for the treatment of port wine stains (PWSs). However, approximately half of the PWS patient population responds suboptimally to laser treatment. Consequently, novel treatment modalities and therapeutic techniques/strategies are required to improve PWS treatment efficacy. This overview therefore focuses on three distinct experimental approaches for the optimization of PWS laser treatment. The approaches are addressed from the perspective of mechanical engineering (the use of local hypobaric pressure to induce vasodilation in the laser-irradiated dermal microcirculation), optical engineering (laser-speckle imaging of post-treatment flow in laser-treated PWS skin), and biochemical engineering (light- and heat-activatable liposomal drug delivery systems to enhance the extent of post-irradiation vascular occlusion).

The Effect of Glenohumeral Position on the Shoulder After Traumatic Anterior Dislocation

Background Previous cadaveric studies suggest that positioning the shoulder in an externally rotated position reduces displaced Bankart lesions through a coaptation effect. Hypothesis We hypothesized that positioning the glenohumeral joint in an externally rotated position creates contact pressure between the subscapularis and the anterior labrum. Study Design Descriptive laboratory study Methods Eight cadaveric shoulders were used. Contact pressure between the subscapularis and labrum was measured in varying glenohumeral positions using a Tekscan pressure monitor. The position of the anterior band of the inferior glenohumeral ligament was also digitized in those positions. All shoulders were tested in the intact condition, following a surgically created Bankart lesion and following anterior shoulder dislocation. These conditions were also verified by measuring glenohumeral translation and joint forces. Results For all 8 specimens, the contact pressure between the subscapularis and the anterior labrum was negligible with the humerus externally rotated up to 90° at all abduction angles in intact, surgically created Bankart, and dislocated specimens. There were several glenohumeral positions where the anterior band of the inferior glenohumeral ligament strain in the intact specimens was similar to the postdislocation condition, that is, no statistically significant difference between intact and dislocated specimens. These positions included 30° of glenohumeral abduction with external rotation between 0° and 60°, as well as 45° of abduction with external rotation of 0° and 60°. The anterior band of the inferior glenohumeral ligament strain and glenohumeral anterior-posterior translation measurements revealed that the surgically created Bankart lesion does not simulate the conditions after anterior dislocation of the shoulder. Conclusion External rotation of the shoulder does not create contact pressure between the subscapularis and the anterior labrum before or after anterior dislocation. Clinical Relevance The efficacy of external rotation immobilization after anterior-inferior shoulder dislocation is not likely to be related to coaptation of the Bankart lesion by the subscapularis.

A novel cadaveric model for anterior-inferior shoulder dislocation using forcible apprehension positioning.

A novel cadaveric model for anterior-inferior shoulder dislocation using forcible apprehension positioning is presented. This model simulates an in vivo mechanism and yields capsulolabral lesions. The scapulae of 14 cadaveric entire upper limbs (82 +/- 9 years, mean +/- standard deviation) were each rigidly fixed to a custom shoulder-testing device. A pneumatic system was used with pulleys and cables to simulate the rotator cuff and the deltoid muscles (anterior and middle portions). The glenohumeral joint was then positioned in the apprehension position of abduction, external rotation, and horizontal abduction. A 6-degree-of-freedom load cell (Assurance Technologies, Garner, North Carolina) measured the joint reaction force that was then resolved into three orthogonal components of compression force, anteriorly directed force, and superiorly directed force. With the use of a thrust bearing, the humerus was moved along a rail with a servomotor-controlled system at 50 mm/s that resulted in horizontal abduction. Force that developed passively in the pectoralis major muscle was recorded with an independent uniaxial load cell. Each of the glenohumeral joints dislocated anterior-inferior, six with avulsion of the capsulolabrum from the anterior-inferior glenoid bone and eight with capsulolabral stretching. Pectoralis major muscle force as well as the joint reaction force increased with horizontal abduction until dislocation. At dislocation, the magnitude of the pectoralis major muscle force, 609.6 N +/- 65.2 N was similar to the compression force, 569.6 N +/- 37.8 N. A cadaveric model yielded an anterior dislocation with a mechanism of forcible apprehension positioning when the appropriate shoulder muscles were simulated and a passive pectoralis major muscle was included. Capsulolabral lesions resulted, similar to those observed in vivo.

Utility of spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) to non-invasively diagnose burn depth in a porcine model.

Surgical intervention of second degree burns is often delayed because of the difficulty in visual diagnosis, which increases the risk of scarring and infection. Non-invasive metrics have shown promise in accurately assessing burn depth. Here, we examine the use of spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) for predicting burn depth. Contact burn wounds of increasing severity were created on the dorsum of a Yorkshire pig, and wounds were imaged with SFDI/LSI starting immediately after-burn and then daily for the next 4 days. In addition, on each day the burn wounds were biopsied for histological analysis of burn depth, defined by collagen coagulation, apoptosis, and adnexal/vascular necrosis. Histological results show that collagen coagulation progressed from day 0 to day 1, and then stabilized. Results of burn wound imaging using non-invasive techniques were able to produce metrics that correlate to different predictors of burn depth. Collagen coagulation and apoptosis correlated with SFDI scattering coefficient parameter [Formula: see text] and adnexal/vascular necrosis on the day of burn correlated with blood flow determined by LSI. Therefore, incorporation of SFDI scattering coefficient and blood flow determined by LSI may provide an algorithm for accurate assessment of the severity of burn wounds in real time.

Glenohumeral joint translation after arthroscopic thermal capsuloplasty of the posterior capsule

Treatment of recurrent posterior or multidirectional glenohumeral instability in athletes with traditional operative management has produced variable results at long-term follow-up. The purpose of this study was to determine whether an arthroscopic thermal capsuloplasty of the posterior capsule with a radiofrequency probe significantly decreases anterior-posterior glenohumeral translation. Successive posterior and anterior loads of 10, 15, and 20 N were applied sequentially to 7 cadaveric shoulder joints that were mounted in a translation testing apparatus with an electromagnetic tracking device measuring posterior and anterior glenohumeral translation. Arthroscopic thermal capsuloplasty was then performed on the posterior capsular tissue with a radiofrequency probe. The identical posterior-anterior loading protocol was then repeated, and translations were recorded. The results demonstrated no statistically significant differences in the mean posterior translation measurements before and after arthroscopic thermal capsuloplasty of the posterior capsule for the 10-N (+8.9%), 15-N (-3.1%), or 20-N (-1.8%) load (P >.50 to.62). Slightly greater changes occurred in anterior translation after posterior capsuloplasty at 10 N (-1.0%), 15 N (-6.0%), and 20 N (-10.3%). However, these changes were not found to be significant either (P =.06 to.62). The results of this study demonstrated that neither posterior nor anterior glenohumeral translation was significantly decreased by thermal capsuloplasty of the posterior capsule. Perhaps the lack of substantial collagenous material in the thin posterior capsule accounts for the inability of thermal capsuloplasty to be effective in this region.

In vitro quantitative assessment of total and bipolar shoulder arthroplasties: A biomechanical study using human cadaver shoulders

BACKGROUND Glenohumeral arthroplasty has produced results far inferior to those seen with hip and knee joint procedures. Therefore, the objective of this study was to evaluate the biomechanical parameters of the glenohumeral joint before and after total shoulder arthroplasty and bipolar hemiarthroplasty in 12 different positions simulating overhead activities. METHODS Six matched pairs of cadaveric shoulders were used with a custom shoulder testing system to quantify the joint reactive force, contact areas, contact patterns and contact pressures in 12 different positions simulating overhead activities. The entire study was performed for the anatomic glenohumeral joint and following total shoulder arthroplasty on one side, and for the anatomic glenohumeral joint and following bipolar shoulder hemiarthroplasty on the contra-lateral side. FINDINGS There was a significant increase in posterior force following total shoulder arthroplasty in two positions of horizontal adduction (P<0.05). In positions of increased glenohumeral abduction and horizontal adduction, the contact pressures and patterns were greatly altered following both total shoulder arthroplasty and bipolar hemiarthroplasty procedures compared to the anatomic state. INTERPRETATION The alterations in joint reaction force, contact pressure and contact patterns following shoulder arthroplasty suggest a possible etiology for glenoid component edge loading in patients following total shoulder arthroplasty. This edge loading may lead to a rocking phenomenon of the glenoid component and subsequent loosening.

Intraoperative, real-time monitoring of blood flow dynamics associated with laser surgery of port wine stain birthmarks.

Port‐wine stain (PWS) birthmarks affect ∼22 million people worldwide. After several treatment sessions, complete disappearance of the PWS occurs in only ∼10% of treated patients. There is a need to develop a new strategy to improve the efficacy of each treatment session and the overall treatment outcome. The study objective was to determine how intraoperative measurements of blood flow correlate with treatment response assessed several weeks post treatment.

Fiber-based laser speckle imaging for the detection of pulsatile flow

In endodontics, a major diagnostic challenge is the accurate assessment of pulp status. In this study, we designed and characterized a fiber‐based laser speckle imaging system to study pulsatile blood flow in the tooth.

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

Here, we review our current knowledge on the etiology and treatment of port-wine stain (PWS) birthmarks. Current treatment options have significant limitations in terms of efficacy. With the combination of 1) a suitable preclinical microvascular model, 2) laser speckle imaging (LSI) to evaluate blood-flow dynamics, and 3) a longitudinal experimental design, rapid preclinical assessment of new phototherapies can be translated from the lab to the clinic. The combination of photodynamic therapy (PDT) and pulsed-dye laser (PDL) irradiation achieves a synergistic effect that reduces the required radiant exposures of the individual phototherapies to achieve persistent vascular shutdown. PDL combined with antiangiogenic agents is a promising strategy to achieve persistent vascular shutdown by preventing reformation and reperfusion of photocoagulated blood vessels. Integration of LSI into the clinical workflow may lead to surgical image guidance that maximizes acute photocoagulation, which is expected to improve PWS therapeutic outcome. Continued integration of noninvasive optical imaging technologies and biochemical analysis collectively are expected to lead to more robust treatment strategies.