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Astrobiology | 2011

The O/OREOS Mission: First Science Data from the Space Environment Survivability of Living Organisms (SESLO) Payload

Wayne L. Nicholson; Antonio J. Ricco; Elwood Agasid; Christopher Beasley; Millan Diaz-Aguado; Pascale Ehrenfreund; Charles Friedericks; Shakib Ghassemieh; Michael Henschke; John W. Hines; Christopher Kitts; Ed Luzzi; Diana Ly; Nghia Mai; Rocco L. Mancinelli; Michael McIntyre; Giovanni Minelli; Michael Neumann; Macarena Parra; Matthew Piccini; R. Mike Rasay; Robert Ricks; Orlando Santos; Aaron Schooley; David Squires; Linda Timucin; Bruce Yost; Anthony Young

We report the first telemetered spaceflight science results from the orbiting Space Environment Survivability of Living Organisms (SESLO) experiment, executed by one of the two 10 cm cube-format payloads aboard the 5.5 kg Organism/Organic Exposure to Orbital Stresses (O/OREOS) free-flying nanosatellite. The O/OREOS spacecraft was launched successfully to a 72° inclination, 650 km Earth orbit on 19 November 2010. This satellite provides access to the radiation environment of space in relatively weak regions of Earths protective magnetosphere as it passes close to the north and south magnetic poles; the total dose rate is about 15 times that in the orbit of the International Space Station. The SESLO experiment measures the long-term survival, germination, and growth responses, including metabolic activity, of Bacillus subtilis spores exposed to the microgravity, ionizing radiation, and heavy-ion bombardment of its high-inclination orbit. Six microwells containing wild-type (168) and six more containing radiation-sensitive mutant (WN1087) strains of dried B. subtilis spores were rehydrated with nutrient medium after 14 days in space to allow the spores to germinate and grow. Similarly, the same distribution of organisms in a different set of microwells was rehydrated with nutrient medium after 97 days in space. The nutrient medium included the redox dye Alamar blue, which changes color in response to cellular metabolic activity. Three-color transmitted intensity measurements of all microwells were telemetered to Earth within days of each of the 48 h growth experiments. We report here on the evaluation and interpretation of these spaceflight data in comparison to delayed-synchronous laboratory ground control experiments.


Astrobiology | 2012

The O/OREOS Mission: First Science Data from the Space Environment Viability of Organics (SEVO) Payload

Andrew Mattioda; Amanda Cook; Pascale Ehrenfreund; Richard C. Quinn; Antonio J. Ricco; David Squires; Nathan Earl Bramall; Kathryn L. Bryson; Julie Diane Chittenden; Giovanni Minelli; Elwood Agasid; Lou Allamandola; Chris Beasley; Roland Burton; Greg Defouw; Millan Diaz-Aguado; Mark Fonda; Charles Friedericks; Christopher Kitts; David Landis; Mike McIntyre; Michael Neumann; Mike Rasay; Robert Ricks; Farid Salama; Orlando Santos; Aaron Schooley; Bruce Yost; Anthony Young

We report the first science results from the Space Environment Viability of Organics (SEVO) payload aboard the Organism/Organic Exposure to Orbital Stresses (O/OREOS) free-flying nanosatellite, which completed its nominal spaceflight mission in May 2011 but continues to acquire data biweekly. The SEVO payload integrates a compact UV-visible-NIR spectrometer, utilizing the Sun as its light source, with a 24-cell sample carousel that houses four classes of vacuum-deposited organic thin films: polycyclic aromatic hydrocarbon (PAH), amino acid, metalloporphyrin, and quinone. The organic films are enclosed in hermetically sealed sample cells that contain one of four astrobiologically relevant microenvironments. Results are reported in this paper for the first 309 days of the mission, during which the samples were exposed for ∼2210 h to direct solar illumination (∼1080 kJ/cm(2) of solar energy over the 124-2600 nm range). Transmission spectra (200-1000 nm) were recorded for each film, at first daily and subsequently every 15 days, along with a solar spectrum and the dark response of the detector array. Results presented here include eight preflight and 16 in-flight spectra of eight SEVO sample cells. Spectra from the PAH thin film in a water-vapor-containing microenvironment indicate measurable change due to solar irradiation in orbit, while three other nominally water-free microenvironments show no appreciable change. The quinone anthrarufin showed high photostability and no significant spectroscopically measurable change in any of the four microenvironments during the same period. The SEVO experiment provides the first in situ real-time analysis of the photostability of organic compounds and biomarkers in orbit.


AIAA SPACE 2007 Conference & Exposition | 2007

Small Spacecraft for Science and Exploration in NASA's Robotic Lunar Exploration Program

Gregory Delory; Robert P. Lin; John W. Hines; Bruce Yost

NASA’s Vision for Space Exploration (VSE) calls for returning humans to the Moon as preparation for the exploration of Mars and the solar system. Robotic precursor missions can and should play an important role in our plans for a permanent human presence on the Moon. Here we describe a rationale and methodology for the development of small, low-cost, high-heritage lunar orbiters capable of measurements relevant to both basic lunar science and VSE priorities. The use of small (<150 kg) spacecraft enables a multitude of primary and secondary launch opportunities, and many small satellite systems developed for terrestrial applications are also applicable to lunar exploration. We show an example of one such lunar mission, based on a medium-class explorer spacecraft bus, which has been fully studied and costed as a primary or secondary payload and is capable of carrying out high priority measurements of the lunar environment. In order for small spacecraft to work effectively as part of a larger lunar exploration architecture, their design would ideally evolve toward a common bus, thus enabling repeatable and reliable access to lunar orbit. We summarize these and other possibilities explored during a summer study program involving students and the NASA Ames Research Center small satellite program, where we show that important lunar science and exploration objectives can be obtained using small spacecraft and for under


Acta Astronautica | 2014

The O/OREOS mission—Astrobiology in low Earth orbit

Pascale Ehrenfreund; Antonio J. Ricco; David Squires; Christopher Kitts; Elwood Agasid; Nathan Earl Bramall; Kathryn L. Bryson; Julie Diane Chittenden; Catharine A. Conley; Amanda Cook; Rocco L. Mancinelli; Andrew Mattioda; Wayne L. Nicholson; Richard C. Quinn; Orlando Santos; G. Tahu; M. Voytek; Chris Beasley; Laura Bica; Millan Diaz-Aguado; Charlie Friedericks; Mike Henschke; David Landis; Ed Luzzi; Diana Ly; Nghia Mai; Giovanni Minelli; Mike McIntyre; Michael Neumann; Macarena Parra

100M total cost.


Archive | 2007

Flight Results from the GeneSat-1 Biological Microsatellite Mission

Christopher Kitts; Karolyn Ronzano; Richard Rasay; Ignacio Mas; Phelps Williams; Paul Mahacek; Giovanni Minelli; John W. Hines; Elwood Agasid; Charlie Friedericks; Matthew Piccini; Macarena Parra; Linda Timucin; Christopher Beasley; Mike Henschke; Ed Luzzi; Nghia Mai; Mike McIntyre; Robert Ricks; David Squires; Chris Storment; John Tucker; Bruce Yost; Greg Defouw; Antonio J. Ricco


TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference | 2007

Autonomous Genetic Analysis System to Study Space Effects on Microorganisms: Results from Orbit

Antonio J. Ricco; John W. Hines; Matthew Piccini; Macarena Parra; Linda Timucin; V. Barker; Chris Storment; Charlie Friedericks; Elwood Agasid; Chris Beasley; Laurent Giovangrandi; Mike Henschke; Christopher Kitts; L. Levine; Ed Luzzi; Diana Ly; Ignacio Mas; M. Mclntyre; D. Oswell; Richard Rasay; Robert Ricks; Karolyn Ronzano; David Squires; G. Swaiss; John Tucker; Bruce Yost


Archive | 2010

O/OREOS Nanosatellite: A Multi-Payload Technology Demonstration

Giovanni Minelli; Antonio J. Ricco; Christopher Beasley; John W. Hines; Elwood Agasid; Bruce Yost; David Squires; Charlie Friedericks; Matthew Piccini; Greg Defouw; Mike McIntyre; Robert Ricks; Macarena Parra; Millan Diaz-Aguado; Linda Timucin; Mike Henschke; Matthew P. Lera; Ming Tan; Mike Cohen; Karolyn Ronzano; Ed Luzzi; Nghia Mai; Aaron Schooley; Dianna Ly; Eric Stackpole; Jeffrey Lin; John Tucker; Pascale Ehrenfreund; Nathan Santos; Bramall


Archive | 2005

The GeneSat-1 Test Demonstration Project: A Unique use of Smallsats

Bruce Yost; John W. Hines; Elwood Agasid; David Engelbert; Antonio J. Ricco; Christopher Kitts


Archive | 2008

Extended Life Flight Results from the GeneSat-1 Biological Microsatellite Mission

Giovanni Minelli; Christopher Kitts; Karolyn Ronzano; Christopher Beasley; Richard Rasay; Igancio Mas; Phelps Williams; Paul Mahacek; John Shepard; Jose Acain; John W. Hines; Elwood Agasid; Charlie Friedericks; Matthew Piccini; Macarena Parra; Linda Timucin; Mike Henschke; Ed Luzzi; Nghia Mai; Mike McIntyre; Robert Ricks; David Squires; Chris Storment; John Tucker; Bruce Yost; Greg Defouw; Antonio J. Ricco


Microfluidics, BioMEMS, and Medical Microsystems IX | 2011

PharmaSat: drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite

Antonio J. Ricco; Macarena Parra; David W. Niesel; Matthew Piccini; Diana Ly; Michael R. McGinnis; Andrzej Kudlicki; John W. Hines; Linda Timucin; Chris Beasley; Robert Ricks; Michael McIntyre; Charlie Friedericks; Michael Henschke; Ricky Leung; Millan Diaz-Aguado; Christopher Kitts; Ignacio Mas; Mike Rasay; Elwood Agasid; Ed Luzzi; Karolyn Ronzano; David Squires; Bruce Yost

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Ed Luzzi

Ames Research Center

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