Bruhaspathy Miriyala

Biocatalysis of the Antimalarial Artemisinin by Mucor ramannianus. Strains

Abstract Biocatalysis of artemisinin by different strains of Mucor ramannianus.* illustrates a simple approach to bioengineering for attaining useful biotransformation strains for preparative or downstream development. Three different strains of M. ramannianus. on the malt/sucrose medium were compared for their native ability to metabolize the antimalarial artemisinin and produce significant yields of hydroxylated artemisinin derivatives. The M. ramannianus. strains transformed the antimalarial into hydroxylated metabolites, in particular 7β.-hydroxyartemisinin at 88% yield and 6β.-hydroxyartemisinin at 51% yield. M. ramannianus. strains: M. ramannianus. 1839, University of Wisconsin, M. ramannianus. ATCC 9624, American Typical Cultures Collection, M. ramannianus. ATCC MYA-883 (R-56), Jefferson County, Arkansas (American Typical Cultures Collection).

Fluorous Methods for the Synthesis of Peptides and Oligonucleotides

The non-covalent affinity of a perfluoro chain towards similar has been exploited by many to separate fluorous tagged compounds from non-fluorous compounds by F-SPE or F-LLE. This purification strategy found its application across diverse fields including peptide and oligonucleotide synthesis where even slight inefficient couplings result in deletion sequences that are often difficult to remove from the target sequence. Two commonly employed strategies to address this problem involve end-tagging the target sequence or capping the deletion sequences with fluorous tags. Solution phase syntheses using soluble fluorous supports are easier and quicker. These approaches are reviewed here in detail.