Bruna de Azevedo Baêta

Influence of the physiological state on infestation by Rhipicephalus microplus in dairy cows

The purpose of this study was to identify the influence of physiological state, season, breed, number of lactations, dairy productivity, and stocking rate on infestation by Rhipicephalus microplus in dairy cows. Two hundred cows were selected through proportional representative sampling at the Seropédica Experimental Station (Pesagro-Rio), Brazil. Fully or partially engorged R. microplus females measuring between 4.5 and 8.0mm were counted on the right side of each animal. The tick infestation prevalence data were analyzed in relation to the risk factors, using multiple logistic regression. Associations between prevalence and its possible influencing determinants were measured using odds ratios. The average tick count of cows during the peripartum and lactation periods was significantly higher (p<0.05; OR=4.82) than the count in dry cows. Taurine animals showed significantly higher infestation (p<0.05; OR=3.28) than pure zebuine animals. Among cross-bred animals, the infestation was higher (p<0.05) in F1 animals (1/2 taurine×1/2 zebuine) than in Girolando animals (5/8 zebuine×3/8 taurine). Primiparous cows (p<0.05) had significantly heavier infestations than multiparous cows. Dairy production showed a positive correlation with tick infestation (p<0.03; OR=2.94), such that the most productive animals were the most parasitized ones. Animals kept at high densities were significantly more heavily infested (p<0.05, OR=6.32) than animals kept at low density. First-lactation and high-productivity taurine animals were more vulnerable to R. microplus, thus comprising a high-risk group in dairy herds.

Characterization of two strains of Anaplasma marginale isolated from cattle in Rio de Janeiro, Brazil, after propagation in tick cell culture

IDE8 tick cell cultures have been used for the isolation and propagation of several isolates of Anaplasma marginale. The genetic heterogeneity of A. marginale strains in cattle is diverse in endemic regions worldwide and the analyses of msp1α (major surface protein 1 alpha) gene sequences have allowed the identification of different strains. This study reports the isolation and propagation of two new isolates of A. marginale in IDE8 cells from blood of two cattle and their morphological and molecular characterization using light microscopy and the msp1α gene, respectively. Small colonies were observed in cytospin smears of each of the isolates 60 days after culture initiation. Based on msp1α sequence variation, the two isolates were found to be separate strains and were named AmRio1 and AmRio2. Analysis of msp1α microsatellite in both strains resulted in a single genotype, genotype E. The amino acid sequence of one MSP1α tandem repeat from the strain AmRio1 resulted in a new sequence (named 162) with one amino acid change. The results of these phylogenetic analyses demonstrated that A. marginale strains from Brazil and Argentina formed two large clusters of which one was less divergent that the other.

Use of plastic tips in artificial feeding of Dermacentor (Anocentor) nitens females Neumann, 1897 (Acari: Ixodidae).

The establishment of laboratory colonies of ticks is often hampered by their lack of adaptation to alternative hosts. The aim of this study was to artificially feed partially engorged Dermacentor (Anocentor) nitens females through plastic tips, and to identify what are the optimal conditions of application of this technique to get as much as possible close to the natural conditions. The technique of artificial feeding through plastic tips allowed the engorgement of D. nitens ticks to a final weight within the normal range for the species.

Risk factors relating to helminth infections in cows during the peripartum

The aim of this study was to investigate whether season, lactation number, breed standard and milk production were risk factors relating to occurrences of gastrointestinal nematodes in dairy cows during the peripartum period. Eighty-four cows were randomly selected through proportional stratified sampling. In order to analyze the fecal egg per gram (EPG) count, the data were subjected to the Spearman test, Kruskal-Wallis test at 5% significance and linear regression. At the time of calving, the cows showed high EPG counts in relation to all variables analyzed. Among the animals studied, we observed that purebred Holstein cows at their first lactation and with high milk production showed high EPG counts (600) and comprised the group most at risk within the herd studied. In this group, the animals showed moderate EPG during the prepartum period (300) and a significant increase (p < 0.01) in EPG count from the time of calving (900), i.e. an increase of the order of 300%. Selection of animals for milk production in tropical countries should be based not only on productive potential, but also on adaptive features.

Experimental infection of Rickettsia parkeri in the Rhipicephalus microplus tick

This study aimed to evaluate, by means of artificial feeding, the interaction between a pathogenic rickettsia and the hard tick R. microplus. We used partially engorged females fed on calves free of Rickettsia spp. Group 1 (G1), containing 20 ticks, was fed bovine blood only. Group 2 (G2), containing 20 ticks, was fed blood containing uninfected VERO cells, and group 3 (G3), containing 40 ticks, was fed blood containing VERO cells infected with Rickettsia parkeri. Biological parameters of the non-parasitic phase and a possible bacterial transmission to the tick eggs and to guinea pigs were evaluated. At the end of oviposition, all G3 females were PCR-positive for genes specific for the genus Rickettsia. Although no guinea pigs were infected, the experimental infection of R. microplus by R. parkeri caused a deleterious effect on the oviposition and provided the first report of transovarian transmission of rickettsia in this tick.

Alterações bioquímicas, anatômicas e histopatológicas em fígado de Gallus gallus Linnaeus, 1758 experimentalmente infectados por Borrelia anserina Sakharoff, 1891

A espiroquetose aviaria e uma enfermidade septicemica de curso agudo, cosmopolita, que acomete diversas especies aviarias, causada por Borrelia anserina e transmitida pelo carrapato Argas miniatus. O experimento teve como objetivos avaliar as alteracoes bioquimicas e anatomo-histopatologicas no figado de Gallus gallus, causadas pela infeccao experimental por B. anserina. Quarenta aves da especie G. gallus foram divididas em quatro grupos inteiramente casualizados com 10 animais cada: G1 - inoculado com soro infectado com B. anserina; G2 - inoculado com soro fisiologico a 0,9%; G3 - exposto a ninfas de terceiro instar de A. miniatus infectados por B. anserina; G4 - exposto a ninfas de terceiro instar de A. miniatus livres de B. anserina. As aves dos Grupos 1 e 3 manifestaram no 3o e 6o dias pos-inoculacao (DPI) respectivamente, sintomatologia caracteristica da doenca como inapetencia, perda de peso, sonolencia, diarreia esverdeada, mucosas hipocoradas, penas arrepiadas e hipertermia. Os niveis de ALT do Grupo 1 mostraram-se significativamente mais elevados apenas no 12oDPI e 24oDPI em relacao ao seu grupo controle (Grupo 2) e no Grupo 3 esses niveis se mantiveram elevados ate o 20o DPI em comparacao ao seu grupo controle (Grupo 4). Os niveis da enzima AST pouco oscilaram nos grupos experimentais, embora tenham sido encontradas elevacoes no 12oDPI nos Grupos 1 e 3. Os figados das aves dos Grupos 1 e 3 apresentaram a necropsia, moderada hepatomegalia, congestao, superficie irregular e coloracao vermelha a cianotica; constataram-se ainda pequenos pontos esbranquicados na superficie. A histopatologia do figado revelou congestao, infiltrados inflamatorios mononucleares, focos de necrose fibrinoide, dilatacao dos sinusoides e vacuolizacao de hepatocitos. A coloracao de Warthin-Starry revelou, nos figados das aves dos Grupos 1 e 3, a presenca de espiroquetas compativeis com B. anserina, frequentemente no interior de vasos sanguineos.