Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Bruno Pasquis is active.

Publication


Featured researches published by Bruno Pasquis.


Acta Ophthalmologica | 2012

Steady-state levels of retinal 24S-hydroxycholesterol are maintained by glial cells intervention after elevation of intraocular pressure in the rat.

Cynthia Fourgeux; Lucy Martine; Bruno Pasquis; Marie-Annick Maire; Niyazi Acar; Catherine Creuzot-Garcher; Alain M. Bron; Lionel Bretillon

Purpose:  Our previous studies suggested that CYP46A1 and 24S‐hydroxycholesterol (24SOH) may be associated with glaucoma. Loss of CYP46A1‐expressing retinal ganglion cells is involved in the activation of glia, and therefore possibly in the disbalance of cholesterol. In this context, the purpose of our present work was to emphasize the glial and longitudinal CYP46A1 expression after an interventional glaucoma‐related stress triggered by elevated intraocular pressure (IOP).


Laryngoscope | 2013

Oral homeostasis disruption by medical plasticizer component bisphenol A in adult male rats.

Mireille Folia; Sofiane Boudalia; Franck Ménétrier; Laurence Decocq; Bruno Pasquis; Charles Schneider; Raymond Berges; Yves Artur; Marie-Chantal Canivenc-Lavier

Bisphenol A (BPA) is a synthetic estrogen‐like chemical mimetic widely used in the manufacture of polycarbonate plastics and epoxy resins found in numerous consumer products including food packaging, medical devices, and dental sealants. Because it is recovered in fluids and it can reach high levels in saliva, this study aimed to evaluate its safety on oral homeostasis by examining its effects on salivary glands, mouth epithelium, water consumption, and salt preference, each parameter being estrogen sensitive.


Experimental Eye Research | 2015

Early adaptive response of the retina to a pro-diabetogenic diet: Impairment of cone response and gene expression changes in high-fructose fed rats.

Magalie Thierry; Bruno Pasquis; Bénédicte Buteau; Cynthia Fourgeux; Doulaye Dembélé; Laurent Leclère; Ségolène Gambert-Nicot; Niyazi Acar; Alain M. Bron; Catherine Creuzot-Garcher; Lionel Bretillon

The lack of plasticity of neurons to respond to dietary changes, such as high fat and high fructose diets, by modulating gene and protein expression has been associated with functional and behavioral impairments that can have detrimental consequences. The inhibition of high fat-induced rewiring of hypothalamic neurons induced obesity. Feeding rodents with high fructose is a recognized and widely used model to trigger obesity and metabolic syndrome. However the adaptive response of the retina to short term feeding with high fructose is poorly documented. We therefore aimed to characterize both the functional and gene expression changes in the neurosensory retina of Brown Norway rats fed during 3 and 8 days with a 60%-rich fructose diet (n = 16 per diet and per time point). Glucose, insulin, leptin, triacylglycerols, total cholesterol, HDL-cholesterol, LDL-cholesterol and fructosamine were quantified in plasma (n = 8 in each group). Functionality of the inner retina was studied using scotopic single flash electroretinography (n = 8 in each group) and the individual response of rod and cone photoreceptors was determined using 8.02 Hz Flicker electroretinography (n = 8 in each group). Analysis of gene expression in the neurosensory retina was performed by Affymetrix genechips, and confirmed by RT-qPCR (n = 6 in each group). Elevated glycemia (+13%), insulinemia (+83%), and leptinemia (+172%) was observed after 8 days of fructose feeding. The cone photoreceptor response was altered at day 8 in high fructose fed rats (Δ = 0.5 log unit of light stimulus intensity). Affymetrix analysis of gene expression highlighted significant modulation of the pathways of eIF2 signaling and endoplasmic reticulum stress, regulation of eIF4 and p70S6K signaling, as well as mTOR signaling and mitochondrial dysfunction. RT-qPCR analysis confirmed the down regulation of Crystallins, Npy, Nid1 and Optc genes after 3 days of fructose feeding, and up regulation of End2. Meanwhile, a trend towards an increased expression of αA- and αB-crystallin proteins was observed at day 8. Our results are consistent with early alterations of the functioning and gene expression in the retina in a pro diabetogenic environment.


PLOS ONE | 2014

Metabolic syndrome triggered by high-fructose diet favors choroidal neovascularization and impairs retinal light sensitivity in the rat

Magalie Thierry; Bruno Pasquis; Niyazi Acar; Stéphane Grégoire; Valérie Febvret; Bénédicte Buteau; Ségolène Gambert-Nicot; Alain M. Bron; Catherine Creuzot-Garcher; Lionel Bretillon

Diabetic retinopathy and age-related macular degeneration are the leading causes of blindness in Western populations. Although it is a matter of controversy, large-scale population-based studies have reported increased prevalence of age-related macular degeneration in patients with diabetes or diabetic retinopathy. We hypothesized that metabolic syndrome, one of the major risk factors for type 2 diabetes, would represent a favorable environment for the development of choroidal neovascularization, the main complication of age-related macular degeneration. The fructose-fed rat was used as a model for metabolic syndrome in which choroidal neovascularization was induced by laser photocoagulation. Male Brown Norway rats were fed for 1, 3, and 6 months with a standard equilibrated chow diet or a 60%-rich fructose diet (n = 24 per time point). The animals expectedly developed significant body adiposity (+17%), liver steatosis at 3 and 6 months, hyperleptinemia at 1 and 3 months (two-fold increase) and hyperinsulinemia at 3 and 6 months (up to two-fold increase), but remained normoglycemic and normolipemic. The fructose-fed animals exhibited partial loss of rod sensitivity to light stimulus and reduced amplitude of oscillatory potentials at 6 months. Fructose-fed rats developed significantly more choroidal neovascularization at 14 and 21 days post-laser photocoagulation after 1 and 3 months of diet compared to animals fed the control diet. These results were consistent with infiltration/activation of phagocytic cells and up-regulation of pro-angiogenic gene expression such as Vegf and Leptin in the retina. Our data therefore suggested that metabolic syndrome would exacerbate the development of choroidal neovascularization in our experimental model.


Audiology and Neuro-otology | 2016

Implantation of the Auditory Nerve via the Middle Ear Cavity in Rats with Partial Hearing Preservation

Caroline Guigou; G. Leterme; Bruno Pasquis; Laurent Martin; Guillaume Tourrel; Lionel Bretillon; Alexis Bozorg Grayeli

The aim of this study was to evaluate the residual hearing function and cochlear morphology after auditory nerve implantation via middle ear spaces in rats. A titanium rod (1.5 mm long and 0.3 mm thick) coated with Parylene was inserted in the cochlear apex in the direction of the modiolus in 9 Wistar rats. Auditory brainstem-evoked responses to tone bursts at 2, 8, 12 and 32 kHz were recorded before surgery and on postoperative days 0, 2, 15 and 30. Eight cochleas were examined microscopically. The rod was inside the modiolus in 4, and partly or totally outside the modiolus in 4 animals. Residual hearing was present in all cases. The average threshold shift in cochleas with modiolar implant was 39 ± 11.2, 54 ± 9.7, 48 ± 20.3 and 43 ± 21.3 dB SPL on postoperative days 0, 2, 15 and 30, respectively. The transmodiolar approach allows a minimally invasive cochlear implantation and a partial hearing preservation.


Journal Francais D Ophtalmologie | 2007

042 Effets de colorants intravitréens sur l’électrorétinogramme chez le rat

A. Guillaubey; Niyazi Acar; S. Bidot; A. Borey; Bruno Pasquis; L. Malvitte; Lionel Bretillon; A. Bron; Catherine Creuzot-Garcher

Introduction Le vert d’indocyanine (ICG) et le bleu trypan (BT) sont couramment utilises comme marqueurs colores dans la chirurgie maculaire pour faciliter l’ablation de la limitante interne. L’innocuite de ces colorants dans le segment posterieur n’est a ce jour pas etablie et certains auteurs ont propose l’utilisation d’acetonide de triamcinolone (AT) comme alternative. Le but de cette etude a ete d’evaluer les effets de ces trois differentes substances injectees dans le vitre sur la fonction retinienne evaluee par electroretinographie chez le rat. Materiels et Methodes Trois groupes de six rats Sprague Dawley âges de 12 semaines ont recu une injection intravitreenne de 0,1 ml de colorant dans l’œil droit (0,5 mg/mL d’ICG, 3 mg/mL de BT ou 40 mg/mL d’AT) suivi par un rincage avec 1 ml de solution saline a pression constante avec evacuation du liquide par une seconde sclerotomie. L’œil gauche a subi uniquement l’injection de la solution saline selon le meme protocole. Un electroretinogramme (ERG) de type scotopique a trois intensites lumineuses (10 mcds/m2, 2500 mcds/m2 et 25000 mcds/m2) a ete enregistre sur chaque œil avant traitement et 28 jours apres l’intervention. Resultats Aucun effet du traitement n’a ete observe sur l’ERG des yeux temoins et les yeux traites au BT et AT. L’amplitude et le temps de latence de l’onde b de l’ERG ont ete significativement diminues dans les yeux traites avec l’ICG a 10mcds/m2 et 2500 mcds/m2 mais pas a 25000 mcds/m2 (amplitudes moyennes : t0 = 174,8 μV versus t28 = 55,0 μV a 10mcds/m2 p Discussion Cette etude montre que qu’une exposition meme passagere de la retine a l’ICG peut avoir des effets sur la fonction retinienne. Conclusion Aussi l’utilisation de l’AT et du BT pourrait etre une alternative satisfaisante dans la pratique de la chirurgie maculaire.


Journal Francais D Ophtalmologie | 2007

008 Évaluation d’un modèle de glaucome induit par laser chez le rat

A. Bron; C. Schnebelen; M. Salinas-Navarro; Niyazi Acar; S. Arnavielle; Bruno Pasquis; Catherine Creuzot-Garcher; M. Vidal-Sanz; Lionel Bretillon

Introduction Evaluer chez un modele de glaucome chez le rat induit par photocoagulation au laser, l’evolution de la pression intraoculaire (PIO), la fonction retinienne par electroretinographie et l’alteration des cellules ganglionnaires par le marquage retrograde. Materiels et Methodes Les experiences ont ete menees sur des rats Sprague Dawley mâle âges de six semaines. Trois des veines episclerales, le trabeculum et le limbe ont ete photocoagules sur un seul œil avec un laser a 532 nm. La PIO a ete mesuree toutes les deux semaines pendant onze semaines de meme que l’electroretinogramme (ERG) de type scotopique. Trois mois apres le traitement au laser, la survie des cellules ganglionnaires a ete evaluee par l’intermediaire d’un marquage retrograde du colliculus par du fluorogold a 5 %. Les cellules ganglionnaires viables ont ete comptees par systeme d’analyse d’images. Resultats La PIO a ete augmentee de facon statistiquement significative dans l’œil traite de chaque animal des la premiere semaine apres traitement (32 mmHg contre 18 mmHg dans les yeux non traites) et ceux pendant 7 semaines. L’amplitude de l’onde b a l’ERG a diminue d’environ 50 % par rapport a l’œil controle 11 semaines apres la photocoagulation. Nous n’avons pas note de differences dans les temps latence de l’onde b de l’ERG. Une diminution de 30 % du nombre des cellules ganglionnaires a ete observee dans les yeux traites par rapport aux yeux temoins. Discussion De nombreux modeles de glaucome ont ete developpes. La difficulte consiste a disposer des parametres fonctionnels (ERG) et structuraux (nombre des cellules ganglionnaires) de facon simultanee. Conclusion Ce travail demontre la validite de ce modele de glaucome induit par photocoagulation au laser chez le rat.


Neurotoxicology and Teratology | 2014

A multi-generational study on low-dose BPA exposure in Wistar rats: effects on maternal behavior, flavor intake and development.

Sofiane Boudalia; Raymond Berges; Claire Chabanet; Mireille Folia; Laurence Decocq; Bruno Pasquis; Latifa Abdennebi-Najar; Marie-Chantal Canivenc-Lavier


La Revue Française de Gériatrie et de Gérontologie | 2012

Effects of oral Bisphenol A exposure on sweet preference and on submandibular salivary gland structure in male adult rat

Mireille Folia; Sofiane Boudalia; Franck Ménétrier; Laurence Decocq-Poumot; Bruno Pasquis; Charles Schneider; Raymond Berges; Yves Artur; Marie-Chantal Canivenc-Lavier


PLOS ONE | 2014

Laser-induced choroidal neovascularization (CNV) is enhanced in rats fed with a 60%-rich fructose diet.

Magalie Thierry; Bruno Pasquis; Niyazi Acar; Stéphane Grégoire; Valérie Febvret; Bénédicte Buteau; Ségolène Gambert-Nicot; Alain M. Bron; Catherine Creuzot-Garcher; Lionel Bretillon

Collaboration


Dive into the Bruno Pasquis's collaboration.

Top Co-Authors

Avatar

Lionel Bretillon

Centre national de la recherche scientifique

View shared research outputs
Top Co-Authors

Avatar

Catherine Creuzot-Garcher

Institut national de la recherche agronomique

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Marie-Chantal Canivenc-Lavier

Institut national de la recherche agronomique

View shared research outputs
Top Co-Authors

Avatar

Raymond Berges

Institut national de la recherche agronomique

View shared research outputs
Top Co-Authors

Avatar

Sofiane Boudalia

Institut national de la recherche agronomique

View shared research outputs
Top Co-Authors

Avatar

Charles Schneider

Institut national de la recherche agronomique

View shared research outputs
Top Co-Authors

Avatar

Yves Artur

University of Burgundy

View shared research outputs
Top Co-Authors

Avatar

Bénédicte Buteau

Institut national de la recherche agronomique

View shared research outputs
Top Co-Authors

Avatar

Laurence Decocq

Institut national de la recherche agronomique

View shared research outputs
Researchain Logo
Decentralizing Knowledge