Bui Chi Trung

STEM NODULATION ON Aeschynomene indica PLANTS BY ISOLATED RHIZOBIA

Stem nodulation on Aeschynomene indica was first reported 30 years ago (l). The nodule have been observed only on the submerged lower parts of stems. Recently, this species has been found to bear nodules on the aerial parts of stems under natural conditions, which fix dinitrogen as indicated by their acetylene reducing activities (7). EAGLESHAM and SZALAY (3) reported more recently that a single inoculation of the stems of Aeschynomene plants with a rhizobial strain isolated from a submerged stem nodule on an A. indica induced the formation of green N2-fixing aerial stem nodules. However, the mechanism of this stem nodulation had remained unclear. The objectives of this study were: (a) to compare the ability of stem nodulation between rhizobial strains isolated from root and stem nodules; and (b) to examine the effects of sucrose and yeast extract on stem nodulation.

Improvement of Leonard jar assembly for screening of effective rhizobium

Abstract Leonard jar assembly (1) which is a useful apparatus for aseptic culture of the underground part of plant, has been used for the study of legume-rhizobium symbiosis. However, the assembly has some disadvantages requiring improvement (2-4). One of the disadvantages is the tendency to restrict root growth in the growth bottle, which hinders nodule formation and nitrogen fixation of leguminous plants. In this paper, an improvement of Leonard jar assembly is reported and also nodulation and nitrogen fixation of mungbean grown with the improved jar assembly were investigated in relation to the effectiveness of rhizobium inoculation.

A COMMENT ON THE VARIETAL DIFFERENCES OF PRODUCTION OF MUNGBEAN AND ITS GRAIN PROPERTIES

Abstract Mungbean (Vigna radiata) is one of the important leguminous crops in tropical and temperate regions and its grain is utilized as sources of protein and carbohydrate in several countries, especially in tropical ones (3, 4). Moreover, it is also imported into Japan as the material of be an sprouts and so on.