Byeong-Ju Baik

Histamine Release Induced By Dendroaspis Natriuretic Peptide From Rat Mast Cells

Dendroaspis natriuretic peptide (DNP), recently isolated from the venom of the green Mamba snake Dendroaspis angusticeps, is a 38 amino acid peptide containing a 17 amino acid disulfide ring structure similar to that of the natriuretic peptide family. The natriuretic peptide family is known to induce histamine release from human and rat mast cells, but there are no published data concerning the effects of DNP on histamine release from mast cells. The purpose of this study is to investigate whether DNP induces the histamine release from rat peritoneal mast cells (RMPCs) and to determine the mechanism of DNP-induced histamine release from RPMCs. After treatment of RPMC with DNP, mast cell degranulation was observed, and calcium uptake and histamine release were measured. DNP released the histamine, induced the mast cell degranulation, and increased the calcium uptake of RPMCs, in a dose-dependent manner. The results indicate that DNP can increase Ca-uptake and induce histamine release.

Rapid Detection of S. Mutans Surface Antigen I/II Using a Sensitive Monoclonal Anti-Ag I/II Antibody by ELISA

The cell-surface protein antigen I/II (Ag I/II) is expressed in oral streptococci, which are known as the causative agent of a number of diseases including dental caries, endocarditis, gingivitis, and periodontal disease. Consequently, monoclonal antibodies (MAb) capable of recognizing the streptococcal Ag I/II protein could be a useful tool for the diagnosis and cure of these diseases. In this study, a previously generated monoclonal anti-Ag I/II antibody, ckAg I/II, was used to detect a small amount of Streptococcus mutans (S. mutans) surface antigen Ag I/II. The ckAg I/II was proved to be very sensitive and able to detect as little as 1 ng of recombinant Ag I/II protein within 5 min and Ag I/II in saliva within 10 min, as well as native Ag I/II in 20 μL of culture supernatant by ELISA. These results suggest that ckAg I/II can be used as a fast and efficient diagnostic tool to detect Ag I/II.

The Distribution and Treatment of Outpatients with General Anesthesia in Chonbuk National University Dental Hospital for 9 Years

General anesthesia (GA) for dental care in handicapped patients is necessary to facilitate the provision of safe, efficient, and effective quality treatment. The aims of this study were to determine the anesthetic characteristics of handicapped patients in need of dental treatment in these day care units, and to establish for plan to provide better services. 325 patients who had outpatient general anesthesia from January 2005 to March 2014 were assessed for this study. Patients’distribution and treatment patterns were examined. The proportion of male patients (202, 62.2%) was higher than female patients (123, 37.8%) and the largest group of patients were 5 to 10 years old (85, 26.2%). The reasons for general anesthesia included mental and physical disabilities (207, 63.7%), behavior management (84, 25.8%), parent needs (14, 4.3%), and so on. Restorative treatment was the most common procedure with the average of 4.2 teeth treated per one patient and 43 (13.2%) patients underwent general anesthesia for dental treatment more than once. To expand and improve access to the dental care of the disabled, improvement of the health care system, enhancement of their training for dental care by professionals, and enlarging caregivers’understanding of the importance of oral care in the early stages are required.

PULP REVASCULARIZATION OF IMMATURE FIRST PERMANENT MOLARS WITH APICAL PERIODONTITIS : CASE REPORT

Revascularization of the pulp in a necrotic, infected immature tooth with apical periodontitis was attempting several years. Revascularization of partially necrotic pulp in an immature tooth is based on the concept that vital dental stem cells can survive pulpal necrosis. Revascularization procedure obtains longer and thicker roots in teeth with necrotic pulp diagnosis. Pulp revascularization for immature permanent molars can be possibly applied on cases having difficulty to use conventional root canal treatment due to abnormally thin root canal wall or severe root curvature. Also, when an uncooperative patient does not agree with sedative treatment the revascularization can be useful. And a patient with disability who is barely cooperative can be another indication of this treatment. In this case report, pulp revascularization using triple-antibiotics, metronidazole, ciprofloxacine and minocycline, was applied on the immature first permanent molar infected by caries.

Detection of Streptococcus mutans in Saliva using Monoclonal Antibodies

본 연구의 목적은 Streptococcus mutans의 Ag I/II와 glucosyltransferase에 대해 개발된 단일클론항체를 사용한 타액 내 Streptococcus mutans 검출의 효용성을 평가하기 위함이다. 세 군(성인, 미성년자, 그리고 교정치료중인 미성년자)을 대상으로 DMFT,

Maxillary Labial Frenum and Its Relationship to Developing Dentition in Korean Children

Dentocult^{(R)}

Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method

-SM strip mutans를 이용한 타액내의 colony density (CFU/mL), polymerase chain reaction을 이용한 Sreptococcus mutans와 Sreptococcus sobrinus의 존재여부, 그리고 4가지 단일클론항체를 사용한 타액 내 Streptococcus mutans 검출 실험을 시행하였다. 그 결과 Streptococcus mutans가 Streptococcus sobrinus보다 치아우식 형성에 더 주요하게 작용한다는 것을 입증하였으며, glucosyltransferases (gtf B, gtf C, gtf D)와 Ag I/II에 대한 단일클론항체들의 Streptococcus mutans를 검출해 내는 능력이

Prevalence and Distribution of Congenitally Missing Teeth in Patients Visiting the Department of Pediatric Dentistry of Chonbuk National University Hospita

Dentocult^{(R)}

EFFECTS OF MOUTH BREATHING ON FACIAL SKELETAL MORPHOLOGY

-SM strip mutans나 polymerase chain reaction을 사용한 검출법보다 뛰어남을 보였다. 【The purpose of this study was to determine the usefulness of a detection method for Streptococcus mutans in saliva with monoclonal antibodies developed targeting Ag I/II and glucosyltransferases (gtf B, gtf C and gtf D) in Streptococcus mutans. In the three groups tested (adults, minors, and minors under orthodontic treatment), the results of the DMFT scores, the colony density (CFU/mL) in their saliva was measured using

DISTRIBUTION AND TRANSMISSION OF STREPTOCOCCUS MUTANS AMONG CHILDREN AND THEIR MOTHERS

Dentocult^{(R)}