C. Altucci

Thermal and nonthermal ion emission during high-fluence femtosecond laser ablation of metallic targets

We have investigated the emission of positive ions from metallic targets irradiated with intense, ultrashort laser pulses (≈120 fs) at 780 nm, in both S and P polarized states. The measured energy spectra show the presence of a nonthermal, high-energy (several keV) ion component accompanying low-energy ions (tens of eV) produced by a thermal mechanism. The yield of the high-energy component shows a strong dependence on both laser fluence and light polarization. For the low-energy component a higher ablation efficiency was observed for P polarization, and ascribed to a more effective absorption mechanism active during the laser–target interaction.

High harmonic generation spectroscopy of hydrocarbons

We have demonstrated the ability of few-cycle midinfrared intense laser pulses to produce extended harmonic spectra (≥45 eV) suitable for high harmonic spectroscopy in aligned hydrocarbons with ionization potentials in the range 9.07–11.52 eV. Modulations in the spectra measured with different alignment angles show signatures of the molecular structure. These results pave the way for the extension of high harmonic spectroscopy to complex biomolecules.

Detection of Parathion Pesticide by Quartz Crystal Microbalance Functionalized with UV-Activated Antibodies

Photonic immobilization technique (PIT) has been used to develop an immunosensor for the detection of parathion. An antibody solution has been activated by breaking the disulfide bridge in the triad Trp/Cys-Cys through absorption of ultrashort UV laser pulses. The free thiol groups so produced interact with gold lamina making the antibody oriented upside, that is, with its variable parts exposed to the environment, thereby greatly increasing the detection efficiency. PIT has been applied to anchor polyclonal antiparathion antibodies to the gold electrode of a Quartz Crystal Microbalance (QCM) giving rise to very high detection sensitivity once the parathion is made heavier by complexion with BSA (bovine serum albumin), this latter step only required by the mass based transducer used in this case. The comparison of the sensor response with irradiated antibodies against different analytes shows that the high degree of antibody specificity is not affected by PIT nor is it by the complexion of parathion with BSA. These results pave the way to important applications in biosensing, since the widespread occurrence of the Trp/Cys-Cys residues triads in proteins make our procedure very general and effective to detect light analytes.

Harmonic generation in gases by use of Bessel–Gauss laser beams

We have experimentally investigated the use of diffraction-free beams to generate low-order harmonics in gas jets. Bessel–Gauss beams have been generated from a Ti:sapphire, 100-fs laser by use of annular apertures and then by focusing of the ring-type–produced radiation. The conversion efficiencies for these beams for the third- and fifth-order harmonic generation in xenon have been measured and compared with those of pure Gaussian beams of the same energy content. We have thus found that, by matching the gas medium length to the range of the Bessel–Gauss beams, conversion efficiencies considerably higher than those of Gaussian beams can be obtained. The experimental results have also been discussed and interpreted in terms of a simple numerical model that emphasizes the effects of phase matching in the two different geometries.

Extension of high harmonic spectroscopy in molecules by a 1300 nm laser field

The emerging techniques of molecular spectroscopy by high order harmonic generation have hitherto been conducted only with Ti:Sapphire lasers which are restricted to molecules with high ionization potentials. In order to gain information on the molecular structure, a broad enough range of harmonics is required. This implies using high laser intensities which would saturate the ionization of most molecular systems of interest, e.g. organic molecules. Using a laser at 1300 nm, we are able to extend the technique to molecules with relatively low ionization potentials (approximately 11 eV), observing wide harmonic spectra reaching up to 60 eV. This energy range improves spatial resolution of the high harmonic spectroscopy to the point where interference minima in harmonic spectra of N(2)O and C(2)H(2) can be observed.

Light assisted antibody immobilization for bio-sensing

Ultrashort UV pulses at 258 nm with repetition rate of 10 kHz have been used to irradiate buffer solution of antibody. The tryptophan residues strongly absorb this radiation thus becoming capable to disrupt the disulfide bridges located next to them. Due to their high reactivity the opened bridges can anchor a gold plate more efficiently than other sites of the macromolecule giving rise to preferential orientations of the variable part of the antibody. UV irradiation has been applied to anchor antiIgG antibody to the electrode of a Quartz Crystal Microbalance (QCM) that lends itself as a sensor, the antibody acting as the bio-receptor. An increase of the QCM sensitivity and of the linear range has been measured when the antibody is irradiated with UV laser pulses. The photo-induced reactions leading to disulfide bridge breakage have been analyzed by means of a chemical assay that confirms our explanation. The control of disulfide bridges by UV light paves the way to important applications for sensing purpose since cysteine in combination with tryptophan can act as a hook to link refractory bio-receptors to surfaces.

Single attosecond light pulses from multi-cycle laser sources

High harmonic generation provides a means of producing attosecond pulses of light which are the shortest, controllable probes available to science for time-resolving ultrafast dynamics. We review techniques based on high harmonic generation for generating single attosecond pulses using high-power, multi-cycle laser sources, including optical-, polarisation-, and ionisation-gating schemes as well as techniques based on field synthesis. By significantly reducing the technical demands placed on the driving laser, these techniques have the potential to greatly broaden the application base for attosecond pulses.

Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase

A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0.17 mM) glucose determination with a detection range from 0.4 mM to 5 mM.

Interplay between group-delay-dispersion-induced polarization gating and ionization to generate isolated attosecond pulses from multicycle lasers

We implemented a new experimental scheme for the generation of single-shot extreme-UV continua that exploits a combination of transform-limited 15 fs, 800 nm pulses and chirped 35 fs, 800 nm pulses with orthogonal polarizations. Continua are interpreted as the formation of a single attosecond pulse and attributed to the interplay between polarization, ionization gating, and trajectory selection operated by suitable phase-matching conditions.

Measurement of the two-photon absorption cross-section of liquid argon with a time projection chamber

This paper reports on laser-induced multiphoton ionization at 266?nm of liquid argon in a time projection chamber (LAr TPC) detector. The electron signal produced by the laser beam is a formidable tool for the calibration and monitoring of next-generation large-mass LAr TPCs. The detector that we designed and tested allowed us to measure the two-photon absorption cross-section of LAr with unprecedented accuracy and precision: ?ex=(1.24?0.10stat?0.30syst)?10? 56?cm4?s? 1.