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Dive into the research topics where C. Dictus-Vermeulen is active.

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Featured researches published by C. Dictus-Vermeulen.


Clinica Chimica Acta | 1988

Alkaline phosphatase isoenzyme pattern in human amniotic fluid is dependent on the level of total activity. Implications in cystic fibrosis diagnosis.

C. Dictus-Vermeulen; J. Ameryckx; Christiane Gueuning; E. C. Van Bogaert; Guy Graff

Alkaline phosphatase activities have been examined in 500 consecutive human amniotic fluids obtained at diagnostic paracentesis between 14 and 38 wk of gestation. They were found to have a non-Gaussian, positively skewed distribution, independent of the protein concentrations. Residual activities after heat treatment or in presence of phenylalanine and levamisole allow evaluation of the placental, hepatic and intestinal isoenzyme components. It is shown that the contribution of the intestinal isoenzyme to the total activity is a linear function of the latter. This fact should be taken into account in the enzymatic diagnosis of cystic fibrosis as it is apparent that the part contributed by the intestinal isoenzyme is predictably smaller when the level of total activity is low.


Archives of Physiology and Biochemistry | 1978

Effect of increasing duration of denervation on the rate of entry of inorganic phosphate into rat gastrocnemius muscle.

Guy Graff; Christiane Gueuning; C. Dictus-Vermeulen

The increased inorganic phosphate flow, characteristic of denervated gastrocnemius muscle, is shown to have no direct relation with either the loss of muscle mass or with the concentrations of the acid-soluble phosphate fractions. It is shown to increase hyperbolically with the time elapsed since the nerve section. The asymptotic value reached after thirty days suggests the presence of a saturable mechanism.


Archives of Physiology and Biochemistry | 1989

Alkaline phosphatase activities in normal and denervated, slow and fast rat muscles. Bearing on the possible participation of the enzyme in the transmembrane transport of phosphate ions.

C. Dictus-Vermeulen; Christiane Gueuning; Guy Graff

Alkaline phosphatase (AP) activities are present in rat gastrocnemius:48.7, plantaris: 68.9, tibialis anterior: 69.1 and soleus: 96.7 nmol phenol. min-1. 100 mg muscles-1. These concentrations are one and two orders of magnitude lower than those observed in duodenum and placenta, but similar to those observed in liver. Response to activators/inhibitors and electrophoretic behaviour assign the muscle AP activities to the rat liver/placenta isoenzyme group. Motor denervation does not affect significantly the total muscle AP content within the first 30 postoperative days, however the concomitant variations in muscle weight are responsible for wide differences in AP concentrations between innervated, denervated and reinnervated muscles. Parallel determinations of radiophosphate uptake and AP activities failed to document a necessary link between the two variables, i.e. between enzyme concentration and phosphate ion transport.


Archives of Physiology and Biochemistry | 1978

Unlike Effects of Denervation on the Rate of Entry of Inorganic Phosphate Into Rat Slow and Fast Muscles

Guy Graff; Christiane Gueuning; C. Dictus-Vermeulen

The increased inorganic phosphate flow, characteristic of denervated gastrocnemius muscle is shown to be present in additional denervated fast muscles, i.e. the plantaris, tibialis anterior and extensor digitorum longus muscles. The response of the soleus, a slow muscle, to denervation is biphasic. After an initial decrease of the phosphate flow at the end of the first postoperative day, there is a secondary rise which has the same general characteristics as the rise observed in fast muscles i.e. an exponential or hyperbolic increase to an asymptotic value reached after thirty days. The denervated fast and slow muscles are not converging to an intermediate metabolic pattern. The changes in phosphate flow induced by denervation are reversible in the soleus as well as in the gastrocnemius muscles.


Archives of Physiology and Biochemistry | 1978

Effects of Denervation on the Rate of Entry of Inorganic Phosphate into Rat Slow and Fast Muscles: Selective Inhibition of Denervation Changes by Actinomycin D

Guy Graff; Christiane Gueuning; C. Dictus-Vermeulen

Actinomycin D abolishes the post denervation increase in inorganic phosphate flow observed in the fast gastrocnemius muscle. In the slow soleus muscle, the initial decrease in phosphate flow is unaffected but the secondary rise is suppressed in the same manner as in the fast muscle. These observations put the post denervation increase in inorganic phosphate flow on a par with the development of extrajunctional cholinergic receptors in being the result of the synthesis of new proteins. It has the added advantage of being suitable to quantitative assessment at the whole muscle level.


Archives of Physiology and Biochemistry | 1988

Alkaline phosphatase activities in human amniotic fluid, chromatographic separation of the foetal intestinal component

J. Ameryckx; C. Dictus-Vermeulen; Christiane Gueuning; E. C. Van Bogaert; Guy Graff

Hydrophilic gel permeation chromatography of 14-36 wk human amniotic fluid on Fractogel columns divides the total alkaline phosphatase (AP) activity in a higher and a lower mol wt zones. Differential inhibition testing, isoelectric focusing, cellulose acetate, agarose and polyacrylamide gel electrophoreses before and after neuraminidase treatment show the higher mol wt zone to be homogeneous and to be made of the higher mol wt foetal intestinal isoenzyme form whereas the lower mol wt zone represents an unresolved mixture of hepatic, placental and lower mol wt foetal intestinal isoenzymes. In the early stages of pregnancy, the activity associated with the higher mol wt zone outweighs by far that of the lower mol wt zone; however from the 24 th week one notes a steady increase in the relative magnitude of this second zone until at the end of the gestation period both zones assume near equal importance albeit within a lower total AP activity. Satisfactory quantitation of the higher mol wt foetal intestinal isoenzyme form in one ml amniotic fluid can be attained after a 3-h chromatography run using p-nitrophenylphosphate as substrate.


Archives of Physiology and Biochemistry | 1980

Early and late systemic effects of colchicine on muscle permeability to inorganic phosphate.

Guy Graff; Christiane Gueuning; C. Dictus-Vermeulen

A single systemic injection of 75 micrograms colchicine/100 g body weight in the lumbar muscles increases within 6 h the permeability of the extensor digitorum longus muscle to inorganic phosphate. Twenty four hours after the injection the specific activities and isotope uptakes of both inorganic and organic-bound acid-soluble phosphates are markedly increased. By the third day a maximal four-fold increase in rate of inorganic phosphate exchange is reached. The observed effects are slowly reversible, a near normal situation obtaining after 30 days. They are qualitatively different from those observed after administration of vincristine sulphate and bupivacaïne (Marcaine).


Archives of Physiology and Biochemistry | 1978

Neurotoxicité de l'acrylamide chez le rat

Christiane Gueuning; Guy Graff; C. Dictus-Vermeulen

AbstractThe subcutaneous injection of acrylamide (30 mg kg−1 day−1) in adult male rats induces a severe impairment of the general state of health and a progressive polyneuropathy at the cumulative dose of 180 mg/kg.At the cumulative dose of 400 mg acrylamide does not interfere with the incorporation of plasma inorganic phosphate into the inorganic and organic acid-soluble phosphate fractions of either the gastrocnemius muscle or the sciatic nerve Schwann cells. Nor does it modify the characteristic metabolic response of these fractions to Wallerian degeneration and neurogenic muscle atrophy.


Archives of Physiology and Biochemistry | 1977

Incorporation Du Radiophosphate Plasmatique Dans Les Fractions Phosphates Minéral, Organiques Acido-Solubles Et Phospholipidiques Des Nerfs Sciatiques De Lapin, Normal Et En Voie De Degénérescence Wallérienne

Christiane Gueuning; C. Dictus-Vermeulen; Guy Graff

AbstractInorganic phosphate exchanges between plasma and sciatic nerve have been measured in the rabbit using a 32PO4 tracer technique. Inorganic phosphate is taken up at the rate of 0.13 μg per hour and per 100 mg fresh weight.Incorporation of plasma radiophosphate is markedly increased into the inorganic and organic acid soluble phosphate fractions of the distal part of the sectioned sciatic nerve. This increase is already significant within one hour after surgical division, spreading at least 3 cm distally within 6 hours. This high level of incorporation persists until the 29th day of degeneration.These results favour the hypothesis that the axonal continuity maintains the metabolic activity of the Schwann cells at an inframaximal level. We confirm the rapid decrease in total phospholipid concentration in the nerve undergoing Wallerian degeneration as well as the marked increase in their specific activity. We show however that this increase in specific activity is due partly to the increased specific a...


Clinica Chimica Acta | 1980

Human placental oxytocinase and its relationship to pregnancy plasma oxytocinase

Henri Kleiner; C. Dictus-Vermeulen; Claude May-Cocriamont; Michèle Brouet-Yager; Anna Popowski; Roger Mosselmans; Guy Graff

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Guy Graff

Université libre de Bruxelles

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Christiane Gueuning

Université libre de Bruxelles

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E. C. Van Bogaert

Université libre de Bruxelles

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J. Ameryckx

Université libre de Bruxelles

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Anna Popowski

Université libre de Bruxelles

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Claude May-Cocriamont

Université libre de Bruxelles

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Henri Kleiner

Université libre de Bruxelles

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Michèle Brouet-Yager

Université libre de Bruxelles

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Roger Mosselmans

Université libre de Bruxelles

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