C.I. Robison

Effect of rearing environment on bone growth of pullets

Alternative housing systems for laying hens provide mechanical loading and help reduce bone loss. Moreover, achieving greater peak bone mass during pullet phase can be crucial to prevent fractures in the production period. The aim of this study was to determine the housing system effects on bone quality of pullets. Tibiae and humeri of White Leghorn pullets reared in conventional cages (CCs) and a cage-free aviary (AV) system were studied. At 16 wk, 120 birds at random from each housing system were euthanized. Right and left tibiae and humeri were collected and further analyzed. Cortical bone density and thickness were measured using computed tomography. Periosteal and endosteal dimensions were measured at the fracture site during mechanical testing. At 4, 8, 12, and 16 wk, serum concentrations of osteocalcin and hydroxylysyl pyridinoline were analyzed as markers of bone formation and resorption. Cortical bone density was higher (P < 0.05) in humeri of AV pullets, and tibiae were denser (P < 0.05) for AV pullets in the distal section of the bone compared to CC pullets. Ash content was higher (P < 0.05) in AV humeri with no difference in tibiae ash content. Tibiae and humeri of AV pullets had a thicker cortex than the CC pullets (P < 0.05). Additionally, the tibiae and humeri of AV pullets had greater (P < 0.05) second moment of areas than the CC pullets. While some bone material properties between groups were different (P < 0.05), the differences were so small (< 7%) that they likely have no clinical significance. Serum osteocalcin concentrations were not different between the treatments, but hydroxylsyl pyridinoline concentrations were higher in CC pullets at 12 wk compared to the AV pullets and the effect reversed at 16 wk (P < 0.05). These findings indicate that tibiae and humeri respond differently to load bearing activities during growth. The improved load bearing capability and stiffness in bones of AV pullets were related to increased cross-sectional geometry.

Microbiological impact of three commercial laying hen housing systems

Hen housing for commercial egg production continues to be a societal and regulatory concern. Controlled studies have examined various aspects of egg safety, but a comprehensive assessment of commercial hen housing systems in the US has not been conducted. The current study is part of a holistic, multidisciplinary comparison of the diverse aspects of commercial conventional cage, enriched colony cage, and cage-free aviary housing systems and focuses on environmental and egg microbiology. Environmental swabs and eggshell pools were collected from all housing systems during 4 production periods. Total aerobes and coliforms were enumerated, and the prevalence of Salmonella and Campylobacter spp. was determined. Environmental aerobic and coliform counts were highest for aviary drag swabs (7.5 and 4.0 log cfu/mL, respectively) and enriched colony cage scratch pad swabs (6.8 and 3.8 log cfu/mL, respectively). Aviary floor and system wire shell pools had the greatest levels of aerobic contamination for all eggshell pools (4.9 and 4.1 log cfu/mL, respectively). Hens from all housing systems were shedding Salmonella spp. (89–100% of manure belt scraper blade swabs). The dry belt litter removal processes for all housing systems appear to affect Campylobacter spp. detection (0–41% of manure belt scraper blade swabs) considering detection of Campylobacter spp. was much higher for other environmental samples. Aviary forage area drag swabs were 100% contaminated with Campylobacter spp., whereas enriched colony cage scratch pads had a 93% positive rate. There were no differences in pathogen detection in the shell pools from the 3 housing systems. Results indicate egg safety is enhanced when hens in alternative housing systems use nest boxes. Additionally, current outcomes indicate the use of scratch pads in hen housing systems needs to be more thoroughly investigated for effects on hen health and egg safety.

Influence of commercial laying hen housing systems on the incidence and identification of Salmonella and Campylobacter

The housing of laying hens is important for social, industrial, and regulatory aspects. Many studies have compared hen housing systems on the research farm, but few have fully examined commercial housing systems and management strategies. The current study compared hens housed in commercial cage-free aviary, conventional cage, and enriched colony cage systems. Environmental and eggshell pool samples were collected from selected cages/segments of the housing systems throughout the production cycle and monitored for Salmonella and Campylobacter prevalence. At 77 wk of age, 120 hens per housing system were examined for Salmonella and Campylobacter colonization in the: adrenal glands, spleen, ceca, follicles, and upper reproductive tract. All isolates detected from environmental swabs, eggshell pools, and tissues were identified for serotype. Two predominant Salmonella were detected in all samples: S. Braenderup and S. Kentucky. Campylobacter coli and C. jejuni were the only Campylobacter detected in the flocks. Across all housing systems, approximately 7% of hens were colonized with Salmonella, whereas > 90% were colonized with Campylobacter. Salmonella Braenderup was the isolate most frequently detected in environmental swabs (P < 0.0001) and housing system impacted Salmonella spp. shedding (P < 0.0001). Campylobacter jejuni was the isolate most frequently found in environmental swabs (P < 0.01), while housing system impacted the prevalence of C. coli and jejuni in ceca (P < 0.0001). The results of this study provide a greater understanding of the impact of hen housing systems on hen health and product safety. Additionally, producers and academia can utilize the findings to make informed decisions on hen housing and management strategies to enhance hen health and food safety.

Duck gait: Relationship to hip angle, bone ash, bone density, and morphology

The rapid growth meat birds, including ducks, undergo requires skeletal integrity; however, fast growth may not be conducive to adequate bone structure. A relationship likely exists between skeletal changes and duck mobility. Reduced mobility in meat ducks may have impacts on welfare and production. This study examined the relationships among gait score, bone parameters, and hip angle. Commercial Pekin ducks, ages 14 d (n = 100), 21 d (n = 100), and 32 d (n = 100) were weighed and gait scored with a 3-point gait score system by an observer as they walked over a Tekscan gait analysis system. Gait was scored as GS0, GS1, or GS2 with a score of GS0 defined as good walking ability and a score of GS2 as poorest walking ability. Ducks were humanely euthanized, full body scanned using quantitative computed tomography (QCT), and the right femur and tibia were extracted. Leg bones were cleaned, measured, fat extracted, and ashed. QCT scans were rendered to create computerized 3D models where pelvic hip angles and bone density were measured. Statistical analysis was conducted using PROC MIXED with age and gait score in the model. Body weight increased with age, but within an age, body weight decreased as walking ability became worse (P < 0.01). As expected, linear increases in tibia and femur bone width and length were observed as the ducks aged (P < 0.01). Right and left hip angle increased with duck age (P < 0.01). Additionally, ducks with a GS2 had wider hip angles opposed to ducks with a GS0 (P < 0.01). Bone density increased linearly with both age and gait score (P < 0.05). Femur ash content was lowest in 32-day-old ducks and ducks with GS1 and GS2 (P < 0.0001). Tibia ash content increased with age, but decreased as gait score increased (P < 0.001). The observation that right hip angle changed with gait scores merits further investigation into the relationship between duck mobility and skeletal changes during growth.

Keel bone differences in laying hens housed in enriched colony cages

ABSTRACT Keel bone damage may be painful to birds and affect their production. In order to better understand the frequency, position, and timepoint of keel bone damage that occur during production, the integrity of W‐36 laying hen keel bones housed in enriched colony cages at 748.4 cm2 (116 in2) was evaluated. At four time points, 120 birds (10 per cage; three cages per each of four rooms) had keel bones evaluated. Each hen was placed in a motion limiting restraint, scanned using computed tomography (CT), fitted in vests containing tri‐axial accelerometers, and placed back in their cages for 21 d. After 21 d, the hens were rescanned and returned to their cages. This process was repeated after 133 d. The CT scans were imported into Mimics analysis software (Materialise, Plymouth, MI, USA); 3D models were made of each keel bone at each time point and exported to 3‐matic analysis software (Materialise, Plymouth, MI, USA). Each laying hens keel bone model was superimposed onto scans from multiple time points resulting in four bone pairings representative of each 21‐d period, the 133‐d period, and the entire duration of the project. Next, the proximal portion of each bone pairing was edited to normalize bone shape according to a strict protocol. Additionally, each pairing was divided into three portions: distal aspect (3 cm), proximal aspect (2 cm), and middle portion (remaining). Whole bone pairing and each bone portion was analyzed using the Part Comparison tool in 3‐matic. Raw data were compiled into three datasets and analyzed in SAS 9.3 using the GLIMMIX procedure using a three‐level random intercept model. The model controlled for time, part, part(time), and system with random intercepts of bird(cage) and cage. Overall, results revealed that the greatest morphological changes occurred during the first 21‐d period with regards to time (P = 0.03) and in the distal aspect of the keel with regards to part (P < 0.0001).

Chopping Hay Before Feeding Does Not Influence Fecal Particle Size, Blood Variables, or Water Intake in 3-Year-Old Arabians

Abstract Previous work suggested that feeding chopped roughage, rather than long‐stem, may influence thermoregulation during prolonged exercise, potentially by reducing hindgut particle size, thereby influencing water dynamics. This study aimed to determine if chopping hay affects fecal particle size (FPS), packed cell volume (PCV), plasma total solids (TS), and fecal dry matter (FDM). Six 3‐year‐old Arabians were divided into two groups and initially, for acclimation, fed either long‐stem alfalfa hay or the same hay chopped (3–6 cm length) for a week each in a cross‐over design; in weeks 3 and 4, this cross‐over repeated. On days 6 and 7 of weeks 3 and 4, water consumption was measured over 48 hours, feed intake time was recorded, and PCV plus TS were measured at 0, 30, 60, 90, 120, 150, and 180 minutes after feeding. Fecal samples were collected each morning during weeks 3 and 4 to determine FDM and FPS. Packed cell volume and TS increased over the 3‐hour sampling period (P < .01), but chopping the hay had no effect on the hay intake time, PCV, or TS. Water consumption and FDM did not differ between treatments (P = .9; P = .24) and there was no effect of treatment on FPS (P = .50). Only 10% of the fecal particles did not pass through the largest 2.36 mm sieve and 44% passed through the 150 &mgr;m sieve, suggesting marked reduction in particle size during the digestive process regardless of original hay intake length. Chopping hay did not have an impact on measured variables including FPS. HighlightsReducing hay particle size has no influence on total tract particle size reduction.Chopping hay did not alter fecal dry matter, water intake, or consumption time.Chopping hay did not influence packed cell volume or plasma total solids.Chopping hay did not influence hydration status.

Effects of different litter substrates and induced molt on production performance and welfare quality parameters of white Leghorn hens housed in multi-tiered aviary system

More than 90% of the commercial egg production in the United States is pledged to be in cage-free systems by 2025. Management practices like induced molting and litter area management have come under scrutiny because of the housing system change. The aim of this study was to determine the welfare and production implications of different litter substrates and also evaluate induced molting of hens in a cage-free system. Bovan White hens were housed in a multi-tier aviary system with daily access to open litter area of either Astroturf (AT), wood shavings (SH), or straw (ST) and bare concrete floor (CO) serving as control. At 68 wk of age, molt was induced in half of the hens whereas the other half continued without molting to 116 wk. Production and welfare parameters were measured periodically throughout first and second cycles. Litter substrate did not influence hen-day production and case-weight measurements. However, CO had the lowest total number of eggs produced during the first cycle (P < 0.05). Hen-day percentage was approximately 14% greater in molted hens during the second cycle with egg case weight being heavier in non-molt hens toward the end of second cycle (P < 0.05). The only welfare parameter influenced by litter substrate during the first cycle was a greater crop feather loss in AT than ST at mid-lay (P < 0.05). Keel deformations increased with age irrespective of the litter substrate with 91.5% of palpated hens having keel deformations at the end of first cycle (P < 0.05). Molting did not influence the keel palpation and footpad scores whereas frequency of moderate comb wound was greater in molt hens during molt (P < 0.05). Severe feather loss was seen in non-molt hens during the second cycle (P < 0.05). Litter substrate does not affect production and physical parameters of welfare of hens in a multi-tier aviary system. Additionally, induced molting can be successfully carried out in the multi-tier cage-free system.

Keel bone damage assessment: consistency in enriched colony laying hens

ABSTRACT Damage to the keel bone is a major issue in the laying hen industry. The goal of this study was to compare palpation results of live laying hens to digital computed tomography (CT) images, to assess changes in palpation reliability as training and familiarity increased, and to examine keel bone morphology over time. The longitudinal study consisted of 2 trials of 3 observation periods using 40 different (n = 120) W‐36 hens housed in enriched colony cages. The first trial began when hens were 52 to 58 wk of age repeating the trial when the same birds were 74 to 81 wk of age. At 52 wk of age, each hens keel bone was palpated by a single individual for keel bone caudal tip fractures (Tip), sagittal deviations (Evenness), and transverse deviations (Straightness). After palpation, each hen was placed in a motion limiting restraint and scanned using CT. The hens spent the next 21 d in their cages and on day 21, the hens were collected, palpated, and CT scanned again. The CT scans were imported into Mimics analysis software, 3D models of each keel bone were constructed and evaluated. Each bone and 3D model was scored (0, 1, 2) on the measurement of transverse deviation based on <0.5 cm, 0.51 to 1.0 cm, and >1.0 cm total deviation, respectively. Analysis of data using Proc Freq and Means in SAS 9.3 revealed minimal to moderate kappa values and moderate agreement percentages between palpators and digital analysis. The computer generated 3D models of individual keel bones were compared to palpation scores for Tip, Evenness, and Straightness at the beginning and end of each trial. The visual observations of the 3D models were qualitative, performed by a single individual. Overall, we found CT scanning to be a useful tool in observing changes to the keel bone, we observed changes in palpation accuracy as training/familiarity increased, and examined changes in keel morphology, specifically in the tip, after 52 wk of age.

Correlation analysis of cortical geometry of tibia and humerus of white leghorns using clinical quantitative computed tomography and microcomputed tomography scans1

&NA; Peripheral quantitative computed tomography (QCT) has been used in poultry bone research in recent years to analyze cortical and cross‐sectional geometry. For QCT to be used as a standard research tool for analysis of bones of laying hens (cortical thickness <2 mm), the accuracy of the scans must be assessed. The primary difference between the QCT and micro‐computed tomography (micro‐CT) is image resolution. Image resolution is inversely related to the pixel size. The aim of the current study was to correlate the cortical parameters measured using clinical CT scans with the measurements from micro‐CT, the current gold standard. A total of 15 tibiae and 14 humeri of Lohmann White hens was scanned using clinical CT and micro‐CT. Reconstruction of the scans generated images with final voxel resolution of 195 &mgr;m for clinical CT scans and 46 &mgr;m for micro‐CT scans. Cortical and total area were measured using MIMICS® software at proximal, middle, and distal locations of 20 mm sections of humerus diaphysis and 30 mm sections of tibia diaphysis. The total area for proximal and middle locations as well as proximal cortical area measurements for humeri produced strong correlation coefficients (R ≥ 0.70). Moderate strength correlation coefficients (R = 0.40 to 0.60) in humeri were seen in middle and distal cortical areas. Distal total area in humeri displayed a weak correlation coefficient (R ≤ 0.3; P = 0.25). Overall, tibiae demonstrated a weaker correlation. Proximal and middle cortical areas indicated moderate correlation coefficients (R = 0.40 to 0.60), while proximal and middle total areas accompanied by distal cortical and total area displayed weak correlation coefficients (R ≤ 0.3). Only the middle cortical area measurement for tibiae was significant (P = 0.03). These results indicate stronger correlation for humeri measurements among the scans than tibia. Overall, cross‐sectional area measurements were only low to moderately correlated between clinical and micro‐CT scans.

Acute and Prolonged Effects of Vibrating Platform Treatment on Horses: A Pilot Study

ABSTRACT Anecdotal reports suggest using vibrating platforms may improve performance and promote healing in competitive horses. This study tested the hypothesis that physiological parameters are altered with vibration therapy. Detailed musculoskeletal examinations were performed on six Arabian horses (average age = 22 years) by two equine veterinarians specializing in sports medicine to score any lameness (before and after limb flexion) or limited range of joint motion. Stride lengths were also determined at the walk and trot. Age, gender, lameness scores, and stride lengths were used to stratify and pair‐match horses on one of two treatments – vibration therapy (VT) or sham control (CO). Horses were re‐evaluated (by the same blinded veterinarian) acutely, after one initial 30‐minute treatment, and again after 3 weeks, with treatments repeated daily (5 days per week). VT horses stood on a platform vibrating at 50 Hz for 30 minutes, and CO horses stood on an adjacent platform that was not turned on for 30 minutes. Data were analyzed using PROC MIXED in SAS with horse, treatment, test, and day differences. Findings suggest no differences from pre‐ to post‐treatment between VT and CO groups in any parameters measured (P > .05). Qualitative behavior observations suggested that all VT horses stood calmly and appeared more relaxed throughout the treatment period, while CO horses were comparatively restless. It could be questioned whether this observed change warrants VT treatment given few other measured differences but it could explain the growing belief in the horse industry that the treatment is beneficial. HighlightsHorses receiving vibration therapy (VT) did not experience improvements in lameness.While undergoing vibration treatment, horses appeared to elicit signs of relaxation.Evidence did not support VT superseding current lameness treatments.