C.M. Kao

Regulating colored textile wastewater by 3/31 wavelength ADMI methods in Taiwan.

The wastewater from textile dyeing facilities is difficult to treat satisfactorily because of high compositional variability and high color intensity. To reduce colored effluents discharged into watercourses, the government of Taiwan adopted the Effluent True Color Standard in 1998. The true color discharge limit is 400 American Dye Manufactures Institute (ADMI) units. The adopted analytical method is the ADMI Tristimulus Filter Method (3 wavelength (WL) method), and the 31 WL ADMI method might be also adopted as an alternative for color value measurement. The refractory nature of textile dyes and the introduction of this new regulation present an environmental challenge to the Taiwanese textile industry. The main objectives of this study were to (1) evaluate the efficacy of current wastewater treatment systems for controlling the colored textile wastewater discharges, and (2) evaluate the correlations between 3 and 31 WL ADMI methods. Ten representative textile wastewater treatment facilities employing biological and chemical coagulation treatment technologies were selected to perform a 10-consecutive-day effluent sampling and analysis. Results show that a significant difference between 3 and 31 ADMI methods was observed. These two ADMI methods cannot be substituted for each other, and the discharge standard should be determined based on the selected testing method. Investigation results also suggest that the commonly used wastewater treatment technology (biological + chemical coagulation) fails to effectively remove dye from the colored textile wastewater. Sodium hypochlorite (NaOCl) addition was applied by most facilities as the temporary post-polishment step to comply with the color discharge standard.

Enhanced TCDD degradation by Fenton's reagent preoxidation.

The dioxin isomer 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been called the most toxic compound known to man. Because of its poor bioavailability and low biodegradibility, bioremediation technology cannot effectively degrade TCDD when used alone. In this study, chemical pretreatment (partial oxidation) in combination with biodegradation technique was developed to efficiently remediate TCDD-contaminated soils. An oxidizing reagent [Fentons Reagent (FR)] was applied in a slurry reactor to transform TCDD with a concentration of 96 microg per kg of soil to compounds more amenable to biodegradation. Up to 99% TCDD was transformed after the chemical pretreatment process. The slurry reactor was then converted to a bioreactor for the following biodegradation experiment. The detected TCDD oxidation byproducts including chlorophenols (CPs) and chlorobenzenes (CBs) were transformed in this bioreactor under aerobic conditions. Two other biodegradation experiments were performed in parallel to investigate the biodegradabiliy of TCDD under aerobic and anaerobic conditions without chemical pretreatment. Approximately 53% TCDD was transformed under anaerobic conditions possibly due to the reductive dechlorination process using organic materials contained in the activated sludge as the primary substrates. No TCDD degradation was observed under aerobic conditions. Results show that FR can oxidize TCDD to less-chlorinated and less-toxic byproducts, promoting their bioavailability to microbial communities. The bench-scale results indicate that the two-stage (partial oxidation followed by biodegradation) system has the potential to be developed to remediate TCDD-contaminated soils on-site.

Intrinsic bioremediation of trichloroethylene and chlorobenzene: field and laboratory studies

Activities at a former fire training area at Robins Air Force Base in Georgia, USA resulted in contamination of groundwater with a mixture of trichloroethylene (TCE) and chlorobenzene (CB). Results from the field investigation suggest that intrinsic bioremediation process is occurring, which caused the decrease in TCE and CB concentrations, and increase in TCE degradation byproducts [e.g., dichloroethylene isomers (DCEs), vinyl chloride (VC)] concentrations. Contaminated groundwater samples collected from this site were used to conduct microbial enumeration tests, and used as the inocula for microcosm establishment. Results from the microbial enumeration study indicate that methanogenesis was the dominant biodegradation pattern within the source and mid-plume areas, and the aerobic biodegradation process dominated the downgradient area. Laboratory microcosm experiments were conducted to evaluate the feasibility of using CB as the primary substrate to enhance the intrinsic biodegradation of TCE. Microcosm results suggest that CB can serve as the primary substrate (electron donor), and enhance TCE biodegradation to less-chlorinated compounds under both aerobic cometabolism and reductive dechlorination conditions.

Application of surfactant enhanced permanganate oxidation and bidegradation of trichloroethylene in groundwater.

The industrial solvent trichloroethylene (TCE) is among the most ubiquitous chlorinated solvents found in groundwater contamination. The main objectives of this study were to evaluate the feasibility of using non-ionic surfactant Simple Green (SG) to enhance the oxidative dechlorination of TCE by potassium permanganate (KMnO4) employing a continuous stir batch reactor system (CSBR) and column experiments. The effect of using surfactant SG to enhance the biodegradation of TCE via aerobic cometabolism was also examined. Results from CSBR experiments revealed that combination of KMnO4 with surfactant SG significantly enhanced contaminant removal, particularly when the surfactant SG concentrated at its CMC. TCE degradation rates ranged from 74.1% to 85.7% without addition of surfactant SG while TCE degradation rates increased to ranging from 83.8% to 96.3% with presence of 0.1wt% SG. Furthermore, results from column experiments showed that TCE was degraded from 38.1microM to 6.2microM in equivalent to 83.7% of TCE oxidation during first 560min reaction. This study has also demonstrated that the addition of surfactant SG is a feasible method to enhance bioremediation efficiency for TCE contaminated groundwater. The complete TCE degradation was detected after 75 days of incubation with both 0.01 and 0.1wt% of surfactant SG addition. Results revealed that surfactant enhanced chemical oxidation and bioremediation technology is one of feasible approaches to clean up TCE contaminated groundwater.

Application of persulfate-releasing barrier to remediate MTBE and benzene contaminated groundwater.

The objective of this study was to assess the potential of using an in situ oxidation barrier system to remediate gasoline-contaminated groundwater. The passive remedial system included a persulfate-releasing barrier containing persulfate-releasing materials to release persulfate for contaminant oxidation. Bench experiments were performed to determine the components and persulfate-releasing rate of the persulfate-releasing materials. Column experiments were conducted to evaluate the effectiveness of the designed persulfate-releasing materials on the control of petroleum-hydrocarbon plume. In this study, methyl tert-butyl ether (MTBE) and benzene were used as the target compounds. The optimal persulfate releasing rate was obtained when the mass ratio of persulfate/cement/sand/water was 1/1/0.16/0.5, and the rate varied from 31 to 8 mg persulfate per day per g of material. Significant amounts of MTBE and benzene were removed through the oxidation process due to the release of persulfate, and the produced tert-butyl formate (TBF) and tert-butyl alcohol (TBA), byproducts of MTBE, were further oxidized in the system. Results suggest that the oxidation rate would be affected by the oxidant reduction potential and concentrations of ferrous iron and persulfate.

Treatment of tetrachloroethylene-contaminated groundwater by surfactant-enhanced persulfate/BOF slag oxidation--a laboratory feasibility study.

The main objective of this study was to evaluate the feasibility of remediating tetrachloroethylene (PCE)-contaminated groundwater (with initial PCE concentration of approximately 20 mg L(-1)) via persulfate oxidation activated by basic oxygen furnace slag (S(2)O(8)(2-)/BOF slag) with the addition of biodegradable surfactant (Tween 80). Results indicate that only 15% of PCE can be removed in experiment with the addition of S(2)O(8)(2-) only (S(2)O(8)(2-)/PCE=30/1). PCE removal can be increased to 31% while both S(2)O(8)(2-) and BOF slag (10 g L(-1)) were added. This indicates that BOF slag was able to activate the persulfate oxidation mechanism, and cause the decrease in PCE concentration via oxidation process. Results also reveal that PCE degradation rates increased to 92% with the presence of Tween 80 (S(2)O(8)(2-)/Tween 80/PCE=30/2/1). In the presence of 10 g L(-1) BOF slag, the reaction rate constant (k(obs)) values were found to be 3.1 x 10(-3), 8.7 x 10(-3), 1.6 x 10(-2), and 5.8 x 10(-2)h(-1), as the S(2)O(8)(2-)/Tween 80/PCE molar ratios were 30/0/1, 30/0.5/1, 30/1/1, and 30/2/1, respectively. The reaction rate constant increased as the Tween 80 concentration increased. The significantly increased k(obs) could be caused by the enhanced solubilization of PCE by Tween 80. The increase in initial surfactant concentration would cause the increase in the solubilization of PCE, and thus, enhance the oxidation rate. This was confirmed by the total amount of chloride ions produced after the reaction. Results from this study indicate that BOF slag-activated persulfate oxidation enhanced by surfactant addition is a potential method to efficiently and effectively remediate chlorinated solvents contaminated groundwater.

Development of a slow polycolloid-releasing substrate (SPRS) biobarrier to remediate TCE-contaminated aquifers.

In this study, an in situ slow polycolloid-releasing substrate (SPRS) biobarrier system was developed to continuously provide biodegradable substrates for the enhancement of trichloroethylene (TCE) reductive dechlorination. The produced SPRS contained vegetable oil (used as a slow-released substrate), cane molasses [used as an early-stage (fast-degradable) substrate], and surfactants [Simple Green (SG) and soya lecithin (SL)]. An emulsification study was performed to evaluate the globule droplet size and stability of SPRS. The distribution and migration of the SPRS were evaluated in a column experiment, and an anaerobic microcosm study was performed to assess the capability of SPRS to serve as a slow and long-term carbon-releasing substrate for TCE dechlorination. The results show that a stable oil-in-water (W/O, 50/50) emulsion (SPRS) with uniformly small droplets (D₁₀, 0.93 μm) has been produced, continuously supplying primary substrates. The emulsion containing the surfactant mixture (with 72 mg/L SL and 71 mg/L SG) had a small absolute value of the zeta potential, which reduced the inter-particle repulsion, leading the emulsion droplets to adhere to one another after collision. The addition of SPRS creates anaerobic conditions and leads to a more complete and thorough removal of TCE through biodegradation and sorption mechanisms.

Remediation of TCE-contaminated groundwater using acid/BOF slag enhanced chemical oxidation.

The objective of this study was to evaluate the potential of applying acid/H(2)O(2)/basic oxygen furnace slag (BOF slag) and acid/S(2)O(8)(2-)/BOF slag systems to enhance the chemical oxidation of trichloroethylene (TCE)-contaminated groundwater. Results from the bench-scale study indicate that TCE oxidation via the Fenton-like oxidation process can be enhanced with the addition of BOF slag at low pH (pH=2-5.2) and neutral (pH=7.1) conditions. Because the BOF slag has iron abundant properties (14% of FeO and 6% of Fe(2)O(3)), it can be sustainably reused for the supplement of iron minerals during the Fenton-like or persulfate oxidation processes. Results indicate that higher TCE removal efficiency (84%) was obtained with the addition of inorganic acid for the activation of Fenton-like reaction compared with the experiments with organic acids addition (with efficiency of 10-15% lower) (BOF slag=10gL(-1); initial pH=5.2). This could be due to the fact that organic acids would compete with TCE for available oxidants. Results also indicate that the pH value had a linear correlation with the observed first-order decay constant of TCE, and thus, lower pH caused a higher TCE oxidation rate.

Application of polycolloid-releasing substrate to remediate trichloroethylene-contaminated groundwater: a pilot-scale study.

The objectives of this pilot-scale study were to (1) evaluate the effectiveness of bioremediation of trichloroethylene (TCE)-contaminated groundwater with the supplement of slow polycolloid-releasing substrate (SPRS) (contained vegetable oil, cane molasses, surfactants) under reductive dechlorinating conditions, (2) apply gene analyses to confirm the existence of TCE-dechlorinating genes, and (3) apply the real-time polymerase chain reaction (PCR) to evaluate the variations in TCE-dechlorinating bacteria (Dehalococcoides spp.). Approximately 350L of SPRS solution was supplied into an injection well (IW) and groundwater samples were collected and analyzed from IW and monitor wells periodically. Results show that the SPRS caused a rapid increase of the total organic carbon concentration (up to 5794mg/L), and reductive dechlorination of TCE was significantly enhanced. TCE dechlorination byproducts were observed and up to 99% of TCE removal (initial TCE concentration=1872μg/L) was observed after 50 days of operation. The population of Dehalococcoides spp. increased from 4.6×10(1) to 3.41×10(7)cells/L after 20 days of operation. DNA sequencing results show that there were 31 bacterial species verified, which might be related to TCE biodegradation. Results demonstrate that the microbial analysis and real-time PCR are useful tools to evaluate the effectiveness of TCE reductive dechlorination.

Crude aqueous extracts of Pluchea indica (L.) Less. inhibit proliferation and migration of cancer cells through induction of p53-dependent cell death

BackgroundPluchea indica (L.) Less. (Asteraceae) is a perennial shrub plant with anti-inflammatory and antioxidant medicinal properties. However, the anti-cancer properties of its aqueous extracts have not been studied. The aim of this study was to investigate the anti-proliferation, anti-migration, and pro-apoptotic properties of crude aqueous extracts of P. indica leaf and root on human malignant glioma cancer cells and human cervical cancer cells, and the underlying molecular mechanism.MethodsGBM8401 human glioma cells and HeLa cervical carcinoma cells were treated with various concentrations of crude aqueous extracts of P. indica leaf and root and cancer cell proliferation and viability were measured by cell growth curves, trypan blue exclusions, and the tetrazolium reduction assay. Effects of the crude aqueous extracts on focus formation, migration, and apoptosis of cancer cells were studied as well. The molecular mechanism that contributed to the anti-cancer activities of crude aqueous extracts of P. indica root was also examined using Western blotting analysis.ResultsCrude aqueous extracts of P. indica leaf and root suppressed proliferation, viability, and migration of GBM8401 and HeLa cells. Treatment with crude aqueous extracts of P. indica leaf and root for 48 hours resulted in a significant 75% and 70% inhibition on proliferation and viability of GBM8401 and HeLa cancer cells, respectively. Crude aqueous extracts of P. indica root inhibited focus formation and promoted apoptosis of HeLa cells. It was found that phosphorylated-p53 and p21 were induced in GBM8401 and HeLa cells treated with crude aqueous extracts of P. indica root. Expression of phosphorylated-AKT was decreased in HeLa cells treated with crude aqueous extracts of P. indica root.ConclusionThe in vitro anti-cancer effects of crude aqueous extracts of P. indica leaf and root indicate that it has sufficient potential to warrant further examination and development as a new anti-cancer agent.