Caiwen Ou
Southern Medical University
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Publication
Featured researches published by Caiwen Ou.
RSC Advances | 2016
Anqing Huang; Caiwen Ou; Yanbin Cai; Zhongyan Wang; Hekai Li; Zhimou Yang; Minsheng Chen
We report a strategy of in situ forming supramolecular nanofibers of taxol-phosphorylated peptide conjugates catalyzed by phosphatase for efficiently killing cancer cells.
Journal of Cardiovascular Pharmacology | 2017
Zhiye Wu; Anqing Huang; Jianyun Yan; Bei Liu; Qicai Liu; Jianwu Zhang; Xiuli Zhang; Caiwen Ou; Minsheng Chen
Abstract: The aim of this study was to explore the effect and mechanism of action of resveratrol (RSV) on cardiac function in diabetic cardiomyopathy (DCM). Hyperglycemia-induced apoptosis contributes to the pathogenic changes in DCM. RSV treatment inhibited high glucose–induced apoptosis of neonatal rat ventricular myocytes. Additionally, high glucose decreased cell viability, prevented serine–threonine kinase (Akt) and FoxO3a phosphorylation, and suppressed cytoplasmic translocation of FoxO3a. However, these effects of apoptosis were reversed by 10 &mgr;M of RSV. The PI3K inhibitor LY294002 abolished the RSV protective effect in vitro. RSV (5 or 50 mg·kg−1·d−1 orally for 8 weeks) prevented the deterioration of cardiac function and structural cardiomyopathy in a streptozotocin-induced rat model of diabetes and reduced apoptosis in diabetic myocardium. Furthermore, it restored streptozotocin-impaired phosphorylation of Akt and FoxO3a (p-Akt and p-FoxO3a) and suppressed nuclear translocation of FoxO3a in vivo. Together, these data indicate that RSV has therapeutic potential against DCM by inhibiting apoptosis via the PI3K/Akt/FoxO3a pathway.
Cellular Physiology and Biochemistry | 2018
Min Yi; Hekai Li; Zhiye Wu; Jianyun Yan; Qicai Liu; Caiwen Ou; Minsheng Chen
Human neuropeptide Y (hNPY) is one of the most widely expressed neurotransmitters in the human central and peripheral nervous systems. It consists of 36 highly conserved amino acid residues, and was first isolated from the porcine hypothalamus in 1982. While it is the most recently discovered member of the pancreatic polypeptide family (which includes neuropeptide Y, gut-derived hormone peptide YY, and pancreatic polypeptide), NPY is the most abundant peptide found in the mammalian brain. In order to exert particular functions, NPY needs to bind to the NPY receptor to activate specific signaling pathways. NPY receptors belong to the class A or rhodopsin-like G-protein coupled receptor (GPCR) family and signal via cell-surface receptors. By binding to GPCRs, NPY plays a crucial role in various biological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. Abnormal regulation of NPY is involved in the development of a wide range of diseases, including obesity, hypertension, atherosclerosis, epilepsy, metabolic disorders, and many cancers. Thus far, five receptors have been cloned from mammals (Y1, Y2, Y4, Y5, and y6), but only four of these (hY1, hY2, hY4, and hY5) are functional in humans. In this review, we summarize the structural characteristics of human NPY receptors and their role in metabolic diseases.
Scientific Reports | 2016
Chao Chen; Yongquan Hua; Yawen Hu; Yuan Fang; Shenglu Ji; Zhimou Yang; Caiwen Ou; Deling Kong; Dan Ding
As lysosomal protein transmembrane 4 beta (LAPTM4B) is an important biomarker for many solid tumours, development of small-molecule fluorescence light-up probes for detection and imaging of LAPTM4B proteins is particularly valuable. In this work, we reported the design and synthesis of a far-red/near-infrared (FR/NIR) fluorescence light-up probe DBT-2EEGIHGHHIISVG, which could specifically visualize LAPTM4B proteins in cancer cells and tumour-bearing live mice. DBT-2EEGIHGHHIISVG was synthesized by the conjugation of two LAPTM4B-binding peptide ligands (EEGIHGHHIISVG) with one environment-sensitive fluorogen, 4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole (DBT). Owing to the intramolecular charge transfer character of DBT, DBT-2EEGIHGHHIISVG is weakly emissive in aqueous solution, but switches to fluoresce upon LAPTM4B proteins specifically bind to the peptide ligand of the probe, which provide the DBT with hydrophobic microenvironment, greatly reducing its charge transfer effect with water. It is found that DBT-2EEGIHGHHIISVG can achieve targeted imaging of LAPTM4B proteins in HepG2 cancer cells and visualize LAPTM4B protein-expressed tumour tissues of live mice in a selective and high-contrast manner.
RSC Advances | 2014
Caiwen Ou; Jianwu Zhang; Yang Shi; Zhongyan Wang; Ling Wang; Zhimou Yang; Minsheng Chen
We report on a rationally designed D-amino acid doping peptide that can form hydrogels under neutral conditions and can be applied to form a cell colony of HeLa cells.
RSC Advances | 2015
Guoqin Chen; Jiaxin Chen; Qicai Liu; Caiwen Ou; Jie Gao
We report the phosphatase-triggered formation of a meta-stable peptide-based supramolecular hydrogel that can easily change to a clear solution by mechanical forces. Cells cultured in the hydrogel can therefore be separated by pipetting followed by centrifugation.
Scientific Reports | 2017
Zhiye Wu; Guoqin Chen; Jianwu Zhang; Yongquan Hua; Jinliang Li; Bei Liu; Anqing Huang; Hekai Li; Minsheng Chen; Caiwen Ou
The effect of transplanted rat mesenchymal stem cells (MSCs) can be reduced by extracellular microenvironment in myocardial infarction (MI). We tested a novel small-molecular hydrogel (SMH) on whether it could provide a scaffold for hepatocyte growth factor (HGF)-modified MSCs and alleviate ventricular remodeling while preserving cardiac function after MI. Overexpression of HGF in MSCs increased Bcl-2 and reduced Bax and caspase-3 levels in response to hypoxia in vitro. Immunocytochemistry demonstrated that cardiac troponin (cTnT), desmin and connexin 43 expression were significantly enhanced in the 5-azacytidine (5-aza) with SMH group compared with the 5-aza only group in vitro and in vivo. Bioluminescent imaging indicated that retention and survival of transplanted cells was highest when MSCs transfected with adenovirus (ad-HGF) were injected with SMH. Heart function and structure improvement were confirmed by echocardiography and histology in the Ad-HGF-SMHs-MSCs group compared to other groups. Our study showed that: HGF alleviated cell apoptosis and promoted MSC growth. SMHs improved stem cell adhesion, survival and myocardial cell differentiation after MSC transplantation. SMHs combined with modified MSCs significantly decreased the scar area and improved cardiac function.
International Journal of Molecular Medicine | 2017
Xiaobin Ni; Caiwen Ou; Jingbin Guo; Bei Liu; Jianwu Zhang; Zhiye Wu; Hekai Li; Minsheng Chen
Mesenchymal stem cell (MSC) transplantation has emerged as a promising therapy for ischemic heart disease; however, the low survival rate of transplanted cells limits their therapeutic efficacy. The aim of this study was to investigate whether the dual genetic modification of vascular endothelial growth factor (VEGF) and B-cell lymphoma-2 (Bcl-2) confers a higher expression level of the target genes, better survival and a stronger paracrine effect in MSCs in an adverse environment than the modification of the individual genes. For this purpse, a lentiviral vector was constructed by using a self-cleaving T2A peptide sequence to link and achieve the co-overexpression of VEGF and Bcl-2. Rat MSCs were transfected to obtain cell lines that exhibited a stable overexpression. An in vitro model of oxygen glucose deprivation (OGD) was applied to mimic the ischemic microenvironment, and cell apoptosis, autophagy and the paracrine effects were then determined. Compared with the MSCs in which individual genes were modified and the control MSCs, the MSCs which were subjected to dual genetic modification had a higher expression level of the target genes, a more rapid proliferation, reduced apoptosis, decreased autophagy and an enhanced paracrine effect. Furthermore, the suppression of autophagy was found to contribute to the inhibition of apoptosis in this in vitro OGD model. On the whole, these data indicate that the co-overexpression of VEGF and Bcl-2 protects MSCs in an ischemic environment by inhibiting apoptosis, suppressing autophagy and enhancing the paracrine effects.
RSC Advances | 2016
Xiaoli Zhang; Can Li; Youzhi Wang; Caiwen Ou; Shenglu Ji; Minsheng Chen; Zhimou Yang
We report in this paper a molecular hydrogel formed by adding cis-dichlorodiamineplatinum(II) (DDP) to a self-assembling taxol-peptide amphiphile. Our study provides a novel self-assembling nanomedicine and hydrogel to deliver two anti-cancer drugs simultaneously.
RSC Advances | 2014
Guoqin Chen; Jianwu Zhang; Dongxia Li; Chunhua Ren; Caiwen Ou; Ling Wang; Minsheng Chen
We report a selenium containing vancomycin derivative with redox-controllable self-assembly property and anti-bacterial activity.