Camila Fabiano de Freitas
Universidade Estadual de Maringá
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Featured researches published by Camila Fabiano de Freitas.
Photochemistry and Photobiology | 2016
Camila Fabiano de Freitas; Diogo Silva Pellosi; Bianca Martins Estevão; Italo Rodrigo Calori; Tayana Mazin Tsubone; Mario J. Politi; Wilker Caetano; Noboru Hioka
It was evaluated the properties of the xanthene dyes Erythrosin B, Eosin Y and theirs Methyl, Butyl and Decyl ester derivatives as possible photosensitizers (PS) for photodynamic treatments. The more hydrophobic dyes self‐aggregate in water/ethanol solutions above 70% water (vol/vol) in the mixture. In buffered water, these PS were encapsulated in Pluronic polymeric surfactants of P‐123 and F‐127 by two methodologies: direct addition and the thin‐film solid dispersion methods. The thin‐film solid method provided formulations with higher stabilities besides effective encapsulation of the PS as monomers. Size measurements demonstrated that Pluronic forms self‐assembled micelles with uniform size, which present slightly negative surface potential and a spherical form detected by TEM microscopy. The ester length modulates xanthene localization in the micelle, which is deeper with the increase in the alkyl chain. Moreover, some PS are distributed into two populations: one on the corona micelle interface shell (PEO layer) and the other into the core (PPO region). Although all PS formulations show high singlet oxygen quantum yield, promising results were obtained for Erythrosin B esters with the hydrophobic P‐123, which ensures their potential as drug for clinical photodynamic applications.
Química Nova | 2014
Paulo Cesar de Souza Pereira; Camila Fabiano de Freitas; Cristiane Santi Chaves; Bianca Martins Estevão; Diogo Silva Pellosi; André L. Tessaro; Vagner Roberto Batistela; Ieda Spacino Scarminio; Wilker Caetano; Noboru Hioka
An experiment was proposed applying the Chemometric approach of Multivariate Analysis for inclusion in undergraduate Chemistry courses to promote and expand the use of this analytical-statistical tool. The experiment entails the determination of the acid dissociation constant of dyes via UV-Vis electronic spectrophotometry. The dyes used show from simple equilibrium to very complex systems involving up to four protolytic species with high spectral overlap. The Chemometric methodology was more efficient than univariate methods. For use in classes, it is up to the teacher to decide which systems should be utilized given the time constraints and laboratory conditions.
Photodiagnosis and Photodynamic Therapy | 2018
Laíse Midori Tokubo; Pedro Luiz Rosalen; Janaina de Cássia Orlandi Sardi; Irlan de Almeida Freires; Mitsue Fujimaki; Josely Emiko Umeda; Patrícia M. Barbosa; Gabriela Ortolan Tecchio; Noboru Hioka; Camila Fabiano de Freitas; Raquel Sano Suga Terada
BACKGROUND Photodynamic therapy (PDT) has demonstrated promising results in the treatment of several clinical pathologies through the photochemical reaction caused by the combination of a photosensitizer and a light source. The objective of this study was to evaluate the antimicrobial effect of the combination of the photosensitizers (PSs) erythrosine/methylene blue activated by a white halogen light device on Streptococcus mutans biofilm. METHODS Two separate experiments were conducted, the first using the PSs at the concentration of 100 μM, and the second 250 μM. The PSs were tested on S. mutans biofilms cultured for 24 h in isolation, in combination, with and without light activation for 2 min fractionated in 4 periods of 30 s. After treatment, biofilms were diluted and plated on BHI medium and incubated for 24 h for colony forming units (CFU) counting. The results (log10) were analyzed with ANOVA followed by Tukey test (p < 0.05). RESULTS The erythrosine/methylene blue combination activated by white halogen light at 100 and 250 μM, and erythrosine at 250 μM, methylene blue at 250 μM presented significantly reduced cell counts (3.2 log10, 5.3 log10, 4.5 log10, 4.3 log10, respectively) when compared to controls (p < 0.05). CONCLUSION PDT with the combination of erythrosine/methylene blue demonstrated better results that the PSs in isolation regardless of the concentration. The use of this combination at the concentration of 250 μM shows promise as an antibacterial treatment for carious lesions and should be further assessed.
Molecules | 2018
Alex Fiori da Silva; Anabela Borges; Camila Fabiano de Freitas; Noboru Hioka; Jane Martha Graton Mikcha; Manuel Simões
The thermal and chemical-based methods applied for microbial control in the food industry are not always environmentally friendly and may change the nutritional and organoleptic characteristics of the final products. Moreover, the efficacy of sanitizing agents may be reduced when microbial cells are enclosed in biofilms. The objective of this study was to investigate the effect of photodynamic inactivation, using two xanthene dyes (rose bengal and erythrosine) as photosensitizing agents and green LED as a light source, against Staphylococcus aureus, Listeria innocua, Enterococcus hirae and Escherichia coli in both planktonic and biofilm states. Both photosensitizing agents were able to control planktonic cells of all bacteria tested. The treatments altered the physicochemical properties of cells surface and also induced potassium leakage, indicating damage of cell membranes. Although higher concentrations of the photosensitizing agents (ranging from 0.01 to 50.0 μmol/L) were needed to be applied, the culturability of biofilm cells was reduced to undetectable levels. This finding was confirmed by the live/dead staining, where propidium iodide-labeled bacteria numbers reached up to 100%. The overall results demonstrated that photoinactivation by rose bengal and erythrosine may be a powerful candidate for the control of planktonic cells and biofilms in the food sector.
Journal of Photochemistry and Photobiology A-chemistry | 2014
Bianca Martins Estevão; Diogo Silva Pellosi; Camila Fabiano de Freitas; Douglas Vanzin; Douglas Santana Franciscato; Wilker Caetano; Noboru Hioka
Dyes and Pigments | 2013
Diogo Silva Pellosi; Bianca Martins Estevão; Camila Fabiano de Freitas; Tayana M. Tsubone; Wilker Caetano; Noboru Hioka
Current Microbiology | 2015
Natália Norika Yassunaka; Camila Fabiano de Freitas; Bruno Ribeiro Rabello; Patrícia Regina dos Santos; Wilker Caetano; Noboru Hioka; Tania Ueda Nakamura; Benício Alves de Abreu Filho; Jane Martha Graton Mikcha
Química Nova | 2018
Ana Claudia Silva; Camila Fabiano de Freitas; André L. Tessaro; Wilker Caetano; Silvana Maria de Oliveira Santin; Noboru Hioka; Graciana Palioto
Journal of lasers in medical sciences | 2017
Taisa Rocha Navasconi; Vanessa Nesi dos Reis; Camila Fabiano de Freitas; Paulo Cesar de Souza Pereira; Wilker Caetano; Noboru Hioka; Maria Valdrinez Campana Lonardoni; Sandra Mara Alessi Aristides; Thaís Gomes Verzignassi Silveira
XII Latin American Congress on Food Microbiology and Hygiene | 2014
Natália Norika Yassunaka; Camila Benedetti Penha; Alex Fiori da Silva; Patrícia Regina dos Santos; Katieli da Silva Souza; Camila Fabiano de Freitas; Noboru Hioka; Tania Ueda Nakamura; Jane Martha Graton Mikcha