Camila Thieime Rosa

Avaliação do exercício físico como fator de analgesia em um modelo experimental de ciatalgia

OBJECTIVE: To evaluate the efficacy of physical exercise (swimming and jumping), with and without overload, working in reducing the pain of rats undergone to an experimental model of sciatica. METHODS: 24 rats were divided into four groups: Placebo (GP), Swimming Group (NG) Swimming 10% Group (GN10) and Leap Group (GS). All groups were submitted to the experimental sciatica model and assessed for pain post-exercise for the Functional Disability Test and the Von Frey filament. RESULTS: In comparison within groups there were significant differences in the moments after injury with the pre-injury, for both assessment instruments. With Von Frey filament was observed a significant difference in group GN10 and GS in the final moments of evaluation. In comparisons between groups were not statistically significant differences obtained with any assessment instrument. CONCLUSION: Treatment with physical exercise was not effective in reducing pain in rats subjected to experimental sciatica model.

A influência da crioterapia na dor e edema induzidos por sinovite experimental

O objetivo deste estudo foi analisar a influencia da crioterapia na dor e edema advindos de sinovite induzida em ratos. Foram utilizados 12 ratos, distribuidos em dois grupos: Controle (GC) - submetido a inducao de sinovite no joelho direito, e nao tratado; e Tratamento (GT) - submetido a sinovite no joelho direito, e tratado com crioterapia. Para induzir a lesao, foi injetado no espaco tibio-femoral formalina 5%. Para avaliacao da dor foi utilizado o teste de incapacidade funcional, que avaliou a dor durante a marcha do animal (tempo de elevacao da pata - TEP); e para quantificar o edema foi utilizado um paquimetro metalico, na regiao da interlinha do joelho. As avaliacoes ocorreram antes da injecao de formalina (AV1), 1 (AV2) e 2 horas (AV3) apos. Apos 10 minutos da lesao, o membro posterior direito foi submerso em agua com gelo, a 5oC por 20 minutos. A avaliacao do TEP mostrou aumento de 194,03% (AV2) e 169,26% (AV3) para GC; e 134,25% (AV2) e 103,13% (AV3) para GT, com relacao a AV1. Na comparacao entre os grupos, em AV3, houve diminuicao significativa para GT. A avaliacao do edema mostrou aumento do diâmetro, para GC de 39,15% (AV2) e 42,39% (AV3); e 27,91% (AV2) e 14,50% (AV3) para GT, tendo como referencia AV1; sendo que apenas GT apresentou diminuicao significativa entre AV2 e AV3. Conclui-se que os efeitos em curto prazo, da crioterapia, foram significativos para reduzir a dor e edema, em ratos submetidos a inducao de sinovite.

Avaliação do papel de opioides endógenos na analgesia do laser de baixa potência, 820 nm, em joelho de ratos Wistar

BACKGROUND AND OBJECTIVES: Pain may result in incapacity when it is associated to structural injury. Low power laser is useful in therapies aiming at decreasing joint pain and at tissue repair, but it is still somewhat controversial. This study aimed at evaluating whether analgesia induced by 820 nm low power laser is affected by the application of an endogenous opioids inhibitor. METHOD: Twenty-four Wistar rats submitted to hyperalgesia were divided into four groups. G1: untreated; G2: treated with 820 nm laser; G3: naloxone injection before injury and untreated; G4: naloxone and treated with 820 nm laser. To induce hyperalgesia, 100 µL of 5% formalin were injected in the right tibiofemoral joint space. Nociception was evaluated by the time for flinching (TFF) in five moments: AV1 (pre-injury), AV2 (15 min/after), AV3 (30 min/after), AV4 (1 hour after) and AV5 (2 hours after). RESULTS: All groups showed significant difference between AV1 and AV2, but only G2 showed no difference between AV1 and AV3. There have been no differences for remaining moments as compared to AV1. CONCLUSION: Low power 820 nm laser analgesia is affected by naloxone.

Uso do exercício resistido antagonizado por naloxone como fator de analgesia em sinovite aguda de joelho de ratos Wistar

Analgesia arising from exercising can occur via release of endogenous opioids in the central nervous system and periphery. However, the literature remains controversial about exercise ways and actions in pain. Thus, the aim of this study was to evaluate whether resistance exercise produces changes on the nociception and suffers interference by applying an opioid inhibitor. 18 rats divided into three groups were used: G1 - hyperalgesia on right knee and untreated; G2 - hyperalgesia and treated with jumps in water; G3 - hyperalgesia with previous injection of naloxone and subsequent jumps. To produce hyperalgesia,100 ul of 5% formalin was injected in the tibiofemoral joint space. Pain was assessed using a digital von Frey filament on the right medial tibiofemoral joint. The evaluation periods were: pre-injury (EV1) after 15 minutes (EV2) and 30 minutes (EV3) and one hour (EV4). The applied exercise was jumping in water and it occurred after EV2. The animal performed 4 sets of 5 jumps, with an interval of 3 minutes and overload of 50% of body weight. In G1, nociceptive increase was observed, with significant decrease and return to initial baseline values in AV4; G2 showed threshold restoration after exercise and return to baseline; G3 reduced thresholds, without restoration or significant increase in them. We concluded that there was analgesia with use of exercise and that it was altered by blocking beta-endorphin.

Avaliação de parâmetros histomorfométricos em sóleos de ratos submetidos à remobilização por salto em meio aquático

INTRODUCTION: The muscular tissue is able to respond stimuli such as immobilization that induces hypotrophy altering muscle performance and are important methods that aim to reverse these deleterious effects in the post-immobilization. OBJECTIVE: The aim of this study was to evaluate transverse and longitudinal histomorphometric parameters of the soleus muscle fibers of rats immobilized in the shortened position and submitted to remobilization by jumping in water. METHODS: It was used 24 rats divided into 3 groups: G1 - remobilized freely, G2 - remobilized with jumps daily, and G3 - jumps on alternate days. The immobilization and remobilization occurred in 2 weeks for the right limb. The variables analyzed were: muscle mass, muscle fiber diameter, length and series sarcomeres estimate along the muscle. RESULTS: There was a reduction in muscle mass for both groups, to the diameter there was difference in G1 and G3, to the muscle length no significant differences, however, for the series sarcomeres estimate was found significant changes in all the groups. CONCLUSION: The protocol used has partial action against the deleterious effects of immobilization.

Avaliação de sóleos de ratas Wistar ooforectomizadas e remobilizadas em meio aquático

INTRODUCCION: a la incertidumbre sobre la manera mas eficaz de removilizacion de los musculos despues de periodos de inmovilizacion.OBJETIVO: Evaluar el comportamiento de removilizacion con natacion sobre parametros histomorfometricos del musculo soleo de ratas ooforectomizadas y pseudo ooforectomizadas.METODOS: Se utilizaron 24 ratas Wistar subdivididas en cuatro grupos: G1: sometidas a ooforectomia, inmovilizacion por 15 dias y removilizadas libremente; G2: ooforectomia, inmovilizacion y removilizadas con natacion por 10 dias; G3: pseudo ooforectomia, inmovilizacion y removilizadas libremente; G4: pseudo ooforectomia, inmovilizacion y removilizadas con natacion. Los musculos soleos derechos e izquierdos fueron disecados para analisis histomorfometricos longitudinal y transversal. El recuento de los sarcomeros se hizo en 300 micras y la longitud de la fibra muscular fue medida por paquimetro analogico. El peso muscular fue obtenido en balanza analitica y el diametro se realizo en 100 fibras por musculo.RESULTADOS: Hubo reduccion significativa tanto en el analisis longitudinal como en el transversal cuando se compararon los musculos soleos derechos e izquierdos de ambos grupos.CONCLUSION: La inmovilizacion del musculo soleo de ratas ooforectomizadas y pseudo ooforectomizadas causa efectos nocivos para la morfometria muscular y la removilizacion por medio de la natacion no fue capaz de promover el retorno de los patrones estructurales del musculo soleo.

Assessment of Wistar rats soleus ovariectomized and remobilized in water

INTRODUCCION: a la incertidumbre sobre la manera mas eficaz de removilizacion de los musculos despues de periodos de inmovilizacion.OBJETIVO: Evaluar el comportamiento de removilizacion con natacion sobre parametros histomorfometricos del musculo soleo de ratas ooforectomizadas y pseudo ooforectomizadas.METODOS: Se utilizaron 24 ratas Wistar subdivididas en cuatro grupos: G1: sometidas a ooforectomia, inmovilizacion por 15 dias y removilizadas libremente; G2: ooforectomia, inmovilizacion y removilizadas con natacion por 10 dias; G3: pseudo ooforectomia, inmovilizacion y removilizadas libremente; G4: pseudo ooforectomia, inmovilizacion y removilizadas con natacion. Los musculos soleos derechos e izquierdos fueron disecados para analisis histomorfometricos longitudinal y transversal. El recuento de los sarcomeros se hizo en 300 micras y la longitud de la fibra muscular fue medida por paquimetro analogico. El peso muscular fue obtenido en balanza analitica y el diametro se realizo en 100 fibras por musculo.RESULTADOS: Hubo reduccion significativa tanto en el analisis longitudinal como en el transversal cuando se compararon los musculos soleos derechos e izquierdos de ambos grupos.CONCLUSION: La inmovilizacion del musculo soleo de ratas ooforectomizadas y pseudo ooforectomizadas causa efectos nocivos para la morfometria muscular y la removilizacion por medio de la natacion no fue capaz de promover el retorno de los patrones estructurales del musculo soleo.

Evaluación de sóleos de ratas Wistar ooforectomizadas y removilizadas en medio acuático

INTRODUCCION: a la incertidumbre sobre la manera mas eficaz de removilizacion de los musculos despues de periodos de inmovilizacion.OBJETIVO: Evaluar el comportamiento de removilizacion con natacion sobre parametros histomorfometricos del musculo soleo de ratas ooforectomizadas y pseudo ooforectomizadas.METODOS: Se utilizaron 24 ratas Wistar subdivididas en cuatro grupos: G1: sometidas a ooforectomia, inmovilizacion por 15 dias y removilizadas libremente; G2: ooforectomia, inmovilizacion y removilizadas con natacion por 10 dias; G3: pseudo ooforectomia, inmovilizacion y removilizadas libremente; G4: pseudo ooforectomia, inmovilizacion y removilizadas con natacion. Los musculos soleos derechos e izquierdos fueron disecados para analisis histomorfometricos longitudinal y transversal. El recuento de los sarcomeros se hizo en 300 micras y la longitud de la fibra muscular fue medida por paquimetro analogico. El peso muscular fue obtenido en balanza analitica y el diametro se realizo en 100 fibras por musculo.RESULTADOS: Hubo reduccion significativa tanto en el analisis longitudinal como en el transversal cuando se compararon los musculos soleos derechos e izquierdos de ambos grupos.CONCLUSION: La inmovilizacion del musculo soleo de ratas ooforectomizadas y pseudo ooforectomizadas causa efectos nocivos para la morfometria muscular y la removilizacion por medio de la natacion no fue capaz de promover el retorno de los patrones estructurales del musculo soleo.