Carina Martins de Moraes

Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil

A brucelose e uma enfermidade infecto-contagiosa que causa grandes perdas economicas a cadeia produtiva da carne e do leite, como consequencia dos disturbios reprodutivos nos animais, alem de ser uma antropozoonose cronica. O objetivo deste estudo foi detectar DNA de Brucella spp. e fazer a distincao da cepa vacinal (B19) da cepa de infeccao de campo. Foram adquiridas 66 amostras de diferentes queijos produzidos e comercializados em tres estados pertencentes a Amazonia brasileira: Amapa (05), Para (55) e Rondonia (06), somando 39 amostras de queijo de vaca e 27 de bufala. Deste total quatro eram produzidas em estabelecimentos com fiscalizacao de Servico de Inspecao Federal, nove em estabelecimentos com Servico de Inspecao Estadual, cinco eram de producao artesanal e as demais 48 amostras eram provenientes de producao informal. O DNA das amostras teste foi obtido por um protocolo de extracao e a reacao em cadeia pela polimerase foi realizada utilizando os oligoiniciadores B4 e B5 para detectar Brucella spp. e, os oligoiniciadores eri1 e eri2 para diferenciar cepa de infeccao a campo da cepa vacinal B19. Os resultados mostraram que 21,21% (14/66) das amostras foram positivas para Brucella spp., destas 21,43% (3/14) foram positivas para B. abortus cepa de campo e 7,14% (1/14) foi identificada como cepa vacinal B19. Concluiu-se que foi possivel identificar pela tecnica da PCR Brucella spp. em queijos na regiao amazonica, alem de diferenciar as cepas em amostra de B. abortus de infeccao a campo ou cepa vacinal B19.

Probiotics Bacillus toyonensis and Saccharomyces boulardii improve the vaccine immune response to Bovine herpesvirus type 5 in sheep

There have been significant efforts toward the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotics Bacillus toyonensis and Saccharomyces boulardii as a potential immune modulator to improve vaccine efficiency. Thirty, 5-month-old lambs were randomly grouped in three lots of 10 each and vaccinated at days 0, 21 and 42 of the experiment. They grazed on the same pasture and were fed ad libitum twice a day with commercial sheep feed supplemented with either B. toyonensis (1×106CFU/g of feed) or S. boulardii (1×107CFU/g of feed), or non-supplemented feed. The probiotic supplementation was suspended day 28; thereafter, the next 35days, they were fed with the same commercial feed as control group. Animals supplemented with probiotics showed a significant (p>0.001) increased seroconversions against BoHV-5, and higher neutralizing antibodies titres (p>0.05) to BoHV-5 than non-supplemented animals. At 63days of experiment, splenocytes from the supplemented sheep had higher mRNA transcription levels of cytokines IL-10 and IL-17A. These results suggest that these probiotics could provide a promising means of improving vaccine efficacy.

Segmentation of the buffalo meat consumer market in Belém, Pará, Brazil

The objective of this study was to identify different market segments for buffalo meat with target groups defined in the city of Belem, Para, Brazil. Paired samples of beef and buffalo meat were given to 447 volunteers, and a sensory analysis of the products was conducted. After the tasting, a questionnaire was administered to obtain demographic (age, educational level, marital status) and economic (household income) data as well as information on the habits of respondents and preferences regarding buffalo meat. The factors were estimated using the principal components method and the factors with characteristic roots greater than one were extracted. To check the suitability of the factorial model, Bartlett’s sphericity test and the Kaiser-Meyer-Olkin (KMO) test were used. A factor analysis was performed, identifying five factors with common variability dimensions. The study has helped identify four distinct market segments which, combined with a targeted marketing strategy, can be used to leverage the productive chain: I - Young and interested, II - Mixed and indifferent, III - Graduates and successful, and IV - Healthy women. All had positive responses to buffalo meat; clusters I and III stood out with regard to several indicators, especially those related to the preference for buffalo meat and the predisposition to purchase and include it in meals. The sensory analysis indicated that consumers showed a greater acceptance of buffalo meat based on the characteristics of flavour, colour, succulence and tenderness. The study helps to identify market segments with distinct features that, combined with a targeted marketing strategy, can be used to leverage the supply chain.

Palm kernel meal as additive in the elephant-grass silage

The objective of this study was to evaluate the effect of adding palm kernel meal (BP) as an additive in elephant grass silage. The chemical qualitative and fermentation were analysed in a completely randomized design with six treatments, one with no BP (C) and six replications, where the averages of the results of days of closing and opening were analysed by regression analysis. We collected samples at the time of ensiling and after 190 days of storage for evaluation of dry matter, crude protein, carbohydrates (total, non-fibrous, water-soluble, cellulose and hemicellulose), ash, lignin and ether extract. In aerobic phase, we collected samples for pH, ammoniacal nitrogen, fungi and yeasts. The elephant grass of this experiment showed values of 13.9% DM and 8.25% CP while the BP has 77.2% DM and 16.9% CP. The inclusion of BP had inhibited the growth of fungi and yeasts. The increase in the content of BP decreased the concentration of NH3-N. Higher concentrations of BP in silage were more stable, and the stability breaks in 72 hours, while the stability of C was 19 hours. Concentrations above 15% BP had no significant variations in pH in the aerobic phase. The inclusion of palm kernel meal at concentrations 10% to 15% can be used in silage of elephant grass, inhibiting undesired fermentation and making them more stable. Concentrations above this value may affect the nutritive value of silage by high lignin content of the additive.