Carl D. Paton

Skeletal muscle adaptation and performance responses to once a day versus twice every second day endurance training regimens

We determined the effects of a cycle training program in which selected sessions were performed with low muscle glycogen content on training capacity and subsequent endurance performance, whole body substrate oxidation during submaximal exercise, and several mitochondrial enzymes and signaling proteins with putative roles in promoting training adaptation. Seven endurance-trained cyclists/triathletes trained daily (High) alternating between 100-min steady-state aerobic rides (AT) one day, followed by a high-intensity interval training session (HIT; 8 x 5 min at maximum self-selected effort) the next day. Another seven subjects trained twice every second day (Low), first undertaking AT, then 1-2 h later, the HIT. These training schedules were maintained for 3 wk. Forty-eight hours before and after the first and last training sessions, all subjects completed a 60-min steady-state ride (60SS) followed by a 60-min performance trial. Muscle biopsies were taken before and after 60SS, and rates of substrate oxidation were determined throughout this ride. Resting muscle glycogen concentration (412 +/- 51 vs. 577 +/- 34 micromol/g dry wt), rates of whole body fat oxidation during 60SS (1,261 +/- 247 vs. 1,698 +/- 174 micromol.kg(-1).60 min(-1)), the maximal activities of citrate synthase (45 +/- 2 vs. 54 +/- 1 mmol.kg dry wt(-1).min(-1)), and beta-hydroxyacyl-CoA-dehydrogenase (18 +/- 2 vs. 23 +/- 2 mmol.kg dry wt(-1).min(-1)) along with the total protein content of cytochrome c oxidase subunit IV were increased only in Low (all P < 0.05). Mitochondrial DNA content and peroxisome proliferator-activated receptor-gamma coactivator-1alpha protein levels were unchanged in both groups after training. Cycling performance improved by approximately 10% in both Low and High. We conclude that compared with training daily, training twice every second day compromised high-intensity training capacity. While selected markers of training adaptation were enhanced with twice a day training, the performance of a 1-h time trial undertaken after a 60-min steady-state ride was similar after once daily or twice every second day training programs.

Tests of cycling performance.

Performance tests are an integral component of assessment for competitive cyclists in practical and research settings. Cycle ergometry is the basis of most of these tests. Most cycle ergometers are stationary devices that measure power while a cyclist pedals against sliding friction (e.g. Monark), electromagnetic braking (e.g. Lode), or air resistance (e.g. Kingcycle). Mobile ergometers (e.g. SRM cranks) allow measurement of power through the drive train of the cyclist’s own bike in real or simulated competitions on the road, in a velodrome or in the laboratory. The manufacturers’ calibration of all ergometers is questionable; dynamic recalibration with a special rig is therefore desirable for comparison of cyclists tested on different ergometers.For monitoring changes in performance of a cyclist, an ergometer should introduce negligible random error (variation) in its measurements; in this respect, SRM cranks appear to be the best ergometer, but more comparison studies of ergometers are needed. Random error in the cyclist’s performance should also be minimised by choice of an appropriate type of test. Tests based on physiological measures (e.g. maximum oxygen uptake, anaerobic threshold) and tests requiring self-selection of pace (e.g. constant-duration and constant-distance tests) usually produce random error of at least ~2 to 3%in the measure of power output. Random error as low as ~1% is possible for measures of power in ’all-out’ sprints, incremental tests, constant-power tests to exhaustion and probably also time trials in an indoor velodrome. Measures with such low error might be suitable for tracking the small changes in competitive performance that matter to elite cyclists.

Little effect of caffeine ingestion on repeated sprints in team-sport athletes.

PURPOSE The effect of caffeine ingestion on sprint performance is unclear. We have therefore investigated its effect on performance in a test that simulates the repeated sprints of team sports. METHODS In a randomized double-blind crossover experiment, 16 male team-sport athletes ingested either caffeine (6 mg.kg-1 of body mass) or a placebo 60 min before performing a repeated 20-m sprint test. The test consisted of 10 sprints, each performed within 10 s and followed by rest for the remainder of each 10 s. The caffeine and placebo trials followed a familiarization trial, and the time between consecutive trials was 2-3 d. To allow estimation of variation in treatment effects between individuals, nine subjects performed three more trials without a supplement 7-14 d later. We estimated the smallest worthwhile effect on sprint time in a team sport to be approximately 0.8%. RESULTS Mean time to complete 10 sprints increased by 0.1% (95% likely range -1.5 to 1.7%) with caffeine ingestion relative to placebo. Individual variation in this effect was a standard deviation of 0.7% (-2.7 to 2.9%). Time to complete the 10th sprint was 14.4% longer than the first; caffeine increased this time by 0.7% (-1.8 to 3.2%) relative to placebo, and individual variation in this effect was 2.4% (-3.4 to 4.9%). CONCLUSION The observed effect of caffeine ingestion on mean sprint performance and fatigue over 10 sprints was negligible. The true effect on mean performance could be small at most, although the true effects on fatigue and on the performance of individuals could be somewhat larger. Pending confirmatory research, team-sport athletes should not expect caffeine to enhance sprint performance.

Acute signalling responses to intense endurance training commenced with low or normal muscle glycogen

We have previously demonstrated that well‐trained subjects who completed a 3 week training programme in which selected high‐intensity interval training (HIT) sessions were commenced with low muscle glycogen content increased the maximal activities of several oxidative enzymes that promote endurance adaptations to a greater extent than subjects who began all training sessions with normal glycogen levels. The aim of the present study was to investigate acute skeletal muscle signalling responses to a single bout of HIT commenced with low or normal muscle glycogen stores in an attempt to elucidate potential mechanism(s) that might underlie our previous observations. Six endurance‐trained cyclists/triathletes performed a 100 min ride at ∼70% peak O2 uptake (AT) on day 1 and HIT (8 × 5 min work bouts at maximal self‐selected effort with 1 min rest) 24 h later (HIGH). Another six subjects, matched for fitness and training history, performed AT on day 1 then 1–2 h later, HIT (LOW). Muscle biopsies were taken before and after HIT. Muscle glycogen concentration was higher in HIGH versus LOW before the HIT (390 ± 28 versus 256 ± 67 μmol (g dry wt)−1). After HIT, glycogen levels were reduced in both groups (P < 0.05) but HIGH was elevated compared with LOW (229 ± 29 versus 124 ± 41 μmol (g dry wt)−1; P < 0.05). Phosphorylation of 5′AMP‐activated protein kinase (AMPK) increased after HIT, but the magnitude of increase was greater in LOW (P < 0.05). Despite the augmented AMPK response in LOW after HIT, selected downstream AMPK substrates were similar between groups. Phosphorylation of p38 mitogen‐activated protein kinase (p38 MAPK) was unchanged for both groups before and after the HIT training sessions. We conclude that despite a greater activation AMPK phosphorylation when HIT was commenced with low compared with normal muscle glycogen availability, the localization and phosphorylation state of selected downstream targets of AMPK were similar in response to the two interventions.

Combining Explosive And High-resistance Training Improves Performance In Competitive Cyclists

In several recent studies, athletes experienced substantial gains in sprint and endurance performance when explosive training or high-intensity interval training was added in the noncompetitive phase of a season. Here we report the effect of combining these 2 types of training on performance in the competitive phase. We randomized 18 road cyclists to an experimental (n = 9) or control (n = 9) group for 4–5 weeks of training. The experimental group replaced part of their usual training with twelve 30-minute sessions consisting of 3 sets of explosive single-leg jumps (20 for each leg) alternating with 3 sets of high-resistance cycling sprints (5 3 30 seconds at 60–70 min-1 with 30-second recoveries between repetitions). Performance measures, obtained over 2–3 days on a cycle ergometer before and after the intervention, were mean power in a 1- and 4-km time trial, peak power in an incremental test, and lactate-profile power and oxygen cost determined from 2 fixed submaximal workloads. The control group showed little mean change in performance. Power output sampled in the training sprints of the experimental group indicated a plateau in the training effect after 8–12 sessions. Relative to the control group, the mean changes (690% confidence limits) in the experimental group were: 1-km power, 8.7% (62.5%); 4-km power, 8.1% (64.1%); peak power, 6.8% (63.6); lactate-profile power, 3.7% (64.8%); and oxygen cost, 23.0% (62.6%). Individual responses to the training were apparent only for 4-km and lactate-profile power (standard deviations of 2.5% and 2.8%, respectively). The addition of explosive training and high-resistance interval training to the programs of already well-trained cyclists produces major gains in sprint and endurance performance, partly through improvements in exercise efficiency and anaerobic threshold.

Validity and reliability of a new field test (Carminatti's test) for soccer players compared with laboratory-based measures

Abstract The aim of this study was to assess the validity (Study 1) and reliability (Study 2) of a novel intermittent running test (Carminattis test) for physiological assessment of soccer players. In Study 1, 28 players performed Carminattis test, a repeated sprint ability test, and an intermittent treadmill test. In Study 2, 24 players performed Carminattis test twice within 72 h to determine test–retest reliability. Carminattis test required the participants to complete repeated bouts of 5 × 12 s shuttle running at progressively faster speeds until volitional exhaustion. The 12 s bouts were separated by 6 s recovery periods, making each stage 90 s in duration. The initial running distance was set at 15 m and was increased by 1 m at each stage (90 s). The repeated sprint ability test required the participants to perform 7 × 34.2 m maximal effort sprints separated by 25 s recovery. During the intermittent treadmill test, the initial velocity of 9.0 km · h−1 was increased by 1.2 km · h−1 every 3 min until volitional exhaustion. No significant difference (P > 0.05) was observed between Carminattis test peak running velocity and speed at VO2max (v-VO2max). Peak running velocity in Carminattis test was strongly correlated with v-VO2max (r = 0.74, P < 0.01), and highly associated with velocity at the onset of blood lactate accumulation (r = 0.63, P < 0.01). Mean sprint time was strongly associated with peak running velocity in Carminattis test (r = −0.71, P < 0.01). The intraclass correlation was 0.94 with a coefficient of variation of 1.4%. In conclusion, Carminattis test appears to be avalid and reliable measure of physical fitness and of the ability to perform intermittent high-intensity exercise in soccer players.

Effects of low- vs. high-cadence interval training on cycling performance.

Paton, CD, Hopkins, WG, and Cook, C. Effects of low- vs. high-cadence interval training on cycling performance. J Strength Cond Res 23(6): 1758-1763, 2009-High-resistance interval training produces substantial gains in sprint and endurance performance of cyclists in the competitive phase of a season. Here, we report the effect of changing the cadence of the intervals. We randomized 18 road cyclists to 2 groups for 4 weeks of training. Both groups replaced part of their usual training with 8 30-minute sessions consisting of sets of explosive single-leg jumps alternating with sets of high-intensity cycling sprints performed at either low cadence (60-70 min−1) or high cadence (110-120 min−1) on a training ergometer. Testosterone concentration was assayed in saliva samples collected before and after each session. Cycle ergometry before and after the intervention provided measures of performance (mean power in a 60-s time trial, incremental peak power, 4-mM lactate power) and physiologic indices of endurance performance (maximum oxygen uptake, exercise economy, fractional utilization of maximum oxygen uptake). Testosterone concentration in each session increased by 97% ± 39% (mean ± between-subject SD) in the low-cadence group but by only 62% ± 23% in the high-cadence group. Performance in the low-cadence group improved more than in the high-cadence group, with mean differences of 2.5% (90% confidence limits, ±4.8%) for 60-second mean power, 3.6% (±3.7%) for peak power, and 7.0% (±5.9%) for 4-mM lactate power. Maximum oxygen uptake showed a corresponding mean difference of 3.2% (±4.2%), but differences for other physiologic indices were unclear. Correlations between changes in performance and physiology were also unclear. Low-cadence interval training is probably more effective than high-cadence training in improving performance of well-trained competitive cyclists. The effects on performance may be related to training-associated effects on testosterone and to effects on maximum oxygen uptake.

Six minute walk distance is greater when performed in a group than alone

Objective: To investigate whether the distance covered in the six minute walk test was affected by walking with a group of others in comparison with performing the test alone. Methods: Eight healthy men (mean (SD) age 21.0 (0.9) years) and eight healthy women (mean (SD) age 20.8 (2.0) years) performed in random order two six minute walk tests either alone or in a group of four on two separate occasions one week apart. Results: Distance covered increased significantly from a mean of 653 (61) m in the individual male tests to 735 (79) m in the male group tests (p<0.05), and 616 (75) m in the individual female tests to 701 (54) m in the female group tests (p<0.01). The men increased the distance walked in six minutes by 12.5% and the women by 13.7% when they performed the test as a group. Conclusion: Performing the six minute walk test in a group facilitates its execution.

Effects of a Seven Day Overload-Period of High-Intensity Training on Performance and Physiology of Competitive Cyclists

Objectives Competitive endurance athletes commonly undertake periods of overload training in the weeks prior to major competitions. This investigation examined the effects of two seven-day high-intensity overload training regimes (HIT) on performance and physiological characteristics of competitive cyclists. Design The study was a matched groups, controlled trial. Methods Twenty-eight male cyclists (mean ± SD, Age: 33±10 years, Mass 74±7 kg, VO2 peak 4.7±0.5 L·min−1) were assigned to a control group or one of two training groups for seven consecutive days of HIT. Before and after training cyclists completed an ergometer based incremental exercise test and a 20-km time-trial. The HIT sessions were ∼120 minutes in duration and consisted of matched volumes of 5, 10 and 20 second (short) or 15, 30 and 45 second (long) maximal intensity efforts. Results Both the short and long HIT regimes led to significant (p<0.05) gains in time trial performance compared to the control group. Relative to the control group, the mean changes (±90% confidence limits) in time-trial power were 8.2%±3.8% and 10.4%±4.3% for the short and long HIT regimes respectively; corresponding increases in peak power in the incremental test were 5.5%±2.7% and 9.5%±2.5%. Both HIT (short vs long) interventions led to non-significant (p>0.05) increases (mean ± SD) in VO2 peak (2.3%±4.7% vs 3.5%±6.2%), lactate threshold power (3.6%±3.5% vs 2.9%±5.3%) and gross efficiency (3.2%±2.4% vs 5.1%±3.9%) with only small differences between HIT regimes. Conclusions Seven days of overload HIT induces substantial enhancements in time-trial performance despite non-significant increases in physiological measures with competitive cyclists.

Effects of caffeine chewing gum on race performance and physiology in male and female cyclists

Abstract This investigation reports the effects of chewing caffeinated gum on race performance with trained cyclists. Twenty competitive cyclists completed two 30-km time trials that included a maximal effort 0.2-km sprint each 10-km. Caffeine (~3–4 mg · kg−1) or placebo was administered double-blind via chewing gum at the 10-km point following completion of the first sprint. Measures of power output, oxygen uptake, heart rate, lactate and perceived exertion were taken at set intervals during the time trial. Results indicated no substantial differences in any measured variables between caffeine and placebo conditions during the first 20-km of the time trial. Caffeine gum did however lead to substantial enhancements (mean ± 90% confidence limits (CLs)) in mean power during the final 10-km (3.8% ± 2.3%), and sprint power at 30-km (4.0% ± 3.6%). The increases in performance over the final 10-km were associated with small increases in heart rate and blood lactate (effect size of 0.24 and 0.28, respectively). There were large inter-individual variations in the response to caffeine, and apparent gender related differences in sprint performance. Chewing caffeine gum improves mean and sprint performance power in the final 10-km of a 30-km time trial in male and female cyclists most likely through an increase in nervous system activation.