Carl G. Baker

Observed and calculated RF values of several synthetic peptides in paper chromatography

Abstract To supplement extensive information on peptides and amino acids, data were obtained on the paper-chromatographic behavior of 88 synthetic peptides and 29 amino acids in 15 solvent systems. Theoretical R F values were calculated for 46 peptides in 9 solvent system and were found to be in good agreement with the observed values.

Enzymatic oxidation of d- and l-α-hydroxy acids

Abstract 1. 1. The enzymatic oxidation of both optical isomers of straight-chain α-hydroxy acids from three through eight carbon atoms, α-hydroxyisovaleric, and α-hydroxyisocaproic acids, and of l -β-imidazole lactic acid has been studied. Several enzyme preparations from rat liver and kidney and from hog kidney were employed. 2. 2. The relative oxidation rates of the above compounds in the presence of a lyophilized, dialyzed hog kidney cortex supernatant preparation were determined. 3. 3. Addition of riboflavin monophosphate to a thoroughly dialyzed hog kidney cortex supernatant preparation produced an approximate doubling in the oxygen uptake. 4. 4. Experiments with washed particle and supernatant preparations from kidney and from liver suggest that there are at least two enzymes, distinct from lactic dehydrogenase and from the cyclophorase-racemase system, which catalyze the oxidation of α-hydroxy acids, one specific for the l - and the other for the d -isomer.