Carl Gustaf Hellerqvist

Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide from Salmonella typhimurium 395 ms

The structure of the O-specific side-chains of the cell-wall lipopolysaccharide of Salmonella typhimurium 395 MS has been investigated. Methylation analyses of the original lipopolysaccharide, of the material obtained on mild hydrolysis of the lipopolysaccharide with acid, and of a product obtained by acetalation of all of the, free hydroxyl groups in the lipopolysaccharide, followed by alkaline deacetylation, have provided the essential information in this study. The mixtures of sugars obtained on acid hydrolysis of the different methylated products were analysed, as alditol acetates, by g.l.c.-mass spectrometry. As a result of these studies, a detailed structure of the repeating unit of these side-chains is presented.

Structural studies of the o-specific side-chains of the cell-wall lipopolysaccharides from Salmonella newport and Salmonella kentucky

Abstract The structures of the O-specific side-chains of the cell-wall lipopolysaccharides (LPS) of Salmonella newport and Salmonella kentucky have been investigated. Methylation analyses of the original LPS, of the materials obtained on mild hydrolysis of the LPS with acid, and of a product obtained by acetalation of all of the free hydroxyl groups followed by deacetylation, have provided the essential information in this study. The mixtures of sugars obtained on acid hydrolysis of the different methylated products were analysed, as alditol acetates, by g.l.c.-mass spectrometry. As a result of these studies, detailed structures of the repeating units of these side-chains are presented.

Structural studies of the O-specific side-chains of the cell-wall lipopolysaccharide from Salmonella senftenberg

Abstract The structure of the O-specific side-chains of the cell-wall lipopolysaccharide (LPS) from Salmonella senftenberg , serogroup E 4 , has been investigated. From the methylation analysis of the LPS and of a partially hydrolysed product, the linkages could be identified and the order of the sugar residues assigned. The terminal, non-reducing sugar residue in the side-chains was also identified, thereby defining the “biological” repeating units in the side-chains. The anomeric nature of the different sugar residues was determined by polarimetric studies and by graded hydrolysis, followed by isolation and characterisation of a tetrasaccharide.

Structural studuies of the O-specific side-chains of the cell-wall lipopolysaccharide from Salmonella muenster

Abstract The structure of the O-specific side-chains of the cell-wall lipopolysaccharide (LPS) of Salmonella muenster has been investigated by methylation analysis of undegraded LPS and of a material obtained from it on graded hydrolysis, and by partial hydrolysis studies, including the isolation and characterisation of a trisaccharide. As a result of these studies, a detailed structure of the biological repeating unit in these side chains is presented.