Carl H. Hoffman

Polynucleotide phosphorylase covalently bound to cellulose and its use in the preparation of homopolynucleotides

Abstract A readily prepared, crude concentrate of polynucleotide phosphorylase (PNPase) has been covalently bound by the method of Porath to cyanogen bromide-activated cellulose; this is the first report of a synthesizing enzyme bound to a solid support. The bound enzyme had good activity and stability; more than forty consecutive polymerization cycles of nucleoside diphosphates have been carried out with a single preparation to give consistently good yields of stable polymers, and these were readily separated from the bound phosphorylase without tedious phenol extractions.