Carlos A. M. Rezende

Trypanocidal activity of lipophilic diamines and amino alcohols

Trypanocidal activity of a number of lipophilic diamines and amino alcohols was evaluated in vitro against Trypanosoma cruzi blood stream forms. Several of the studied compounds showed inhibition of T. cruzi growth. The most active ones were compounds 3, 4 and 5 with a IC₅₀ of 31.2 μg/mL, activity similar to the reference drug crystal violet.

Anthelmintic effects of alkylated diamines and amino alcohols against Schistosoma mansoni.

Polyamines are substances involved in many aspects of cell growth, division, and differentiation. Because of the metabolic differences between host cells and parasite cells, polyamine metabolism has been considered as a potential target for the chemotherapy of parasitic diseases. The aim of this work was to evaluate the schistosomicidal activity of different N-alkylated diamines (3a–3h), amino alcohols (4a–4d), and glycosylated amino alcohols (10a–10d). Compounds were prepared by synthetic methods and submitted to in vitro evaluation against adult worms of Schistosoma mansoni. At 100 μM, 3b, 3e, and 3h as well as 4a, 4b, 4d, 10a, 10b, and 10d resulted in 100% mortality of adult schistosomes. Compound 3d (12.5 to 100 μM) caused the death of 100% of both male and female adult schistosomes, while 3f (12.5 to 100 μM) resulted in 100% mortality of only male adult worms, whereas no mortality in female worms was observed. Compounds 3d and 3f were also able to reduce viability and decrease production of developed eggs in comparison with the negative control group. Diamines 3d and 3f may represent useful lead compounds for further optimization in order to develop new schistosomicidal agents.

The Cytogenetic Examination as a Tool for the Diagnosis of Chromosomal Disorders

Las anormalidades cromosomicas, clinicamente significativas, se presentan en aproximadamente 1% de los ninos nacidos vivos. Este trabajo tiene el objetivo de ofrecer a los pacientes y / o a sus familiares el servicio de la Clinica Integrada de la Salud de Uniara (Araraquara y Region), el examen de cariotipo (estudio citogenetico) para la confirmacion o la exclusion de sospecha de anomalias cromosomales diagnosticadas, asi como otorgar informacion (consejo genetico) para la prevencion de las posibles anomalias y / o la repeticion de estas. En un ano y cuatro meses fueron realizados 66 estudios de citogenetica en la Clinica Integrada de Uniara, dirigida por el Laboratorio de Citogenetica Humana de la misma institucion. En 44 pacientes (66,6%) los resultados fueron normales. En 22 (33,3%) de los examenes, se encontraron alteraciones, compatibles con alteraciones cromosomicas. La primera causa de anomalias cromosomica fue el sindrome de Down, totalizando 15 examenes (68,1%), la segunda causa fue el sindrome de Turner, con dos cariotipos (9,1%) en la forma mas importante 45, X. Por otra parte, se encontro que los sindromes de Eduards, de Patau, 3p-sindrome de Down, sindrome 4p-6p, diagnosticados en nuestro laboratorio, presentaban baja frecuencia de aparicion, representando el 22,7% de las anomalias estudiadas. Este trabajo permitio realizar un diagnostico preciso de las anomalias cromosomales, principalmente a traves de una tecnica de bajo costo, facil ejecucion y buena confiabilidad, tecnicas que estan disponibles para el examen citogenetico para la comunidad y asi contribuir de manera significativa en la calidad de vida de los pacientes.