Carlos H. Leal-Garza

Micronuclei in cervical smears and peripheral blood lymphocytes from women with and without cervical uterine cancer

Cervical cancer represents the second most common malignant neoplasia in women world-wide. In Mexico, cervical cancer is the most common female malignancy. It has been recently seen an increased frequencies of micronuclei (MN) lymphocytes and cervical epithelial cells of cervical cancer patients. The aim of this hospital-based unmatched case-control study was to investigate the association between progressive stages in development of cervical cancer and frequency of micronucleated cells in the cervical epithelium and peripheral lymphocytes of 40 women, grouped by disease stage. Women at the Obstetrics and Gynecology Hospital of the Instituto Mexicano del Seguro Social (IMSS) in Monterrey, Mexico were diagnosed and classified on the bases of the Papanicolaou (PAP) smear and colposcopy/biopsy into control, low-grade squamous intraepithelial lesions (LGSIL), high-grade squamous intraepithelial lesions (HGSIL), and invasive groups. Analysis of the MN data in both cell types revealed (a) homogeneity among women within each of the four groups with regard to MN frequency, (b) in general, a correlation between MN frequency and grade of cervical lesion, and (c) a positive linear trend between the MN frequency and increased cervical cancer risk. In conclusion, we suggest that MN are a useful biomarker of cancer risk. Nonetheless, these results should be validated by other researchers.

Sister chromatid exchanges in peripheral lymphocytes from women with carcinoma of the uterine cervix

Sister chromatid exchanges (SCE) are reciprocal exchanges between sister chromatids. It has been reported that in patients with cervical cancer, the frequency of SCE in peripheral lymphocytes is significantly higher than that in normal individuals; however, other studies have shown no significant difference. The aim of this unmatched case-control study was to compare the mean number of SCE per metaphase in lymphocytes from women with and without carcinoma of the cervix uteri. The SCE specimens were prepared by the fluorescence plus giemsa technique in peripheral lymphocytes from 28 women with carcinoma of cervix uteri and 28 controls. The mean number of SCE per metaphase in women with carcinoma of cervix uteri (7.80 +/- 1.05) was higher than the control group (6.98 +/- 1.13) (P < 0.05; t-test). This study had a statistical power of 0.80 and an alpha value of 0.05. This finding suggests that an increased number of SCE in peripheral lymphocytes is associated with cervical cancer. We consider that the lack of reported association of SCE and cervical cancer might be attributed to the none determination of the statistical power and sample size.

The effect of diphenylhydantoin on the frequency of micronuclei in bone-marrow polychromatic erythrocytes of mice.

Using the micronucleus test to evaluate the mutagenic effect of 5,5-diphenylhydantoin (DPH) on bone marrow polychromatic erythrocytes, male Balb-C mice were treated with the drug in single and multiple injection tests. A significant increase in the frequency of micronucleated polychromatic erythrocytes (MPE), P less than 0.05, was found when the mice received a single injection of DPH at doses of 0.5 and 1.0 mg/kg, and this frequency did not increase at higher doses. When mice were treated 3 times, at 24-h intervals, with 1.0 mg/kg of DPH, a significant increase in MPE was also observed (P less than 0.05) but this was lower than when they received a single injection of the same dose. A cytotoxic effect of NaOH, 0.1 N, which was used as solvent, was also observed either when alone or when DPH (1.0 mg/kg) was injected 3 times. This effect was comparable to the one produced by mitomycin C (MMC) at a dose of 0.5 mg/kg.

CHROMOSOMAL ABNORMALITIES AND POLYMORPHISMS IN MEXICAN INFERTILE MEN

A cross-sectional study was conducted to estimate the prevalence of chromosome abnormalities and normal variable chromosome features (polymorphisms) in infertile men from northeastern Mexico. Karyotyping was carried out in 326 men with diagnosis of infertility. The sperm counts showed 204 patients with oligozoospermia, 87 with azoospermia and 35 normozoospermia. Five patients with oligozoospemia and two with azoospermia presented chromosome abnormalities. Nonzoospermic men did not show chromosomal abnormalities. Polymorphisms of heterochromatin and satellite length showed a significant increased in oligozoospermic and azoospermic men with respect to normozoospermic men, respectively. This study reports the prevalence of chromosome abnormalities, polymorphisms of heterochromatin length, and polymorphisms in satellites in Mexican infertile men. The prevalence in this study was similar to other studies in world literature.

Gene frequencies and admixture estimates for ABO, Rho(D), and MN blood groups in persons with mono‐ and polyphletic surnames in monterrey, N.L., Mexico

A sample of 3,211 males and females insured by the Instituto Mexicano del Seguro Social (I.M.S.S.) residing in the Monterrey Metropolitan Area (MMA), northeastern Mexico, were selected by their monophyletic or polyphyletic surnames. ABO, Rho(D), and MN blood groups were determined, and phenotype and gene frequencies were estimated to study the genetic variation among populations with these surnames, to estimate the genetic contribution from their most important ancestral populations, both Spanish and Mexican Indians, and to compare genetic structure with other populations that have been reported from this MMA and other urban centers in Mexico with the hypothesis that the persons with monophyletic surnames are the closest descendants of the founders, who colonized the MMA between 1577 and 1596, and that they still conserve some degree of genetic isolation. The results indicate that the selected persons with monophyletic surnames are the closest to the Spanish, supporting the above hypothesis; on the other hand, persons with polyphyletic surnames are the closet to other Mestizo populations from central Mexico and the Mexican Indians. Hybrid persons with one monophyletic and one polyphyletic surname are closer to the monophyletics due to the fact that 90% of these polyphyletics originated in northeastern Mexico. It is concluded that, at present, the Mestizo population from the MMA is integrated by two subpopulations, one with monophyletic and the other with polyphyletic surnames. It is suggested that due to an increase in migration in Mexico, the Mestizo genetic structure of the MMA population will slowly become more uniform.