Carmelo Dazzi

Humic substances along the profile of two Typic Haploxerert

Abstract In Vertisols, organic matter contributes to soil colour by formation of organo-mineral complexes and affects morphological, physico-chemical, biological and biochemical properties. Turbation may affect the chemical and structural composition of the most stabilised fractions of soil organic matter (SOM), i.e., humic substances (HS). The objectives of this study were to: (1) characterise SOM in two Vertisols (V1 and V2) developed under Mediterranean climate in Italy, using some HS characteristics as indicators of SOM turnover in Vertisols, and (2) explore possible differences related to the pedomorphologic conditions of the area under which two soils have formed. SOM evolution along the soil profile was investigated by applying different techniques to chemically extracted HS. Humification parameters, thermal methods, such as Differential Scanning Calorimetry (DSC) and Thermogravimetry (TG) together with isoelectric focusing (IEF) were used. The results showed a significant difference in the chemical characteristics of SOM in the considered Vertisols. A higher amount of total and humified carbon was found in pedon V2. In particular, the results indicated an accumulation of humified compounds in deeper horizons of V2: this effect is particularly significant since both soils were under the same agricultural management and the same climatic conditions, but were different in physiografic position, slope and colour. The higher amount of not humified SOM in the pedon V1 could explain the differences found in the shape and size of soil structure between the two pedons. The different SOM evolution occurring in the two Vertisols apparently influenced the structure and composition of the HS fraction. Thermal analysis and isoelectric focusing data provided evidence of a different level of stabilisation of HS from the two Vertisols. The HS from pedon V2 have a higher molecular weight structure, which would imply a higher level of SOM stabilisation. Conversely, HS in the pedon V1 appeared to be richer in aliphatic and phenolic structures, which may imply the presence of less stabilised HS fractions. These findings agree well with the different water dynamics occurring in the two sites, which confirmed the influence exerted by water on chemical and structural composition of HS and, consequently, their effect in determining Vertisols colour. In conclusion, thermal and isoelectric focusing characterisation of HS appear to shed some light in understanding the structural complexity of Vertisols. The results obtained in the present study, if confirmed on a larger number of Vertisols, suggest the possibility of using HS as indicators of SOM turnover in relation to pedomorphology, use and management of soils.

Influence of inheritance and pedogenesis on heavy metal distribution in soils of Sicily, Italy

Abstract The recognition of the potential health hazards associated with heavy metals has focused attention on the levels and behaviours of these elements both in natural and contaminated environments. Various soil types developed from different parent materials in Sicily, Italy, have been analysed in order to compare heavy metal distribution under different geopedological conditions. Total metal concentrations, metal partitioning and distribution profiles are discussed in relation to the inheritance factor and pedogenic processes. Parent material composition largely influences the contents of heavy metals in most of the analysed soils, mainly differentiating pedons for their metal concentration on the basis of sedimentary and volcanic parent materials. However, an exception is represented by the carbonate-free Alfisols and Mollisols, which are markedly enriched in heavy metals with respect to the underlying limestones and calcarenites. On the basis of chemical and mineralogical results, supported by data from sequential extraction (SEP), heavy metal enrichment in these soils is associated with the formation of common to abundant Fe–Mn oxides and clay accumulation. For Mollisols there is evidence for a major role of organic matter in heavy metal retention in surface horizons. In the other pedons, pedogenic processes appear to mainly influence the redistribution of heavy metals throughout the soil horizons rather than affecting the total content. Argillopedoturbation seems to be responsible for a uniform distribution of heavy metals in Vertisol profiles on marly clays. Accumulation of heavy metals in the topsoil of Inceptisols on clays with gypsum and Entisols on gypsarenites and diatomites is caused partially by adsorption on organic matter and mainly by dissolution of primary gypsum and opal-A. A pedogenic influence is less evident in young Andisols whose heavy metal composition greatly reflects that of hawaiitic pyroclastic deposits interbedded within the profiles. However, a certain redistribution of Cu accumulated as metal–organic matter complexes is evidenced in the surface horizon by SEP.

Salinity and Bacterial Diversity: To What Extent Does the Concentration of Salt Affect the Bacterial Community in a Saline Soil?

In this study, the evaluation of soil characteristics was coupled with a pyrosequencing analysis of the V2-V3 16S rRNA gene region in order to investigate the bacterial community structure and diversity in the A horizon of a natural saline soil located in Sicily (Italy). The main aim of the research was to assess the organisation and diversity of microbial taxa using a spatial scale that revealed physical and chemical heterogeneity of the habitat under investigation. The results provided information on the type of distribution of different bacterial groups as a function of spatial gradients of soil salinity and pH. The analysis of bacterial 16S rRNA showed differences in bacterial composition and diversity due to a variable salt concentration in the soil. The bacterial community showed a statistically significant spatial variability. Some bacterial phyla appeared spread in the whole area, whatever the salinity gradient. It emerged therefore that a patchy saline soil can not contain just a single microbial community selected to withstand extreme osmotic phenomena, but many communities that can be variously correlated to one or more environmental parameters. Sequences have been deposited to the SRA database and can be accessed on ID Project PRJNA241061.

Compartmentalization of gypsum and halite associated with cyanobacteria in saline soil crusts

The interface between biological and geochemical components in the surface crust of a saline soil was investigated using X-ray diffraction, and variable pressure scanning electron microscopy in combination with energy dispersive X-ray spectrometry. Mineral compounds such as halite and gypsum were identified crystallized around filaments of cyanobacteria. A total of 92 genera were identified from the bacterial community based on 16S gene pyrosequencing analysis. The occurrence of the gypsum crystals, their shapes and compartmentalization suggested that they separated NaCl from the immediate microenvironment of the cyanobacteria, and that some cyanobacteria and communities of sulfur bacteria may had a physical control over the distinctive halite and gypsum structures produced. This suggests that cyanobacteria might directly or indirectly promote the formation of a protective envelope made of calcium and sulfur-based compounds.

Risk of classic Kaposi sarcoma with residential exposure to volcanic and related soils in Sicily.

PURPOSE Before AIDS, endemic (African) Kaposi sarcoma (KS) was noted to occur in volcanic areas and was postulated to result from dirt chronically embedded in the skin of the lower extremities. The primary cause of all KS types is KS-associated herpesvirus (KSHV) infection, but cofactors contribute to the neoplasia. We investigated whether residential exposure to volcanic or related soils was associated with the risk of classic Kaposi sarcoma (cKS) in Sicily. METHODS Risk of incident cKS (N=141) compared with population-based KSHV seropositive controls (N=123) was estimated for residential exposure to four types of soil, categorized with maps from the European Soil Database and direct surveying. Questionnaire data provided covariates. RESULTS Residents in communities high in luvisols were approximately 2.7 times more likely to have cKS than those in communities with no luvisols. Risk was not specific for cKS on the limbs, but it was elevated approximately four- to five-fold with frequent bathing or tap water drinking in communities with high luvisols. Risk was unrelated to communities high in andosols, tephra, or clay soils. CONCLUSIONS Iron and alumino-silicate clay, major components of luvisols, may increase cKS risk, but formal investigation and consideration of other soil types and exposures are needed.

Risk of classic Kaposi sarcoma with exposures to plants and soils in Sicily

BackgroundEcologic and in vitro studies suggest that exposures to plants or soil may influence risk of Kaposi sarcoma (KS).MethodsIn a population-based study of Sicily, we analyzed data on contact with 20 plants and residential exposure to 17 soils reported by 122 classic KS cases and 840 sex- and age-matched controls. With 88 KS-associated herpesvirus (KSHV) seropositive controls as the referent group, novel correlates of KS risk were sought, along with factors distinguishing seronegatives, in multinomial logistic regression models that included matching variables and known KS cofactors - smoking, cortisone use, and diabetes history. All plants were summed for cumulative exposure. Factor and cluster analyses were used to obtain scores and groups, respectively. Individual plants and soils in three levels of exposure with Ptrend ≤ 0.15 were retained in a backward elimination regression model.ResultsAdjusted for known cofactors, KS was not related to cumulative exposures to 20 plants [per quartile adjusted odds ratio (ORadj) 0.96, 95% confidence interval (CI) 0.73 - 1.25, Ptrend = 0.87], nor was it related to any factor scores or cluster of plants (P = 0.11 to 0.81). In the elimination regression model, KS risk was associated with five plants (Ptrend = 0.02 to 0.10) and with residential exposure to six soils (Ptrend = 0.01 to 0.13), including three soils (eutric regosol, chromic/pellic vertisol) used to cultivate durum wheat. None of the KS-associated plants and only one soil was also associated with KSHV serostatus. Diabetes was associated with KSHV seronegativity (ORadj 4.69, 95% CI 1.97 - 11.17), but the plant and soil associations had little effect on previous findings that KS risk was elevated for diabetics (ORadj 7.47, 95% CI 3.04 - 18.35) and lower for current and former smokers (ORadj 0.26 and 0.47, respectively, Ptrend = 0.05).ConclusionsKS risk was associated with exposure to a few plants and soils, but these may merely be due to chance. Study of the effects of durum wheat, which was previously associated with cKS, may be warranted.

Energy use in the A and B horizons of the soil under a pine and a cedar stand

Publisher Summary This chapter discusses a study that compares organic carbon content, availability of substrates, and microbial metabolism in two afforested sites in Sicily (Italy) developed under the same climatic and physical conditions but underlying different tree species. Given the key role of the soil microbiota in promoting energy exchanges and transformations in the soil profile, an analysis of the microbial activity and of organic carbon pools was performed on twenty profiles. The parameters considered in this study were based on microbial activity, organic carbon fractions, and kinetics of organic C mineralization. In the two systems studied, the competition for available energy among species of bacteria, fungi, and other organisms was driven mainly by differences in the plant species that form the leaf litter. As in all terrestrial ecosystems, the carbon balance is highly dependent on the living microbial biomass, which is responsible for a substantial amount of the carbon flux from terrestrial to atmospheric systems.

Soil humic acids formation and characteristics in a xeric Mollisol reforested with two tree species.

Publisher Summary The study discussed in this chapter examines the influence on humic acids (HAs) characteristics of different plant species— Pinus halepensis Miller and Cedrus atlantica (Endl) Carriere—used in the restoration of a Mollisol under xeric conditions. The area studied is in Sicily (Italy), and two stands were compared 40 years after planting. The differences among soil profile developments in the two stands were investigated by chemical and biochemical methods to understand the different levels at which the processes of pedogenesis acted in the study area. The chapter analyzes some chemical and spectroscopic characteristics of HAs, separated from two selected pedons. The results showed that the evolution of soils differed under the two tree stands, both in the formation of different humus forms and in different C storage in the forest floors. The HA chemical composition showed differences in the interactions between HAs and inorganic matter in relation to the tree species and depth. Because the pedogenetic factors were the same for the two pedons and the HA extracting procedure adopted was the same for all samples, the data suggest a different role for the microflora and plant coverage in the soil profile evolution of the two stands, which could address the pedogenesis process.

Saline soils in in Italy: distribution, ecological processes and socioeconomic issues

Soils are crucial for crop production and for the major ecosystem services. They preserve and sustain life. Salinity is one of the main soil threats that reduce soil fertility and affect the crop production. In recent times, a great attention has been paid to the general shortage of arable land, and to the increasing demand for ecological restoration of areas affected by secondary salinization processes. Microorganisms in these habitats may share a strategy, may have developed multiple adaptations for maintaining populations, and cope eventually to extreme conditions by altruistic or cooperative behavior for maintaining their population active. The understanding and the knowledge of the composition and distribution of microorganisms in natural habitats can be interesting for ecological reasons and it is important to develop new restoration strategy of salt-affected soils.

The “genetic erosion” of the soil ecosystem

Abstract This paper takes into consideration the influence of human activities on the loss of pedodiversity in a Mediterranean area due to large scale farming. In particular it examines the quantitative and qualitative soil changes in a period of 53 years (from 1955 to 2008) evaluating the loss of soil diversity at soil subgroups level of the USDA Soil Taxonomy system. The following indices were used: richness; Shannon’s diversity index; Simpson diversity index; Shannon’s evenness index; Simpson’s evenness index. In this case study, considering what we observed in time, the human intervention in soil transformation could increase the diversity in the landscape in an initial phase, but forwarding by large scale farming the result is a huge loss of pedodiversity in time, as diversity indices remarkably have shown. This analysis enabled identification of disappeared soil types, with their unique history of formation. In our opinion this strongly reflects a sort of “genetic erosion” of the soil types, resulting in a substantial weakening of the whole pedo-ecosystem.