Carmen Postolache

Nitrogen Removal by Riparian Buffers along a European Climatic Gradient: Patterns and Factors of Variation

AbstractWe evaluated nitrogen (N) removal efficiency by riparian buffers at 14 sites scattered throughout seven European countries subject to a wide range of climatic conditions. The sites also had a wide range of nitrate inputs, soil characteristics, and vegetation types. Dissolved forms of N in groundwater and associated hydrological parameters were measured at all sites; these data were used to calculate nitrate removal by the riparian buffers. Nitrate removal rates (expressed as the difference between the input and output nitrate concentration in relation to the width of the riparian zone) were mainly positive, ranging from 5% m−1 to 30% m−1, except for a few sites where the values were close to zero. Average N removal rates were similar for herbaceous (4.43% m−1) and forested (4.21% m−1) sites. Nitrogen removal efficiency was not affected by climatic variation between sites, and no significant seasonal pattern was detected. When nitrate inputs were low, a very large range of nitrate removal efficiencies was found both in the forested and in the nonforested sites. However, sites receiving nitrate inputs above 5 mg N L−1 showed an exponential negative decay of nitrate removal efficiency (nitrate removal efficiency = 33.6 e−0.11 NO3input, r2 = 0.33, P < 0.001). Hydraulic gradient was also negatively related to nitrate removal (r = −0.27, P < 0.05) at these sites. On the basis of this intersite comparison, we conclude that the removal of nitrate by biological mechanisms (for example, denitrification, plant uptake) in the riparian areas is related more closely to nitrate load and hydraulic gradient than to climatic parameters. n

Long-term changes of submerged macrophytes in the Lower Danube Wetland System

Transition towards hypertrophy has affected biodiversity and productivity of most aquatic and wetland systems in the Lower Danube Wetland System (LDWS) over the last two decades. The aquatic macrophytes have been deeply involved in ecosystem reorganization in these circumstances. Representative shallow lakes and channels located in the river floodplain and delta were studied in this period in terms of space distribution, diversity, species composition, primary production and main hydrogeomorphic features (morphometrical and physico-chemical parameters). Changes in submerged vegetation dynamics along two successive decades after 1980 included diminishing areas of about 50% in the Danube Delta, restructuring primary producers by suppressing aquatic weed in some lakes or parts of lakes and decreasing species richness to few populations with upright and floating growth strategy. Biomass production showed different trends, from severe reduction in some lakes and periods to marked increase in other ones, provided by a changed and simplified species structure, generally dominated by Ceratophyllum demersum L. and Potamogeton species. It was concluded that the eutrophication effects are maintaining, modulated by diverse response mechanisms developed by submerged macrophytes. Finally, the main lessons to be learned for the management of aquatic macrophyte-dominated systems in the framework of redesigning the LDWS structures are emphasized on the basis of a large scale and long-term prospect of the Danube River and Black Sea Basins.

Granular activated algae for wastewater treatment.

The study used activated algae granules for low-strength wastewater treatment in sequential batch mode. Each treatment cycle was conducted within 24 h in a bioreactor exposed to 235 μmol/m²/s light intensity. Wastewater treatment was performed mostly in aerobic conditions, oxygen being provided by microalgae. High removal efficiency of chemical oxygen demand (COD) was achieved (86-98%) in the first hours of the reaction phase, during which the indicators removal rate was 17.4 ± 3.9 mg O₂/g h; NH(4)(+) was removed during organic matter degradation processes with a rate of 1.8 ± 0.6 mg/g h. After almost complete COD removal, the (O⁺) remaining in the liquor was removed through nitrification processes promoted by the increase of the liquors oxygen saturation (O₂%), the transformation rate of NH4(+) into NO(3)(-) increasing from 0.14 ± 0.05 to 1.5 ± 0.4 mg NH4(+)/g h, along with an O₂% increase. A wide removal efficiency was achieved in the case of PO(4)(3)(-) (11-85%), with the indicators removal rate being 1.3 ± 0.7 mg/g h. In the provided optimum conditions, the occurrence of the denitrifying activity was also noticed. A large pH variation was registered (5-8.5) during treatment cycles. The granular activated algae system proved to be a promising alternative for wastewater treatment as it also sustains cost-efficient microalgae harvesting, with microalgae recovery efficiency ranging between 99.85 and 99.99% after granules settling with a velocity of 19 ± 3.6 m/h.

Ecological Significance of Nitrogen Cycling by Tubificid Communities in Shallow Eutrophic Lakes of the Danube Delta

Importance of tubificid populations on nitrogen cycle in two categories of shallow eutrophic lakes in the Danube Delta was quantitatively assessed for the 1992-1993 period. The structure of the primary producers in the studied lakes was used to discriminate between the two categories:(i) lakes dominated by macrophytes (A1) and (ii) lakes dominated by phytoplankton (A2). In both categories tubificid worms represented important fraction of the entire benthic community (35 and 32%, respectively, as number of individuals). They influence the sediment-water exchange of nutrients. The main processes involved are excretion of nutrients and their continuous release from sediments by molecular diffusion or through channels created by bioturbation. Inorganic nitrogen released from bottom sediments may regulate nitrogen load in the water body and thus, phytoplankton production. In 1992-1993, nitrogen stocks in tubificid biomass accounted for 5.3% in A1 lakes and 15.6% in A2 lakes of the amount stocked in phytoplankton, and only for 1.2 and 2.9% respectively, of the nitrogen load in water body. Nitrogen excretion rates ranged between 60.52 and 153.74 mg N m−2 year−1, and release rates from sediments between 378.26 and 960.87 mg N m−2 year−1, the lowest values being recorded for A2 category. Differences are related to tubificid biomass, structure and abundance of primary producers and to nutrient load in different ecosystems. Ratios between release rate of inorganic nitrogen by tubificid worms and sedimentation rate of organic nitrogen in the two categories of lakes were 8.3 and 6.4% respectively. Contribution of nitrogen released daily from sediments to the dissolved inorganic nitrogen load in the water column was less than 0.5%. However, in A1 and A2 lakes, the released nitrogen had a potential to sustain 24.74 and 8.01%, respectively, of the annual phytoplankton production. These values suggest the significance of tubificids in keeping the eutrophication process at a high level, especially during the periods when nitrogen is the main limiting factor for phytoplankton production.

A study of stakeholders' perspectives on multi-functional forests in Europe.

ABSTRACT This paper explores major opportunities and challenges of the development of multi-functional forestry in Europe by analysing existing attitudes of forestry stakeholders from Netherlands, Poland, Romania, Sweden and the UK. The research applies a combination of qualitative social science (participatory) approaches and quantitative tools as a means for understanding of a sensitive system of interactions between manifold socio-economic and nature protection activities in European forests that are managed for multiple purposes. Heterogeneity of attitudes of forestry stakeholders, concerning wood production, biodiversity conservation and provision of other ecosystem services, as well as of practical issues and benefits of multi-functional forestry to local communities are analysed by using the Q-methodology. Stakeholders perspectives regarding multi-functional forests and their sustainable management are identified through characterization of attitudinal groups and discursive analysis. Key factors influencing the attitudinal diversity are examined. Research outputs reveal the diversity of existing perspectives, together with stakeholders overall realisation of multifunctional forestry and their general support of sustainable forest management.

Nitrogen and phosphorous excretion rates by tubificids from the Prahova River (Romania)

Nitrogen and phosphorous exchange at the water–sediment interface is controlled both by complex physico-chemical factors and biological processes. Zoobenthos excretion is one of the most important processes in the mineralization of sedimented organic mater. In polluted freshwaters, tubificid worms are among the dominant components of the benthic community. Rates of ammonium and inorganic phosphate excretion by tubificids were experimentally assessed. They were related to the tubificid abundance in a stream ecosystem polluted with municipal and industrial wastewater. The relationship between these rates and temperature were investigated within the range of 4–23xa0°C. Relatively constant excretion rates were obtained for both nutrients in the first 8xa0h of excretion, ranging between 0.076 and 0.226xa0μg Nxa0mgxa0d.w.−1xa0h−1 and 0.0065–0.01xa0μg Pxa0mgxa0d.w.−1xa0h−1, respectively. Q10 values of 2.52 for ammonium and 1.31 for phosphate were calculated. If we presume that all excreta eventually enters the water column, then we can calculate that these invertebrates potentially add 39.17xa0mg Nxa0m−2xa0day−1 and 0.49xa0mg Pxa0m−2xa0day−1. These values accounts for 17.16 and 7.56% of the nutrient load in the river water, respectively.

Dairy wastewater treatment using an activated sludge–microalgae system at different light intensities

A microalgae-bacteria system was used for dairy industry wastewater treatment in sequenced batch mode in a photobioreactor. The research investigated the influence of two light intensities: 360 and 820 μmol m(-2)s(-1) on treatment performances, microalgal cell recovery and dynamics of the protozoan community. Results showed that the light intensity of 360 μmol m(-2)s(-1) was found to be insufficient to support photosynthetic activity after the increase of bacterial biomass leading to the decrease of organic matter and ammonium removal efficiencies from 95 to 78% and 95 to 41%, respectively. Maximum microalgal cells recovery was about 63%. Continuous modification in the protozoan community was also noticed during this test. Increasing the light intensity to 820 μmol m(-2)s(-1) led to better microalgal cells recovery (up to 88%) and improved treatment performances. However, the decrease of protozoan richness to small flagellates and free-swimming ciliates was noticed. Moreover, the developed protozoan trophic network was found to be different from that identified in the conventional activated sludge system. The study emphasized that high increase of bacterial biomass promoted in nutrient- and organic matter-rich wastewater can strongly affect the treatment performances as a result of the shadow effect produced on the photoautotrophic microalgae aggregates.

What is socio-ecological research delivering? A literature survey across 25 international LTSER platforms

With an overarching goal of addressing global and regional sustainability challenges, Long Term Socio-Ecological Research Platforms (LTSER) aim to conduct place-based research, to collect and synthesize both environmental and socio-economic data, and to involve a broader stakeholder pool to set the research agenda. To date there have been few studies examining the output from LTSER platforms. In this study we enquire if the socio-ecological research from 25 self-selected LTSER platforms of the International Long-Term Ecological Research (ILTER) network has produced research products which fulfil the aims and ambitions of the paradigm shift from ecological to socio-ecological research envisaged at the turn of the century. In total we assessed 4983 publically available publications, of which 1112 were deemed relevant to the socio-ecological objectives of the platform. A series of 22 questions were scored for each publication, assessing relevance of responses in terms of the disciplinary focus of research, consideration of human health and well-being, degree of stakeholder engagement, and other relevant variables. The results reflected the diverse origins of the individual platforms and revealed a wide range in foci, temporal periods and quantity of output from participating platforms, supporting the premise that there is a growing trend in socio-ecological research at long-term monitoring platforms. Our review highlights the challenges of realizing the top-down goal to harmonize international network activities and objectives and the need for bottom-up, self-definition for research platforms. This provides support for increasing the consistency of LTSER research while preserving the diversity of regional experiences.

Effects of anthropogenic fragmentation on primary productivity and soil carbon storage in temperate mountain grasslands

Habitat fragmentation is one of the most severe anthropogenic pressures exerted on ecosystem’s biodiversity. Empirical studies to date focused with an overriding interest on the effects of habitat loss or habitat fragmentation per se on species richness patterns detrimental to biogeochemical processes. To account for changes in ecosystem fluxes, we investigated how anthropogenic fragmentation affects primary productivity and carbon storage in temperate mountain grasslands. A field study was conducted to assess the influence of grassland isolation on soil carbon stocks, N availability, species biomass, and plant functional groups distribution. We tested the hypothesis that increased isolation of grassland, within the land cover, decreases soil carbon stocks, and available N nutrient as well as aboveground biomass. Soil carbon concentration decreased with isolation but increased near the forest edge. We found significant differences in aboveground biomass distribution and relative contribution of plant functional groups between isolation conditions. The magnitude of edge effect on carbon stocks, N availability, and primary productivity intensified with increasing isolation as a consequence of the additive influence of edges. Our study reveals that the potential creation of artificially isolated patches diminished primary productivity, N availability, and C stocks. However, in highly managed landscapes, grazing pressure is an additional factor that changes biomass and nutrients patterns. We emphasize that spatial configuration of the landscape has a major role in modulating ecological flows and ecosystem service supply, in addition to changes in species richness.

Planar Chromatographic and Electrophoretic Study of Thermally Induced Conformational Modifications of Protein Structure

It is well known that the structure of BSA explains its capacity to interact both hydrophobically and hydrophilically with other biochemical species. In the native state the BSA molecule takes such a conformation that the polar residues of amino-acids are oriented towards the outside the molecule and the non-polar residues are directed prevalently towards the inside of the molecule. The main effect of heat on the conformation of the albumin is supposed to consist in the redirection of the amino acid moieties which involves a change in the hydrophobic–hydrophilic balance. A sequence of conformational modifications is assumed to result from different refolding processes of thermally denatured BSA. This leads, because of the large number of degrees of freedom of the macromolecule, from the largely featureless ensemble of denatured conformations to structures modified do different extents compared with the one structure of the native albumin molecule [1]. In this way the capacity of the protein to interact or bind with other species is modified, as is well observed in its chromatographic and electrophoretic behavior. Our paper deals with use of these techniques to study the effect of the refolding process on thermally denatured BSA.