Caroline Bohnen de Matos

Atividade antifúngica do óleo essencial de Origanum vulgare frente a Malassezia pachydermatis

The aim of this study was to evaluate the in vitro antifungal activity of essential oil of Origanum vulgare against clinical isolates of Malassezia pachydermatis. The dried leaves of O. vulgare were purchased from a commercial distributor with certified quality and origin and referred for essential oil extraction and chromatography. The technique for in vitro testing was microdilution (CLSI M27A3) with modifications to phytochemicals and M. pachydermatis. The essential oil of O. vulgare was tested at concentrations from 28 to 0.87mg/mL in Sabouraud broth diluted with 1% of tween 80. All isolates were tested in duplicate. In the chromatographic analysis of the essential oil 12 compounds were identified, and thymol, α-terpinene, 4-terpineol were the major compounds. The MIC and the MFC of the 42 isolates of M. pachydermatis ranged from <0.87 to 7mg/mL with MIC50 and MIC90 values of 1.18 and 3.28 mg/mL, respectively. With this study it was concluded that M. pachydermatis is sensible to O. vulgare essential oil even at low concentrations. Thus, the essential oil of O. vulgare is presented as bioprospecting in the promising new drugs for the treatment of otitis and dermatitis in small animal clinic.

Dermatite multifatorial em um canino

A clinical case of dermatitis multifactorial in a dog was reported, involving Demodex canis, Sporothrix schenckii, and bacteria of the genera Staphylococcus and Streptococcus. The animal, male, Pit Bull, with a year and three months of age had soaked lesions, was hyperemic, scaly and had an ulcer in the neck and chest. Laboratory tests confirmed the diagnosis of concomitant infection by fungi, bacteria and parasites. Antimicrobial therapy with moxidectin, itraconazol and amoxicillin was effective, with remission of clinical signs after 30 days of treatment. This report emphasizes the importance of an accurate diagnosis, which was crucial to establish the correct treatment and recovery of the animal.

Germicidal activity of plant extracts of Origanum vulgare and Rosmarinus officinalis against Sporothrix schenckii complex

The literature shows that is a resistance by the microorganisms against commercial products used for the disinfection of contaminated environments. Due to this, the aim of this study was to evaluate the activity of the tincture, aqueous extract (decoction and infusion) and hydroalcoholic extract of Origanum vulgare and Rosmarinus officinalis, as an alternative to disinfectants, using the technique of direct exposure, against Sporothrix schenckii complex. For this, the direct exposure tests, with fungal isolates from clinical cases pertaining to dogs and cats with sporotrichosis, were used to evaluate the sensitivity of Sporothrix spp. against plants extracts, with the action evaluated in the times of 5, 10 and 15 minutes of exposure . As a positive control, it was used a sterile water solution containing innocuous and disinfectants used to control was sodium hypochlorite alcohol and 70°GL. As a result, it can be shown the sensitivity of the fungal isolates front to tincture of Origanum vulgare and Rosmarinus officinalis and hydroalcoholic extract of Origanum vulgare at all times of exposure, in 10% concentration of the extract.