Caroline Rego Rodrigues

Prevalência do uso de drogas psicotrópicas em unidades da polícia militar

The present study aimed to verify the prevalence of psychoactive drug use (amphetamines, methamphetamines, cannabinoids, cocaine, opioids and benzodiazepines) among military police officers in the state of Goiás. Data were obtained from urine samples voluntarily provided by the officers participating in the study, who were informed of the study methods and signed a free and informed consent form. The samples were subject to screening analysis by immunochromatography (Multi-DrugOneStep Test®), with positive tests confirmed by gas chromatography- mass spectrometry (GC-MS) and data analyzed by descriptive statistics. The results indicated the presence of the following drugs: amphetamines (0.33%), cannabinoids (0.67%) and benzodiazepines (1.34%); 97.66% showed negative results. The positive cases were distributed as follows: benzodiazepines (57.1%); cannabinoids (28.6%) and amphetamines (14.3%). In conclusion, the detection of psychoactive substances in voluntary sampling of military police officers indicates the need to implement drug testing among active military officers and preventive public policies aimed at eliminating the abusive consumption of psychotropic drugs.Este trabalho objetivou verificar a prevalencia do uso de drogas psicoativas (anfetamina, metanfetamina, canabinoides, cocaina, opioides e benzodiazepinicos) entre policiais militares do Estado de Goias. Os dados foram obtidos a partir de amostras de urina cedidas voluntariamente pelos policiais participantes da pesquisa, os quais foram esclarecidos em relacao a metodologia do estudo e assinaram o TCLE. As amostras foram submetidas a analise de triagem por imunocromatografia (Multi-DrugOneStep Test®) e, aquelas positivas, foram confirmadas por cromatografia em fase gasosa acoplada a espectrometria de massa. Os dados foram analisados por estatistica descritiva. Os resultados apontaram a presenca das seguintes drogas: anfetaminas (0,33%); canabinoides (0,67%); benzodiazepinicos (1,34%); 97,66% foram resultados negativos. A distribuicao dos casos positivos foi: benzodiazepinicos (57,1%); canabinoides (28,6%) e anfetaminas (14,3%). Conclui-se que os achados de substâncias psicoativas na amostra de policiais militares voluntarios chama a atencao sobre a necessidade da implantacao de testes de drogas no efetivo da policia militar e de politicas publicas preventivas, que visem evitar as consequencias do consumo abusivo de psicotropicos.

Monitorización terapéutica de la digoxina en pacientes con insuficiencia cardíaca: comparación entre dos métodos analíticos

Objective: To compare two analytical techniques used in the determination of plasma digoxin (LC-MS/MS and immunoassay) and to verify which one better answer the need of the clinical monitoring routine of patients with cardiac heart failure. Method: The clinical findings in 15 cardiac heart failure (CHF) outpatients of the Cardiac Heart Service of the Goias Federal University Clinical Hospital were investigated. Blood samples of the patients were collected and analysed by Immunoassay and by Liquid Chromatography coupled to Mass Spectrometry (LC-MS/MS). Results: The results of the statistic test (Student p = 0,05) showed a significant difference between the analytical methods: immunoassay concentrations were higher than the concentrations determined by LCMS/MS. The explanation may be because immunoassay method measures digoxin plus other metabolites and endogenous substances, while the LC-MS/MS method measures only the digoxin molecule. None of the patients, showed relevant clinical data suggestive of digitalis intoxication, even several drugs with potential interaction were associated with treatment. Conclusion: It was concluded, therefore, that LC-MS/MS me thod is safer, more selective and specific than immunoassay, being an option for therapeutic drug monitoring of digoxin, since the reference values would be obtain for digoxinemia by LC-MS/MS. ORIGINALES Monitorización terapéutica de la digoxinemia en pacientes con insuficiencia cardiaca: comparación entre dos métodos analíticos Isabel Cristina Medeiros Barros1, Elis Marra da Madeira Freitas1, Salvador Rassi2, Wilsione José Carneiro1, Caroline Rego Rodrigues1, Tereza Cristina de Deus Honório1, Leonardo de Souza Teixeira3, Vania Rodríguez1 y Luiz Carlos da Cunha1 1Núcleo de Estudos e Pesquisas Tóxico-Farmacológicas. NEPET. Faculdade de Farmácia da Universidade Federal de Goiás. 2Faculdade de Medicina da Universidade Federal de Goiás. 3Instituto de Ciências Farmacêuticas. ICF. * Autor para correspondencia. Correo electrónico: vaniaro2009@gmail.com (Vania Cristina Rodríguez Salazar) Recibido el 14 de junio de 2013; aceptado el 5 de diciembre de 2013. DOI: 10.7399/FH.2014.38.1.1099 Farm Hosp. 2014;38(1):38-43 Farm Hosp. 2014;38(1):38-43Objective: To compare two analytical techniques used in the determination of plasma digoxin (LC-MS/MS and immunoassay) and to verify which one better answer the need of the clinical monitoring routine of patients with cardiac heart failure. Method: The clinical findings in 15 cardiac heart failure (CHF) outpatients of the Cardiac Heart Service of the Goias Federal University Clinical Hospital were investigated. Blood samples of the patients were collected and analysed by Immunoassay and by Liquid Chromatography coupled to Mass Spectrometry (LC-MS/MS). Results: The results of the statistic test (Student p = 0,05) showed a significant difference between the analytical methods: immunoassay concentrations were higher than the concentrations determined by LCMS/ MS. The explanation may be because immunoassay method measures digoxin plus other metabolites and endogenous substances, while the LC-MS/MS method measures only the digoxin molecule. None of the patients, showed relevant clinical data suggestive of digitalis intoxication, even several drugs with potential interaction were associated with treatment. Conclusion: It was concluded, therefore, that LC-MS/MS me thod is safer, more selective and specific than immunoassay, being an option for therapeutic drug monitoring of digoxin, since the reference values would be obtain for digoxinemia by LC-MS/MS.