Carrington S. Cobbs

Immunosorbent chromatography of sea nettle (Chrysaora quinquecirrha) venom and characterization of toxins

A lethal toxic fraction from nematocysts of the sea nettle (Chrysaora quinquecirrha) fishing tentacle was partially purified by immunochromatography using an immobilized monoclonal antibody column. Elution from the immunosorbent was accomplished under mild conditions which conserved the biological activity of the toxin. The isolated fraction, which contained two purified protein bands with molecular weights of 100,000 and 190,000 daltons on SDS polyacrylamide gels, was both cardiotoxic and neurotoxic and exhibited an intravenous lethal activity (LD50) of 0.37 microgram/g in mice.

Sea nettle (Chrysaora quinquecirrha) lethal factor: effect on black lipid membranes

A lethal toxin from the fishing tentacles of the sea nettle, Chrysaora quinquecirrha, affected ion permeability in black lipid membranes by producing monovalent cation channels. These channels appeared to measure approximately 31 pS.

Sea nettle Chrysaora quinquecirrha) nematocyst venom hemagglutinins

Abstract 1. Sea nettle nematocyst venom contains hemagglutinins specific for human and rabbit erythrocytcs. 2. These hemagglutinins exist as multiple molecular forms based on their behavior on DEAE cellulose and SDS-PAGE. 3. Hemagglutinin activity was not inhibited by several simple mono- and disaccharides, but was inhibited by dextran sulfate and certain complex polysaccharides.