Cary L. Rivard

Rootstocks shape the rhizobiome: Rhizosphere and endosphere bacterial communities in the grafted tomato system

Root-associated microbes are critical to plant health and performance, although understanding of the factors that structure these microbial communities and theory to predict microbial assemblages are still limited. Here we use a grafted tomato system to study the effects of rootstock genotypes and grafting in endosphere and rhizosphere microbiomes that were evaluated by sequencing 16S rRNA. We compared the microbiomes of nongrafted tomato cultivar BHN589, selfgrafted BHN589, and BHN589 grafted to Maxifort or RST-04-106 hybrid rootstocks. OTU-based bacterial diversity was greater in Maxifort compared to nongraft controls, whereas bacterial diversity in the controls (selfgraft and nongraft) and the other rootstock (RST-04-106) was similar. Grafting itself did not affect bacterial diversity; diversity in the selfgraft was similar to the nongraft. Bacterial diversity was higher in the rhizosphere than in the endosphere for all treatments. However, despite the lower overall diversity, there was a greater number of differentially abundant OTUs (DAOTUs) in the endosphere, with the greatest number of DAOTUs associated with Maxifort. In a PERMANOVA analysis, there was evidence for an effect of rootstock genotype on bacterial communities. The endosphere-rhizosphere compartment and study site explained a high percentage of the differences among bacterial communities. Further analyses identified OTUs responsive to rootstock genotypes in both the endosphere and the rhizosphere. Our findings highlight the effects of rootstocks on bacterial diversity and composition. The influence of rootstock and plant compartment on microbial communities indicates opportunities for the development of designer communities and microbiome-based breeding to improve future crop production. Importance Understanding factors that control microbial communities is essential for designing and supporting microbiome-based agriculture. In this study, we used a grafted tomato system to study the effect of rootstock genotypes and grafting on bacterial communities colonizing the endosphere and the rhizosphere. Comparing the bacterial communities in control treatments (nongraft and selfgraft plants) with the hybrid rootstocks used by farmers, we evaluated the effect of rootstocks on overall bacterial diversity and composition. These findings indicate the potential for using plant genotype to indirectly select bacterial taxa. In addition, we identify taxa responsive to each rootstock treatments, which may represent candidate taxa useful for biocontrol and in biofertilizers.

Day-neutral Strawberries for High Tunnel Production in the Central United States

Specialty crop production in high tunnel systems has greatly expanded in the central United States. Strawberry (Fragaria ·ananassa) may be a viable highvalue crop for high tunnel growers, but fall-planted production systems have a high opportunity cost in regard to winter production space. This study investigates the feasibility of spring-planted day-neutral strawberry cultivars in a high tunnel production system in Kansas. Furthermore, the goals of this report are to identify day-neutral cultivars that are successful in this production system and investigate the utility of evaporative cooling (EC), as they relate to fruit yield and marketability as well as incidence of gray mold (caused by Botrytis cinerea). High tunnel trials were conducted at the Kansas State University Olathe Horticulture Research and Extension Center during 2014 and 2015. Six commercially available cultivars were evaluated: Albion, Evie 2, Monterey, Portola, San Andreas, and Seascape in both years. Mature fruit (90% to 100% red) were harvested twice weekly for total andmarketable (fruit with no defects) fruit yield. The results indicate that ‘Portola’ had the highest total fruit weight in both years at 0.60 and 0.51 kg/plant, respectively, and was significantly higher than ‘Monterey’, ‘Albion’, and ‘San Andreas’ in both years (P < 0.05). In 2014, ‘Portola’, ‘San Andreas’, and ‘Albion’ produced the largest total fruit average weight (grams/fruit) and were significantly larger than ‘Seascape’ and ‘Evie 2’ (P < 0.05). In 2015, ‘Portola’ had significantly larger fruit than all the other cultivars except SanAndreas (P < 0.05).Marketability percent by weight ranged from 76.5% to 88.6% across both years and the highest marketability was observed with ‘Albion’ (89% and 83%) and ‘Monterey’ (85% and 84%) in 2014 and 2015, respectively. An examination of fruit production during the early, mid-, and late seasons was used to determine seasonal dynamics of each cultivar and the high tunnel system. The overall trend was that total fruit weight was highest during the midseason and total average fruit weight was largest in the early season. However, ‘San Andreas’ and ‘Seascape’ had similar levels of production between the early and midseason and had the lowest level of production in the midseason, particularly in the 2015 trial. In our trials, the use of EC did not affect fruit weight or gray mold incidence on strawberry fruit. Based on the crop productivity observed in our study, this production system has the potential to extend the season for strawberry growers in the central United States or provide a high-value rotational crop for existing high tunnel growers that does not require winter production space.