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Dive into the research topics where Catalina von Bilderling is active.

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Featured researches published by Catalina von Bilderling.


Journal of Cell Biology | 2015

Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate.

Pehuén Pereyra Gerber; Mercedes Cabrini; Carolina Jancic; Luciana Paoletti; Claudia Banchio; Catalina von Bilderling; Lorena Sigaut; Lía I. Pietrasanta; Gabriel Duette; Eric O. Freed; Geneviève de Saint Basile; Catarina Moita; Luis F. Moita; Sebastian Amigorena; Philippe Benaroch; Jorge Geffner; Matias Ostrowski

Rab27a controls the recruitment of phosphatidylinositol 4-kinase type 2α from endosomes to the plasma membrane, which promotes high levels of PI(4)P, fuels PI(4,5)P2 production, and favors the recruitment of Pr55Gag and HIV-1 assembly.


Optics Express | 2009

Molecular orientation in self-assembled multilayers measured by Second Harmonic generation using femtosecond pulses.

Catalina von Bilderling; Mario Tagliazucchi; Ernesto J. Calvo; Andrea V. Bragas

We present measurements of the optical second-harmonic generation in self assembled multilayer films of PAZO/PAH polymers with the aim to investigate molecular order in the layer-by-layer architecture. The experiments are performed in transmission, using a femtosecond Ti:Sa pulsed laser, which allows a more accurate determination of the amplitude of the second harmonic signal, without interference fringes usually present in nanosecond experiments. We found that the first bilayer, in contact with the substrate, presents a broad distribution of the orientation of the molecules, while the addition of successive bilayers (up to 12) produces ordering of the molecules with a small tilt angle respect to the surface normal. This result, together with the monotonic increment of the second harmonic signal with the number of layers indicates that the molecules grow orderly assembled in these films.


Scientific Reports | 2018

Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Nicolas Unsain; Martin D. Bordenave; Gaby F. Martinez; Jalil Sami; Catalina von Bilderling; Federico M. Barabas; Luciano A. Masullo; Aaron D. Johnstone; Philip A. Barker; Bisbal Mariano; Fernando D. Stefani; Alfredo Cáceres

Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axonal degeneration. We studied the actin cytoskeleton in an in vitro model of developmental pruning induced by trophic factor withdrawal (TFW). We found that F-actin decrease and growth cone collapse (GCC) occur early after TFW; however, treatments that prevent axonal fragmentation failed to prevent GCC, suggesting independent pathways. Using super-resolution (STED) microscopy we found that the axonal actin/spectrin membrane-associated periodic skeleton (MPS) abundance and organization drop shortly after deprivation, remaining low until fragmentation. Fragmented axons lack MPS (while maintaining microtubules) and acute pharmacological treatments that stabilize actin filaments prevent MPS loss and protect from axonal fragmentation, suggesting that MPS destruction is required for axon fragmentation to proceed.


Scientific Reports | 2018

Author Correction: Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Nicolas Unsain; Martin D. Bordenave; Gaby F. Martinez; Sami Jalil; Catalina von Bilderling; Federico M. Barabas; Luciano A. Masullo; Aaron D. Johnstone; Philip A. Barker; Mariano Bisbal; Fernando D. Stefani; Alfredo Cáceres

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper


Journal of Colloid and Interface Science | 2018

Reversible modulation of the redox activity in conducting polymer nanofilms induced by hydrophobic collapse of a surface-grafted polyelectrolyte

Gonzalo E. Fenoy; Juan M. Giussi; Catalina von Bilderling; Eliana Maza; Lía I. Pietrasanta; Wolfgang Knoll; Waldemar A. Marmisollé; Omar Azzaroni

We present the covalent modification of a Pani-like conducting polymer (polyaminobenzylamine, PABA) by grafting of a polyelectrolyte brush (poly [2-(methacryloyloxy)-ethyl-trimethylammonium chloride], PMETAC). As PABA has extra pendant amino moieties, the grafting procedure does not affect the backbone nitrogen atoms that are implicated in the electronic structure of the conducting polymers. Moreover, perchlorate anions interact very strongly with the quaternary ammonium pendant groups of PMETAC through ion pairing. Therefore, the grafting does not only keep the electroactivity of PABA in aqueous solutions but it adds the ion-actuation properties of the PMETAC brush to the modified electrode as demonstrated by contact angle measurements and electrochemical methods. In this way, the conjugation of the electron transfer properties of the conducting polymer with the anion responsiveness of the integrated brush renders perchlorate actuation of the electrochemical response. These results constitute a rational integration of nanometer-sized polymer building blocks that yields synergism of functionalities and illustrate the potentialities of nanoarchitectonics for pushing the limits of soft material science into the nanoworld.


Scientific Reports | 2018

Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress

Lorena Sigaut; Catalina von Bilderling; Micaela Bianchi; Juan Eduardo Burdisso; Laura Gastaldi; Lía I. Pietrasanta

Mechanical stimuli play a key role in many cell functions such as proliferation, differentiation and migration. In the mammary gland, mechanical signals such as the distension of mammary epithelial cells due to udder filling are proposed to be directly involved during lactation and involution. However, the evolution of focal adhesions -specialized multiprotein complexes that mechanically connect cells with the extracellular matrix- during the mammary gland development, as well as the influence of the mechanical stimuli involved, remains unclear. Here we present the use of an equibiaxial stretching device for exerting a sustained normal strain to mammary epithelial cells while quantitatively assessing cell responses by fluorescence imaging techniques. Using this approach, we explored changes in focal adhesion dynamics in HC11 mammary cells in response to a mechanical sustained stress, which resembles the physiological stimuli. We studied the relationship between a global stress and focal adhesion assembly/disassembly, observing an enhanced persistency of focal adhesions under strain as well as an increase in their size. At a molecular level, we evaluated the mechanoresponses of vinculin and zyxin, two focal adhesion proteins postulated as mechanosensors, observing an increment in vinculin molecular tension and a slower zyxin dynamics while increasing the applied normal strain.


Langmuir | 2018

Layer-by-Layer Assembled Microgels Can Combine Conflicting Properties: Switchable Stiffness and Wettability without Affecting Permeability

Eliana Maza; Catalina von Bilderling; M. Lorena Cortez; Gisela Díaz; Micaela Bianchi; Lía I. Pietrasanta; Juan M. Giussi; Omar Azzaroni

Responsive interfacial architectures of practical interest commonly require the combination of conflicting properties in terms of their demand upon material structure. Switchable stiffness, wettability, and permeability, key features for tissue engineering applications, are in fact known to be exclusively interdependent. Here, we present a nanoarchitectonic approach that decouples these divergent properties by the use of thermoresponsive microgels as building blocks for the construction of three-dimensional arrays of interconnected pores. Layer-by-layer assembled poly( N-isopropylacrylamide- co-methacrylic acid) microgel films were found to exhibit an increase in hydrophobicity, stiffness, and adhesion properties upon switching the temperature from below to above the lower critical solution temperature, whereas the permeability of redox probes through the film remained unchanged. Our findings indicate that the switch in hydrophilicity and nanomechanical properties undergone by the microgels does not compromise the porosity of the film, thus allowing the free diffusion of redox probes through the polymer-free volume of the submicrometer pores. This novel approach for decoupling conflicting properties provides a strategic route for creating tailorable scaffolds with unforeseen functionalities.


Advanced Optical Materials | 2013

A Nonlinear Switching Mechanism in Quantum Dot and Metallic Nanoparticle Hybrid Systems

Joel D. Cox; Mahi R. Singh; Catalina von Bilderling; Andrea V. Bragas


Cell Reports | 2016

Alternative Splicing of G9a Regulates Neuronal Differentiation.

Ana Fiszbein; Luciana E. Giono; Ana Quaglino; Bruno G. Berardino; Lorena Sigaut; Catalina von Bilderling; Ignacio E. Schor; Juliana H. Enriqué Steinberg; Mario Rossi; Lía I. Pietrasanta; Julio J. Caramelo; Anabella Srebrow; Alberto R. Kornblihtt


Nano Letters | 2016

Light-Induced Polarization-Directed Growth of Optically Printed Gold Nanoparticles

Ianina L. Violi; Julián Gargiulo; Catalina von Bilderling; Emiliano Cortés; Fernando D. Stefani

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Lía I. Pietrasanta

Facultad de Ciencias Exactas y Naturales

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Omar Azzaroni

National University of La Plata

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Andrea V. Bragas

Facultad de Ciencias Exactas y Naturales

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Juan M. Giussi

National University of La Plata

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Eliana Maza

National University of La Plata

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Fernando D. Stefani

National Scientific and Technical Research Council

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Lorena Sigaut

National Scientific and Technical Research Council

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M. Lorena Cortez

National Scientific and Technical Research Council

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Waldemar A. Marmisollé

National Scientific and Technical Research Council

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Alfredo Cáceres

National Scientific and Technical Research Council

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