Caterina G.C.M. Netto

Association of Pseudomonas putida formaldehyde dehydrogenase with superparamagnetic nanoparticles: an effective way of improving the enzyme stability, performance and recycling

The association of formaldehyde dehydrogenase from Pseudomonas putida (FalDH) with two different magnetic nanoparticles led to distinct enzyme responses depending upon the chemical modification process applied. The magnetite nanoparticles coated with aminopropyltriethoxysilane (MagNP-APTS) exhibited a lower activity and stability, while magnetite coated with a silica shell and APTS (MagNP@SiO2APTS) afforded excellent results, improving the stability and performance of FalDH in relation to the free enzyme. Such differences were ascribed to unfavorable conformational changes in the MagNP-APTS–FalDH system, as indicated by the kinetics and Raman spectroscopy data, not discarding the possible interference from the exposed Fe(II)/(III) ions. The MagNP@SiO2APTS–FalDH catalyst could be successfully recycled by using an external magnet, keeping its highest performance close to 100%, during the first four cycles, and decaying slightly up to 70% after the 10th cycle.

Correction: Bovine glutamate dehydrogenase immobilization on magnetic nanoparticles: conformational changes and catalysis

Correction for ‘Bovine glutamate dehydrogenase immobilization on magnetic nanoparticles: conformational changes and catalysis’ by Caterina G. C. Marques Netto et al., RSC Adv., 2016, 6, 12977–12992.

Enzymatic reactions involving the heteroatoms from organic substrates

Several enzymatic reactions of heteroatom-containing compounds have been explored as unnatural substrates. Considerable advances related to the search for efficient enzymatic systems able to support a broader substrate scope with high catalytic performance are described in the literature. These reports include mainly native and mutated enzymes and whole cells biocatalysis. Herein, we describe the historical background along with the progress of biocatalyzed reactions involving the heteroatom(S, Se, B, P and Si) from hetero-organic substrates.